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The map label for this gene is braF [H]

Identifier: 66043867

GI number: 66043867

Start: 689229

End: 690104

Strand: Direct

Name: braF [H]

Synonym: Psyr_0600

Alternate gene names: 66043867

Gene position: 689229-690104 (Clockwise)

Preceding gene: 66043866

Following gene: 66043868

Centisome position: 11.31

GC content: 59.93

Gene sequence:

>876_bases
ATGAGCACTGAAGTGATTCTCTCCGTCGAGCACCTGATGATGCACTTTGGCGGCATCAAGGCCCTCAGCGACGTCAGCCT
CAAGGTCCGGCGCAATTCGATTTTCGCCCTGATCGGCCCCAACGGTGCGGGCAAGACCACGGTCTTCAACTGCCTGACCG
GTTTCTACAAGGCCACTGGCGGACGCATCGAACTGCACACTCGTGGCAAGACCACCAATGTGATCAAGCTATTGGGCGAG
CCGTTCCAGGCGACCGACTTCGTGTCGCCGAAAAGCTTCCTCAGCCGGGTGTACTACAAGATGTTCGGCGGCACCCATCT
GGTCAACAGGGCAGGGCTGGCGCGCACGTTCCAGAACATTCGCCTGTTCAAGGAAATGTCGGTCGTCGAAAACCTGCTGG
TCGCCCAGCACATGTGGGTCAACCGCAGCCTGATCAGCGGGATCCTGAATACCAGGGGCTATCGCAAGGCCGAAGAGGAT
GCGCTGAACACCGCGTTCTACTGGCTGGAGGTGGTCGATCTGGTGGACTGCGCCAACCGTCTGGCTGGCGAGTTGTCCTA
CGGGCAGCAGCGCCGTCTGGAGATTGCGCGAGCCATGTGCACGCGGCCGCAGGTGATCTGCCTGGATGAGCCGGCTGCCG
GTCTCAACCCGCAGGAAACCGAGGCACTCAGCGGCATGATCCGCCTGCTGCGTGACGAGCACGACATGACCATTGTGCTG
ATCGAGCACGACATGGGCATGGTCATGGGGATTTCCGACGATATTGTGGTGCTCGACCATGGCAACGTGATTGCGATGGG
CGGGCCGGAACAGATCCGTAATGATCCGAAGGTGATCGCTGCGTATCTGGGCGCTGATGAAGAGGAGCTGGTATGA

Upstream 100 bases:

>100_bases
TGGTGCTGATGATGATCTGGCGACCACGCGGCCTGATTCGCATCAGCCGCACCGGGGTAAAACCGCGCAAGGGCGCGCTG
GTGACAGAGAGGGCTGCGCG

Downstream 100 bases:

>100_bases
GCAAGCCGATCCTCGAACTGAAAGAGATCGATGTGTTTTACGGGCCGATTCAGGCCCTGAAGAAAGTCTCGCTGCACATC
AATGAGGGTGAAACGGTGAG

Product: ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSTEVILSVEHLMMHFGGIKALSDVSLKVRRNSIFALIGPNGAGKTTVFNCLTGFYKATGGRIELHTRGKTTNVIKLLGE
PFQATDFVSPKSFLSRVYYKMFGGTHLVNRAGLARTFQNIRLFKEMSVVENLLVAQHMWVNRSLISGILNTRGYRKAEED
ALNTAFYWLEVVDLVDCANRLAGELSYGQQRRLEIARAMCTRPQVICLDEPAAGLNPQETEALSGMIRLLRDEHDMTIVL
IEHDMGMVMGISDDIVVLDHGNVIAMGGPEQIRNDPKVIAAYLGADEEELV

Sequences:

>Translated_291_residues
MSTEVILSVEHLMMHFGGIKALSDVSLKVRRNSIFALIGPNGAGKTTVFNCLTGFYKATGGRIELHTRGKTTNVIKLLGE
PFQATDFVSPKSFLSRVYYKMFGGTHLVNRAGLARTFQNIRLFKEMSVVENLLVAQHMWVNRSLISGILNTRGYRKAEED
ALNTAFYWLEVVDLVDCANRLAGELSYGQQRRLEIARAMCTRPQVICLDEPAAGLNPQETEALSGMIRLLRDEHDMTIVL
IEHDMGMVMGISDDIVVLDHGNVIAMGGPEQIRNDPKVIAAYLGADEEELV
>Mature_290_residues
STEVILSVEHLMMHFGGIKALSDVSLKVRRNSIFALIGPNGAGKTTVFNCLTGFYKATGGRIELHTRGKTTNVIKLLGEP
FQATDFVSPKSFLSRVYYKMFGGTHLVNRAGLARTFQNIRLFKEMSVVENLLVAQHMWVNRSLISGILNTRGYRKAEEDA
LNTAFYWLEVVDLVDCANRLAGELSYGQQRRLEIARAMCTRPQVICLDEPAAGLNPQETEALSGMIRLLRDEHDMTIVLI
EHDMGMVMGISDDIVVLDHGNVIAMGGPEQIRNDPKVIAAYLGADEEELV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI1789864, Length=282, Percent_Identity=51.063829787234, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1788761, Length=270, Percent_Identity=27.4074074074074, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789672, Length=273, Percent_Identity=24.9084249084249, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082268, Length=284, Percent_Identity=28.8732394366197, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787029, Length=265, Percent_Identity=27.5471698113208, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790190, Length=262, Percent_Identity=30.9160305343511, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48995001, Length=259, Percent_Identity=29.7297297297297, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788472, Length=283, Percent_Identity=30.0353356890459, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788225, Length=271, Percent_Identity=28.0442804428044, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789593, Length=282, Percent_Identity=27.6595744680851, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1786872, Length=276, Percent_Identity=24.6376811594203, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87081709, Length=286, Percent_Identity=23.0769230769231, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787712, Length=271, Percent_Identity=25.0922509225092, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789991, Length=260, Percent_Identity=27.6923076923077, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790544, Length=279, Percent_Identity=28.3154121863799, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081791, Length=282, Percent_Identity=24.113475177305, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1789032, Length=270, Percent_Identity=25.5555555555556, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788210, Length=284, Percent_Identity=27.4647887323944, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787370, Length=271, Percent_Identity=24.3542435424354, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787089, Length=252, Percent_Identity=25.7936507936508, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788450, Length=265, Percent_Identity=26.0377358490566, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790525, Length=260, Percent_Identity=28.8461538461538, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788644, Length=278, Percent_Identity=27.3381294964029, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790162, Length=277, Percent_Identity=26.7148014440433, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI48994997, Length=276, Percent_Identity=25.7246376811594, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786398, Length=263, Percent_Identity=26.2357414448669, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786703, Length=249, Percent_Identity=26.1044176706827, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI48994943, Length=271, Percent_Identity=25.4612546125461, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787758, Length=258, Percent_Identity=22.8682170542636, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790467, Length=284, Percent_Identity=23.5915492957746, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI145693107, Length=279, Percent_Identity=22.9390681003584, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788165, Length=107, Percent_Identity=33.6448598130841, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787500, Length=289, Percent_Identity=21.7993079584775, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789586, Length=259, Percent_Identity=28.1853281853282, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786345, Length=272, Percent_Identity=23.8970588235294, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788506, Length=102, Percent_Identity=27.4509803921569, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786981, Length=242, Percent_Identity=28.5123966942149, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787105, Length=260, Percent_Identity=23.0769230769231, Blast_Score=61, Evalue=7e-11,
Organism=Drosophila melanogaster, GI221512771, Length=265, Percent_Identity=28.3018867924528, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648314, Length=281, Percent_Identity=24.9110320284698, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 32315; Mature: 32184

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEVILSVEHLMMHFGGIKALSDVSLKVRRNSIFALIGPNGAGKTTVFNCLTGFYKATG
CCCEEEEEHHHHHHHHCCHHHHHHCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHCCC
GRIELHTRGKTTNVIKLLGEPFQATDFVSPKSFLSRVYYKMFGGTHLVNRAGLARTFQNI
CEEEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RLFKEMSVVENLLVAQHMWVNRSLISGILNTRGYRKAEEDALNTAFYWLEVVDLVDCANR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAGELSYGQQRRLEIARAMCTRPQVICLDEPAAGLNPQETEALSGMIRLLRDEHDMTIVL
HHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
IEHDMGMVMGISDDIVVLDHGNVIAMGGPEQIRNDPKVIAAYLGADEEELV
EECCCCEEEECCCCEEEEECCCEEEECCHHHHCCCHHEEEHHHCCCHHHCC
>Mature Secondary Structure 
STEVILSVEHLMMHFGGIKALSDVSLKVRRNSIFALIGPNGAGKTTVFNCLTGFYKATG
CCEEEEEHHHHHHHHCCHHHHHHCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHCCC
GRIELHTRGKTTNVIKLLGEPFQATDFVSPKSFLSRVYYKMFGGTHLVNRAGLARTFQNI
CEEEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RLFKEMSVVENLLVAQHMWVNRSLISGILNTRGYRKAEEDALNTAFYWLEVVDLVDCANR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAGELSYGQQRRLEIARAMCTRPQVICLDEPAAGLNPQETEALSGMIRLLRDEHDMTIVL
HHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
IEHDMGMVMGISDDIVVLDHGNVIAMGGPEQIRNDPKVIAAYLGADEEELV
EECCCCEEEECCCCEEEEECCCEEEECCHHHHCCCHHEEEHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]