Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 66044532

GI number: 66044532

Start: 1449781

End: 1450533

Strand: Direct

Name: trmD

Synonym: Psyr_1284

Alternate gene names: 66044532

Gene position: 1449781-1450533 (Clockwise)

Preceding gene: 66044531

Following gene: 66044533

Centisome position: 23.79

GC content: 56.71

Gene sequence:

>753_bases
ATGGCCAGCCTGCGCATAGAAGTCATCAGTCTGTTTCCCGAGATGTTTTCCGCCATCAGCGAATACGGCATTACCAGCCG
TGCGGTGAAACAAGGGCTGTTGCAGCTCACTTGTTGGAATCCGCGGGATTACACCACTGATCGCCATCACACTGTGGATG
ATCGCCCATTTGGCGGTGGTCCGGGCATGGTGATGAAGATCAAGCCCCTTGAAGATGCTCTGGTTCAGGCCAGGCAAGCG
GCAGGGGATGCGGCGAAGGTGATTTACCTGTCGCCACAAGGCCGCCAGCTGAATCAGTCTGCGGTACGCGAACTGGCGCA
GGAGGAAGCGATTATCCTCATCGCCGGTCGTTATGAAGGCATTGACGAGCGTTTTATTGATGCTCATGTCGATGAAGAGT
GGTCGATTGGTGACTATGTTCTTTCCGGTGGCGAGCTGCCGGCGATGGTCCTGATTGACGCGGTTACGCGGCTGCTGCCT
GGAGCTTTAGGGCATGTGGACTCCGCGGAGGAAGATTCCTTCACGGACGGTCTGCTGGATTGCCCGCATTACACCCGACC
TGAGGTGTATGCGGATCAGCGTGTTCCCGACGTGTTGCTAAGTGGCAATCACGCGCACATCCGGCGTTGGCGTTTACAGC
AGTCCCTTGGGCGGACCTATGAACGACGCGCTGATCTTCTGGAAAGCCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTG
GCGGAATACATCCGCGAGCGGGACGATAGTTAA

Upstream 100 bases:

>100_bases
ATCGCGAACGCCTGTTGCCCTATACGGAGCAATGCGTGTTGGCAATCGATATGGCTGCTGGCGAGATGAAGGTGGATTGG
GACGCGGACTTCTGAATGCC

Downstream 100 bases:

>100_bases
CGTATCGATGGTCGATCCAGACGGATTACCTTAGGAGCACCAGCATGACTAACAAAATCATTCTTGCCCTCGAAGCAGAG
CAGATGACCAAAGAGATCCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MASLRIEVISLFPEMFSAISEYGITSRAVKQGLLQLTCWNPRDYTTDRHHTVDDRPFGGGPGMVMKIKPLEDALVQARQA
AGDAAKVIYLSPQGRQLNQSAVRELAQEEAIILIAGRYEGIDERFIDAHVDEEWSIGDYVLSGGELPAMVLIDAVTRLLP
GALGHVDSAEEDSFTDGLLDCPHYTRPEVYADQRVPDVLLSGNHAHIRRWRLQQSLGRTYERRADLLESRSLSGEEKKLL
AEYIRERDDS

Sequences:

>Translated_250_residues
MASLRIEVISLFPEMFSAISEYGITSRAVKQGLLQLTCWNPRDYTTDRHHTVDDRPFGGGPGMVMKIKPLEDALVQARQA
AGDAAKVIYLSPQGRQLNQSAVRELAQEEAIILIAGRYEGIDERFIDAHVDEEWSIGDYVLSGGELPAMVLIDAVTRLLP
GALGHVDSAEEDSFTDGLLDCPHYTRPEVYADQRVPDVLLSGNHAHIRRWRLQQSLGRTYERRADLLESRSLSGEEKKLL
AEYIRERDDS
>Mature_249_residues
ASLRIEVISLFPEMFSAISEYGITSRAVKQGLLQLTCWNPRDYTTDRHHTVDDRPFGGGPGMVMKIKPLEDALVQARQAA
GDAAKVIYLSPQGRQLNQSAVRELAQEEAIILIAGRYEGIDERFIDAHVDEEWSIGDYVLSGGELPAMVLIDAVTRLLPG
ALGHVDSAEEDSFTDGLLDCPHYTRPEVYADQRVPDVLLSGNHAHIRRWRLQQSLGRTYERRADLLESRSLSGEEKKLLA
EYIRERDDS

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=66.804979253112, Blast_Score=327, Evalue=4e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_PSE14 (Q48LV8)

Other databases:

- EMBL:   CP000058
- RefSeq:   YP_273614.1
- ProteinModelPortal:   Q48LV8
- SMR:   Q48LV8
- STRING:   Q48LV8
- GeneID:   3557662
- GenomeReviews:   CP000058_GR
- KEGG:   psp:PSPPH_1356
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q48LV8
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28033; Mature: 27902

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLRIEVISLFPEMFSAISEYGITSRAVKQGLLQLTCWNPRDYTTDRHHTVDDRPFGGG
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCC
PGMVMKIKPLEDALVQARQAAGDAAKVIYLSPQGRQLNQSAVRELAQEEAIILIAGRYEG
CCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCC
IDERFIDAHVDEEWSIGDYVLSGGELPAMVLIDAVTRLLPGALGHVDSAEEDSFTDGLLD
CHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCC
CPHYTRPEVYADQRVPDVLLSGNHAHIRRWRLQQSLGRTYERRADLLESRSLSGEEKKLL
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AEYIRERDDS
HHHHHHCCCC
>Mature Secondary Structure 
ASLRIEVISLFPEMFSAISEYGITSRAVKQGLLQLTCWNPRDYTTDRHHTVDDRPFGGG
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCC
PGMVMKIKPLEDALVQARQAAGDAAKVIYLSPQGRQLNQSAVRELAQEEAIILIAGRYEG
CCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCC
IDERFIDAHVDEEWSIGDYVLSGGELPAMVLIDAVTRLLPGALGHVDSAEEDSFTDGLLD
CHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCC
CPHYTRPEVYADQRVPDVLLSGNHAHIRRWRLQQSLGRTYERRADLLESRSLSGEEKKLL
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AEYIRERDDS
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA