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The map label for this gene is braE [H]

Identifier: 66043866

GI number: 66043866

Start: 687919

End: 689232

Strand: Direct

Name: braE [H]

Synonym: Psyr_0599

Alternate gene names: 66043866

Gene position: 687919-689232 (Clockwise)

Preceding gene: 66043865

Following gene: 66043867

Centisome position: 11.29

GC content: 60.65

Gene sequence:

>1314_bases
ATGTCCGCTCCTGTTGCTGTTCCTGTTTCCAAACCTGTCGAAATCAAGAAGAGTCTGATCGATGCCGTAGTCGCCGGGTT
GCTGGCGCTGATCGTATTCGGCCCCATTGTCGGGATCGTGCTCGATGGCTACAGCTTCAACCTGCAACCGACGCGCGTGG
CCTGGCTGGTGGGCGTGGTCATGATCGGTCGCTTTCTGATCAGTCTGTTTCTGCAAACACCCAGAGGCCTGCGTGTTTCC
CAGAGCTTCGAAAGCTCCGACTCCGGCGTGCATGTGCTCAAACCGGATCATCGATCAAGCCTGTACTGGATCATTCCGCT
GCTGATCGTGATTGCCATCGTGTTCCCGATCTTCGCCAACAAGTACATTCTGACCGTGGTCATCCTCGGTCTGATCTACG
TGCTGCTGGGCCTGGGGCTGAACATCGTGGTCGGTCTGGCGGGGCTGCTCGACCTTGGCTACGTGGCGTTTTACGCCATC
GGTGCTTATGGTCTGGCGCTGGGCTATCAGTATCTGGGCCTGGGCTTCTGGTCGGCCTTGCCGCTGGCGGCGATCGCGGC
GGCGCTGGCCGGGTGCATTCTCGGCTTCCCGGTGTTGCGCATGCATGGCGACTATCTGGCCATCGTGACGCTAGGGTTCG
GCGAGATCATTCGCCTTGTGTTGAATAACTGGCTGTCGTTCACCGGCGGCCCGAACGGTGTGCCGGTGCCGGCGCCCACC
TTCCTCGGGCTGGAGTTTGGCCGACGCGCCAAGGACGGCGGGGTGCCGATCCATGAATATTTCGGCTTCGATTACAACCC
GGACCTGAAATTCATCTTCATCTACACCGTGCTGTTCCTGGTCGTGCTGGCGGTGCTGTTCATCAAGCATCGCCTGACCC
GCATGCCGATCGGGCGTGCCTGGGAAGCCTTGCGCGAGGACGAGATCGCCTGTCGTTCGATGGGCCTCAATCATGTGCTG
GTCAAGCTGTCGGCGTTCACCATCGGTGCGTCGACCGCAGGCCTTGCCGGAGTGTTTTTCGCCAGCTATCAGGGCTTCGT
CAACCCGACCTCATTCACCTTTTTCGAGTCGGCGCTGATTCTGGCGATCGTGGTGCTGGGCGGCATGGGCTCGACCGTCG
GTGTGGTGATCGCCGCGTTCGTGCTGACCGTTGCGCCTGAACTGCTGCGCAGCTTCTCCGAATACCGCGTGCTGCTGTTC
GGGATTCTGATGGTGCTGATGATGATCTGGCGACCACGCGGCCTGATTCGCATCAGCCGCACCGGGGTAAAACCGCGCAA
GGGCGCGCTGGTGACAGAGAGGGCTGCGCGATGA

Upstream 100 bases:

>100_bases
CCGATTACAAAGACGTGTTCAGTTTCGGCCTGCTGGTGCTGATCCTGATTTTCCGTCCCCAAGGCCTGCTGGGTCGCCCA
CTCGTGGCGAAGGTATAAGT

Downstream 100 bases:

>100_bases
GCACTGAAGTGATTCTCTCCGTCGAGCACCTGATGATGCACTTTGGCGGCATCAAGGCCCTCAGCGACGTCAGCCTCAAG
GTCCGGCGCAATTCGATTTT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSAPVAVPVSKPVEIKKSLIDAVVAGLLALIVFGPIVGIVLDGYSFNLQPTRVAWLVGVVMIGRFLISLFLQTPRGLRVS
QSFESSDSGVHVLKPDHRSSLYWIIPLLIVIAIVFPIFANKYILTVVILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAI
GAYGLALGYQYLGLGFWSALPLAAIAAALAGCILGFPVLRMHGDYLAIVTLGFGEIIRLVLNNWLSFTGGPNGVPVPAPT
FLGLEFGRRAKDGGVPIHEYFGFDYNPDLKFIFIYTVLFLVVLAVLFIKHRLTRMPIGRAWEALREDEIACRSMGLNHVL
VKLSAFTIGASTAGLAGVFFASYQGFVNPTSFTFFESALILAIVVLGGMGSTVGVVIAAFVLTVAPELLRSFSEYRVLLF
GILMVLMMIWRPRGLIRISRTGVKPRKGALVTERAAR

Sequences:

>Translated_437_residues
MSAPVAVPVSKPVEIKKSLIDAVVAGLLALIVFGPIVGIVLDGYSFNLQPTRVAWLVGVVMIGRFLISLFLQTPRGLRVS
QSFESSDSGVHVLKPDHRSSLYWIIPLLIVIAIVFPIFANKYILTVVILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAI
GAYGLALGYQYLGLGFWSALPLAAIAAALAGCILGFPVLRMHGDYLAIVTLGFGEIIRLVLNNWLSFTGGPNGVPVPAPT
FLGLEFGRRAKDGGVPIHEYFGFDYNPDLKFIFIYTVLFLVVLAVLFIKHRLTRMPIGRAWEALREDEIACRSMGLNHVL
VKLSAFTIGASTAGLAGVFFASYQGFVNPTSFTFFESALILAIVVLGGMGSTVGVVIAAFVLTVAPELLRSFSEYRVLLF
GILMVLMMIWRPRGLIRISRTGVKPRKGALVTERAAR
>Mature_436_residues
SAPVAVPVSKPVEIKKSLIDAVVAGLLALIVFGPIVGIVLDGYSFNLQPTRVAWLVGVVMIGRFLISLFLQTPRGLRVSQ
SFESSDSGVHVLKPDHRSSLYWIIPLLIVIAIVFPIFANKYILTVVILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIG
AYGLALGYQYLGLGFWSALPLAAIAAALAGCILGFPVLRMHGDYLAIVTLGFGEIIRLVLNNWLSFTGGPNGVPVPAPTF
LGLEFGRRAKDGGVPIHEYFGFDYNPDLKFIFIYTVLFLVVLAVLFIKHRLTRMPIGRAWEALREDEIACRSMGLNHVLV
KLSAFTIGASTAGLAGVFFASYQGFVNPTSFTFFESALILAIVVLGGMGSTVGVVIAAFVLTVAPELLRSFSEYRVLLFG
ILMVLMMIWRPRGLIRISRTGVKPRKGALVTERAAR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=323, Percent_Identity=57.2755417956656, Blast_Score=343, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 47290; Mature: 47159

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
SAPVAVPVSKPVEIKKSLIDAVVAGLLALIVFGPIVGIVLDGYSFNLQPTRVAWLVGVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MIGRFLISLFLQTPRGLRVSQSFESSDSGVHVLKPDHRSSLYWIIPLLIVIAIVFPIFAN
HHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
KYILTVVILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAYGLALGYQYLGLGFWSAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLAAIAAALAGCILGFPVLRMHGDYLAIVTLGFGEIIRLVLNNWLSFTGGPNGVPVPAPT
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
FLGLEFGRRAKDGGVPIHEYFGFDYNPDLKFIFIYTVLFLVVLAVLFIKHRLTRMPIGRA
HHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
WEALREDEIACRSMGLNHVLVKLSAFTIGASTAGLAGVFFASYQGFVNPTSFTFFESALI
HHHHHHHHHHHHHCCHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LAIVVLGGMGSTVGVVIAAFVLTVAPELLRSFSEYRVLLFGILMVLMMIWRPRGLIRISR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
TGVKPRKGALVTERAAR
CCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]