Click here to switch to the map view.

The map label for this gene is thiC [H]

Identifier: 66043811

GI number: 66043811

Start: 615173

End: 617062

Strand: Direct

Name: thiC [H]

Synonym: Psyr_0544

Alternate gene names: 66043811

Gene position: 615173-617062 (Clockwise)

Preceding gene: 66043809

Following gene: 66043812

Centisome position: 10.1

GC content: 61.27

Gene sequence:

>1890_bases
ATGAGTATAAAAGCAAAAAACGCAGCCCATTTGCGCGAGTCGGCGCAGGTCGATTCCGGATCGGTACAGCCGTTTACCCG
TTCGCAGAAAATCTACGTTCAGGGCTCGCGCCCGGACATCCGCGTGCCGATGCGCGAAATCACCCTGGATGTCACCCCGA
CTGACTTTGGCGGCGAGATCAACGCCCCGGTCACGGTCTATGACACCTCGGGGCCCTACACCGACCCCAATGTGATCATC
GACGTGCGCAAAGGCCTGGCTGACGTGCGCTCGCCATGGATCGACTCGCGCAATGACACCGAGCGTCTGCCGGGCCTGAG
TTCCAACTTCGGCCAGCAACGCCTGAGCGATGCCGAGCTCACCGCGCTGCGCTTTGCCCATGTGCGCAACCCGCGCCGGG
CCAAGGCCGGTGCCAACGTCAGCCAGATGCACTATGCGCGTCAGGGCATCATTACCGCCGAGATGGAATACGTCGCCATC
CGCGAGAACATGAAGCTTCAGGAAGCCCGCGCCGCAGGTCTGCTGACCCAGCAACATGCCGGGCACAGCTTTGGCGCGAG
TATTCCGAAGGAAATCACTGCCGAGTTCGTGCGTGAAGAGATTGCCCGGGGCCGCGCGATCATCCCGGCCAACATCAACC
ACGTCGAGCTTGAGCCGATGATCATCGGCCGCAACTTCCTGGTGAAGATCAACGGCAACATCGGCAACAGCGCACTGGGC
TCTTCCATTGAAGAAGAGGTCGCCAAGCTGACCTGGGGTATCCGCTGGGGCTCGGACACGGTCATGGACCTGTCCACCGG
CAAGCACATTCACGAAACCCGTGAGTGGATCATCCGCAATTCGCCGGTGCCGATCGGTACGGTGCCGATCTATCAGGCGC
TGGAAAAAGTGGGGGGTGCAGCCGAGGACCTGACCTGGGAGCTGTTCCGCGACACGCTGATCGAGCAGGCCGAGCAGGGC
GTCGATTACTTCACCATCCACGCCGGAGTGCTGCTGCGTTACGTGCCGCTGACCGCCAAGCGTGTCACCGGTATCGTCAG
CCGTGGCGGTTCGATCATGGCCAAATGGTGCCTGGCGCACCACAAGGAAAACTTCCTTTACACCCATTTCGAAGACATCT
GCGAAATCATGAAGGCCTACGACGTCAGCTTCTCGCTGGGCGACGGCCTGCGTCCGGGCTCGATTGCCGACGCCAACGAT
GCGGCGCAGTTCGGCGAACTGGAAACGCTTGGCGAACTGACCAAGATTGCCTGGAAACATGACGTGCAGACCATGATCGA
AGGCCCCGGCCATGTGCCGATGCAGTTGATCAAGGAAAACATGGACAAGCAGCTGGAATGCTGCGACGAAGCGCCGTTCT
ACACCCTGGGCCCGCTGACCACCGACATCGCGCCGGGTTACGACCACATCACCTCGGGCATCGGCGCGGCCATGATCGGC
TGGTTCGGCTGCGCCATGCTGTGTTACGTCACGCCCAAGGAGCATCTGGGGCTGCCGAACAAGGATGACGTCAAAACCGG
GATCATCACCTACAAGATCGCGGCCCATGCGGCGGACCTTGCCAAAGGCCACCCGGGTGCGCAGATCCGTGACAACGCGT
TGAGCAAGGCGCGTTTCGAGTTCCGCTGGGAAGACCAGTTCAACCTCGGCCTGGACCCGGACACCGCGCGTTCGTACCAC
GACGAAACCCTGCCCAAGGATTCGGCCAAGGTCGCGCACTTCTGCTCCATGTGCGGGCCGAAATTCTGCTCGATGAAGAT
CACTCAGGAAGTCCGCGAGTACGCCGCCAACCAGCGCATCGAGGCGGTAGACGTCGATGTCGCCAAGGGCCTGGCCGAAC
AGGCCGAGCGCTTCAAGCAGGAGGGTAGTCAGCTGTACAAGAAGGTGTAA

Upstream 100 bases:

>100_bases
TTCGCCTCCTTTCGCTACTCAGCACCAGCACTGGTGCACCCGTCCGTCATGTCAGGTCACTCCGACAATAAATCCGCAAC
TGGATGTCTGGAGAGCCGTG

Downstream 100 bases:

>100_bases
AGGGCGATAGCCCGTCGTTATACACAGTTCCGCTTTGATTCTGCCCGAAGGGCGTAGGAGCGACCGGGGCGGCGATCCGC
ATTGTTCGCGAAAGCTTTAT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSIKAKNAAHLRESAQVDSGSVQPFTRSQKIYVQGSRPDIRVPMREITLDVTPTDFGGEINAPVTVYDTSGPYTDPNVII
DVRKGLADVRSPWIDSRNDTERLPGLSSNFGQQRLSDAELTALRFAHVRNPRRAKAGANVSQMHYARQGIITAEMEYVAI
RENMKLQEARAAGLLTQQHAGHSFGASIPKEITAEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALG
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGGAAEDLTWELFRDTLIEQAEQG
VDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIADAND
AAQFGELETLGELTKIAWKHDVQTMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARSYH
DETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRIEAVDVDVAKGLAEQAERFKQEGSQLYKKV

Sequences:

>Translated_629_residues
MSIKAKNAAHLRESAQVDSGSVQPFTRSQKIYVQGSRPDIRVPMREITLDVTPTDFGGEINAPVTVYDTSGPYTDPNVII
DVRKGLADVRSPWIDSRNDTERLPGLSSNFGQQRLSDAELTALRFAHVRNPRRAKAGANVSQMHYARQGIITAEMEYVAI
RENMKLQEARAAGLLTQQHAGHSFGASIPKEITAEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALG
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGGAAEDLTWELFRDTLIEQAEQG
VDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIADAND
AAQFGELETLGELTKIAWKHDVQTMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARSYH
DETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRIEAVDVDVAKGLAEQAERFKQEGSQLYKKV
>Mature_628_residues
SIKAKNAAHLRESAQVDSGSVQPFTRSQKIYVQGSRPDIRVPMREITLDVTPTDFGGEINAPVTVYDTSGPYTDPNVIID
VRKGLADVRSPWIDSRNDTERLPGLSSNFGQQRLSDAELTALRFAHVRNPRRAKAGANVSQMHYARQGIITAEMEYVAIR
ENMKLQEARAAGLLTQQHAGHSFGASIPKEITAEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALGS
SIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGGAAEDLTWELFRDTLIEQAEQGV
DYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIADANDA
AQFGELETLGELTKIAWKHDVQTMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGW
FGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARSYHD
ETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRIEAVDVDVAKGLAEQAERFKQEGSQLYKKV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=619, Percent_Identity=72.2132471728595, Blast_Score=940, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 69632; Mature: 69501

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKAKNAAHLRESAQVDSGSVQPFTRSQKIYVQGSRPDIRVPMREITLDVTPTDFGGEI
CCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHEEEEECCCCCCCCC
NAPVTVYDTSGPYTDPNVIIDVRKGLADVRSPWIDSRNDTERLPGLSSNFGQQRLSDAEL
CCCEEEEECCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHCCHHHH
TALRFAHVRNPRRAKAGANVSQMHYARQGIITAEMEYVAIRENMKLQEARAAGLLTQQHA
HHHHHHHHCCCHHHHCCCCHHHHHHHHHCCEEEHHHEEEHHCCCHHHHHHHHHHHHHHHC
GHSFGASIPKEITAEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALG
CCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEECEEEECCEEEEEECCCCCCHHHC
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGGA
CHHHHHHHHHHHCEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
AEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAH
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHH
HKENFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTKIAWKH
HCCCCEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
DVQTMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFE
HHHHHHHHEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCEEE
FRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRI
EEECCCCCCCCCCCHHHHHCHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
EAVDVDVAKGLAEQAERFKQEGSQLYKKV
EEEHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIKAKNAAHLRESAQVDSGSVQPFTRSQKIYVQGSRPDIRVPMREITLDVTPTDFGGEI
CCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHEEEEECCCCCCCCC
NAPVTVYDTSGPYTDPNVIIDVRKGLADVRSPWIDSRNDTERLPGLSSNFGQQRLSDAEL
CCCEEEEECCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHCCHHHH
TALRFAHVRNPRRAKAGANVSQMHYARQGIITAEMEYVAIRENMKLQEARAAGLLTQQHA
HHHHHHHHCCCHHHHCCCCHHHHHHHHHCCEEEHHHEEEHHCCCHHHHHHHHHHHHHHHC
GHSFGASIPKEITAEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALG
CCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEECEEEECCEEEEEECCCCCCHHHC
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGGA
CHHHHHHHHHHHCEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
AEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAH
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHH
HKENFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTKIAWKH
HCCCCEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
DVQTMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFE
HHHHHHHHEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCEEE
FRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRI
EEECCCCCCCCCCCHHHHHCHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
EAVDVDVAKGLAEQAERFKQEGSQLYKKV
EEEHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA