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The map label for this gene is pilT [H]

Identifier: 66043745

GI number: 66043745

Start: 516463

End: 517497

Strand: Direct

Name: pilT [H]

Synonym: Psyr_0478

Alternate gene names: 66043745

Gene position: 516463-517497 (Clockwise)

Preceding gene: 66043736

Following gene: 66043747

Centisome position: 8.48

GC content: 60.48

Gene sequence:

>1035_bases
ATGGATATTACCGAGCTGTTGGCCTTCAGTGCCAAACAGGGCGCGTCGGACTTGCACCTCTCTGCAGGTCTGCCGCCGAT
GATCCGCGTCGACGGCGATGTGCGGCGTATCAACCTGCCCCCGCTGGACGCGAAGGAGGTCAAGGCGCTGATCTACGACA
TCATGAACGACAAGCAACGGCAGGATTTCGAAGAGCGGCTGGAAACCGACTTTTCCTTTGAAGTGCCGGGCGTGGCGCGG
TTTCGGGTCAACGCCTTCAACCAGAACCGCGGTGCTGGCGCGGTATTCCGGACCATTCCCTCGAAAATCCTGAGCATGGA
AGACTTGGGCATGGGCAATGTGTTTCGCAAGATTACCGACGTGGCGCGCGGCCTGATTCTGGTTACCGGGCCGACCGGCT
CAGGCAAGTCGACCACCCTGGCGGCAATGATCGACTACCTCAATAACAATAAGCATCACCATATTCTGACCATCGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAGGTTCACCGCGACACGCTGGGCTTCTCGGA
GGCGCTGCGCTCGGCGTTGCGTGAAGACCCGGACGTGATTCTGGTCGGTGAAATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCTGCTGAAACCGGCCACCTGGTATTCGGCACGCTGCACACCACGTCAGCCGCCAAGACCATCGACCGGATC
GTCGACGTGTTTCCGGCTCAGGAAAAATCGATGATTCGCTCGATGCTCTCCGAATCGCTGCACGCCGTGGTCTCGCAGGC
GTTGCTCAAGAAGGTCGGCGGCGGGCGCGTGGCGGCGCACGAAATCATGATGGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAAGTGGCGCAGATGTATTCGTCGATCCAGACGGGTGGCTCGATGGGCATGCAGACGCTGGACATGTGCCTG
GCCGATCTGGTCAAGAAGGGGCTGATCACCCGCGAAAGCGCACGCGAACGCGCCAAGGTGCCGGATAACTTCTGA

Upstream 100 bases:

>100_bases
AATTCGCTGTTCGAGCGTTGAAATGTTCGCTGCTATCGTGGACATTGATTTGCGCCAGTCAGGCTAAGGTCTGCGGCATT
CTATGTGATTGAGGAGCTGT

Downstream 100 bases:

>100_bases
TCACAGGGATGCTGCAGGAACGAAAAAGGGGTCTTGAACGCGTTCAAGACCCCTTTTTCAGATGCAGCCTGCAGAGCGCA
GATTCAGGCCAGGATCAGCG

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQRQDFEERLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGNVFRKITDVARGLILVTGPTGSGKSTTLAAMIDYLNNNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSSIQTGGSMGMQTLDMCL
ADLVKKGLITRESARERAKVPDNF

Sequences:

>Translated_344_residues
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQRQDFEERLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGNVFRKITDVARGLILVTGPTGSGKSTTLAAMIDYLNNNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSSIQTGGSMGMQTLDMCL
ADLVKKGLITRESARERAKVPDNF
>Mature_344_residues
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQRQDFEERLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGNVFRKITDVARGLILVTGPTGSGKSTTLAAMIDYLNNNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSSIQTGGSMGMQTLDMCL
ADLVKKGLITRESARERAKVPDNF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=47.0948012232416, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1789723, Length=349, Percent_Identity=32.9512893982808, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786296, Length=383, Percent_Identity=32.1148825065274, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 37977; Mature: 37977

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHCCHHH
QDFEERLETDFSFEVPGVARFRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGNVFRKITD
HHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VARGLILVTGPTGSGKSTTLAAMIDYLNNNKHHHILTIEDPIEFVHESKKCLVNQREVHR
HHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
DTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
HHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEEHHHHHHHHHHHH
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQ
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MYSSIQTGGSMGMQTLDMCLADLVKKGLITRESARERAKVPDNF
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHCCHHH
QDFEERLETDFSFEVPGVARFRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGNVFRKITD
HHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VARGLILVTGPTGSGKSTTLAAMIDYLNNNKHHHILTIEDPIEFVHESKKCLVNQREVHR
HHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
DTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
HHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEEHHHHHHHHHHHH
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQ
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MYSSIQTGGSMGMQTLDMCLADLVKKGLITRESARERAKVPDNF
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]