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The map label for this gene is ampD [H]

Identifier: 66044072

GI number: 66044072

Start: 923198

End: 923746

Strand: Direct

Name: ampD [H]

Synonym: Psyr_0817

Alternate gene names: 66044072

Gene position: 923198-923746 (Clockwise)

Preceding gene: 66044071

Following gene: 66044073

Centisome position: 15.15

GC content: 59.02

Gene sequence:

>549_bases
ATGCAACTGGACTCCGCAAGCGGCTGGTGCGACGGCGTGCAGCATTGCCCCTCGCCCAACTTCAATGCGCGACCCGAAGG
CGAGATCTCGCTGCTGGTTATCCACAACATCAGCCTGCCGCCAGCGCAGTTCAAGACCGGCAAGGTGCAGGCTTTCTTTC
AGAACCAGCTCGACATCGACGAGCATCCCTACTTTGTGGGGATTGCCGATCTGCGCGTTTCAGCGCATTTTCTGATCGAA
CGTGATGGCGAGGTCATTCAGTTCGTCTCTTGTCTTGATCGTGCGTGGCACGCCGGTGTTTCCAGTTTTCAGGGCCGGGA
GGGGTGCAATGACTTCTCTGTGGGCATTGAGCTGGAGGGGACGGATGACCAGCCTTTCACTGATGCGCAATACGACGCGC
TGATCGACCTGACGCGACAGTTGCGTCAGGCGTTCGAGGCGATCACCCCCGAGCGTATCTGTGGTCACAGTGACGTGGCG
CCGGGACGCAAGACTGATCCTGGGCCCTGTTTTGACTGGGCCAGGTTTCGAGCGGCTTTGCTGGATTGA

Upstream 100 bases:

>100_bases
GTGTGTTTCCTACATTGCGGATCCGTACCCAAGCCCCCACATCGGCTCCTGCCACGGGCATAATCAGCGCTACTTATTTT
CCCCCGTTCAAGGAGCATTC

Downstream 100 bases:

>100_bases
TGTGAGGGACGAACAATGAGTTTTCTGGTGCTACTGCTGGCCGTTCTTATCGAAAAGTTTTCTGCGCTGCGTCAGCGCGT
TCAGCGTGACGGACCGTGGC

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MQLDSASGWCDGVQHCPSPNFNARPEGEISLLVIHNISLPPAQFKTGKVQAFFQNQLDIDEHPYFVGIADLRVSAHFLIE
RDGEVIQFVSCLDRAWHAGVSSFQGREGCNDFSVGIELEGTDDQPFTDAQYDALIDLTRQLRQAFEAITPERICGHSDVA
PGRKTDPGPCFDWARFRAALLD

Sequences:

>Translated_182_residues
MQLDSASGWCDGVQHCPSPNFNARPEGEISLLVIHNISLPPAQFKTGKVQAFFQNQLDIDEHPYFVGIADLRVSAHFLIE
RDGEVIQFVSCLDRAWHAGVSSFQGREGCNDFSVGIELEGTDDQPFTDAQYDALIDLTRQLRQAFEAITPERICGHSDVA
PGRKTDPGPCFDWARFRAALLD
>Mature_182_residues
MQLDSASGWCDGVQHCPSPNFNARPEGEISLLVIHNISLPPAQFKTGKVQAFFQNQLDIDEHPYFVGIADLRVSAHFLIE
RDGEVIQFVSCLDRAWHAGVSSFQGREGCNDFSVGIELEGTDDQPFTDAQYDALIDLTRQLRQAFEAITPERICGHSDVA
PGRKTDPGPCFDWARFRAALLD

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=174, Percent_Identity=55.1724137931034, Blast_Score=196, Evalue=9e-52,
Organism=Escherichia coli, GI1787092, Length=125, Percent_Identity=37.6, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20237; Mature: 20237

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDSASGWCDGVQHCPSPNFNARPEGEISLLVIHNISLPPAQFKTGKVQAFFQNQLDID
CCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCC
EHPYFVGIADLRVSAHFLIERDGEVIQFVSCLDRAWHAGVSSFQGREGCNDFSVGIELEG
CCCEEEEEEHHEEEEEEEEECCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEEEEEC
TDDQPFTDAQYDALIDLTRQLRQAFEAITPERICGHSDVAPGRKTDPGPCFDWARFRAAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
LD
CC
>Mature Secondary Structure
MQLDSASGWCDGVQHCPSPNFNARPEGEISLLVIHNISLPPAQFKTGKVQAFFQNQLDID
CCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCC
EHPYFVGIADLRVSAHFLIERDGEVIQFVSCLDRAWHAGVSSFQGREGCNDFSVGIELEG
CCCEEEEEEHHEEEEEEEEECCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEEEEEC
TDDQPFTDAQYDALIDLTRQLRQAFEAITPERICGHSDVAPGRKTDPGPCFDWARFRAAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
LD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]