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The map label for this gene is pafA

Identifier: 62390376

GI number: 62390376

Start: 1574955

End: 1576403

Strand: Reverse

Name: pafA

Synonym: cg1688

Alternate gene names: 62390376

Gene position: 1576403-1574955 (Counterclockwise)

Preceding gene: 62390377

Following gene: 62390375

Centisome position: 48.02

GC content: 51.97

Gene sequence:

>1449_bases
GTGAGTACCGTGGAATCCGCATTGACCCGCAGGATCATGGGCATTGAAACGGAGTATGGCCTCACCTTTGTTGATGGTGA
TTCCAAAAAGCTTCGCCCAGATGAGATAGCTCGAAGGATGTTTCGTCCCATCGTGGAGAAATATTCCAGCTCTAATATCT
TCATACCCAATGGTTCCCGGTTGTATCTTGATGTGGGTTCCCACCCGGAGTACGCCACCGCCGAGTGTGATAATTTGACC
CAGCTGATCAATTTTGAAAAAGCTGGCGATGTTATTGCAGATCGCATGGCTGTAGATGCCGAAGAGTCGCTGGCGAAAGA
AGACATTGCTGGGCAGGTGTACCTGTTTAAAAACAATGTCGATTCCGTGGGCAATTCTTATGGCTGCCACGAAAACTACC
TTGTGGGTCGCTCCATGCCGTTGAAGGCGTTGGGTAAAAGGCTGATGCCGTTTCTGATTACCCGCCAGCTCATCTGCGGC
GCCGGCAGGATCCATCACCCCAATCCTTTGGATAAAGGCGAATCCTTCCCCTTGGGCTACTGCATATCCCAGCGCTCTGA
CCACGTGTGGGAGGGCGTATCAAGTGCCACCACTAGATCACGCCCCATTATCAACACCCGTGATGAGCCACATGCGGATT
CCCATTCTTACCGCAGGCTGCACGTGATTGTGGGTGATGCCAACATGGCAGAGCCCAGCATCGCGTTGAAGGTCGGCTCC
ACGTTGCTGGTTCTGGAAATGATTGAGGCAGATTTCGGTTTGCCCAGCTTAGAGCTTGCCAATGATATTGCCTCAATTAG
GGAAATCTCCCGCGATGCAACAGGATCCACACTGTTGTCCCTGAAAGATGGCACCACCATGACTGCCTTGCAGATCCAGC
AGGTGGTCTTTGAGCATGCCTCGAAGTGGTTGGAGCAGCGCCCCGAACCAGAATTTTCTGGCACCTCCAACACAGAGATG
GCCCGCGTGCTGGATCTGTGGGGTCGCATGTTGAAAGCGATTGAGTCCGGTGATTTCAGCGAAGTGGATACAGAAATTGA
CTGGGTGATCAAAAAGAAGCTCATTGATCGTTTCATTCAGCGCGGCAACCTTGGGTTGGATGATCCAAAACTTGCCCAAG
TGGACTTGACTTATCACGATATTAGGCCAGGTAGAGGCCTATTTAGCGTGCTGCAAAGCCGCGGCATGATCAAACGGTGG
ACTACTGATGAGGCGATTTTAGCTGCGGTGGATACCGCTCCTGATACAACACGTGCTCATTTGCGCGGGCGAATCCTTAA
AGCGGCGGATACTCTGGGAGTACCTGTGACTGTCGATTGGATGCGTCACAAGGTCAACCGACCGGAGCCACAATCGGTGG
AATTGGGGGATCCTTTTTCCGCAGTGAATTCCGAGGTTGATCAGTTGATTGAGTATATGACCGTTCATGCGGAGAGCTAT
CGCAGTTAG

Upstream 100 bases:

>100_bases
AAGGTGTGGACAGCTTGCTGGATGAAATCGACGGACTGTTGGAAAACAACGCCGAGGAATTCGTTCGTTCCTATGTACAA
AAGGGTGGCGAATAGTCACT

Downstream 100 bases:

>100_bases
TCCGCTCGCACTTCGGAGGCCTTGATGTCGGATCGGAAGGAAGATCTCGAGCGTCAGATTAATCTGACTTTCGCGTTTTT
AAGCGCCAATTCCTATTCCC

Product: proteasome component

Products: NA

Alternate protein names: Proteasome accessory factor A; Pup-conjugating enzyme

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSTVESALTRRIMGIETEYGLTFVDGDSKKLRPDEIARRMFRPIVEKYSSSNIFIPNGSRLYLDVGSHPEYATAECDNLT
QLINFEKAGDVIADRMAVDAEESLAKEDIAGQVYLFKNNVDSVGNSYGCHENYLVGRSMPLKALGKRLMPFLITRQLICG
AGRIHHPNPLDKGESFPLGYCISQRSDHVWEGVSSATTRSRPIINTRDEPHADSHSYRRLHVIVGDANMAEPSIALKVGS
TLLVLEMIEADFGLPSLELANDIASIREISRDATGSTLLSLKDGTTMTALQIQQVVFEHASKWLEQRPEPEFSGTSNTEM
ARVLDLWGRMLKAIESGDFSEVDTEIDWVIKKKLIDRFIQRGNLGLDDPKLAQVDLTYHDIRPGRGLFSVLQSRGMIKRW
TTDEAILAAVDTAPDTTRAHLRGRILKAADTLGVPVTVDWMRHKVNRPEPQSVELGDPFSAVNSEVDQLIEYMTVHAESY
RS

Sequences:

>Translated_482_residues
MSTVESALTRRIMGIETEYGLTFVDGDSKKLRPDEIARRMFRPIVEKYSSSNIFIPNGSRLYLDVGSHPEYATAECDNLT
QLINFEKAGDVIADRMAVDAEESLAKEDIAGQVYLFKNNVDSVGNSYGCHENYLVGRSMPLKALGKRLMPFLITRQLICG
AGRIHHPNPLDKGESFPLGYCISQRSDHVWEGVSSATTRSRPIINTRDEPHADSHSYRRLHVIVGDANMAEPSIALKVGS
TLLVLEMIEADFGLPSLELANDIASIREISRDATGSTLLSLKDGTTMTALQIQQVVFEHASKWLEQRPEPEFSGTSNTEM
ARVLDLWGRMLKAIESGDFSEVDTEIDWVIKKKLIDRFIQRGNLGLDDPKLAQVDLTYHDIRPGRGLFSVLQSRGMIKRW
TTDEAILAAVDTAPDTTRAHLRGRILKAADTLGVPVTVDWMRHKVNRPEPQSVELGDPFSAVNSEVDQLIEYMTVHAESY
RS
>Mature_481_residues
STVESALTRRIMGIETEYGLTFVDGDSKKLRPDEIARRMFRPIVEKYSSSNIFIPNGSRLYLDVGSHPEYATAECDNLTQ
LINFEKAGDVIADRMAVDAEESLAKEDIAGQVYLFKNNVDSVGNSYGCHENYLVGRSMPLKALGKRLMPFLITRQLICGA
GRIHHPNPLDKGESFPLGYCISQRSDHVWEGVSSATTRSRPIINTRDEPHADSHSYRRLHVIVGDANMAEPSIALKVGST
LLVLEMIEADFGLPSLELANDIASIREISRDATGSTLLSLKDGTTMTALQIQQVVFEHASKWLEQRPEPEFSGTSNTEMA
RVLDLWGRMLKAIESGDFSEVDTEIDWVIKKKLIDRFIQRGNLGLDDPKLAQVDLTYHDIRPGRGLFSVLQSRGMIKRWT
TDEAILAAVDTAPDTTRAHLRGRILKAADTLGVPVTVDWMRHKVNRPEPQSVELGDPFSAVNSEVDQLIEYMTVHAESYR
S

Specific function: Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup- conjugating enzyme subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PAFA_CORGB (A4QE80)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138448.1
- STRING:   A4QE80
- GeneID:   4993253
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1554
- eggNOG:   NOG04175
- HOGENOM:   HBG292322
- OMA:   EYATPEC
- ProtClustDB:   CLSK872004
- HAMAP:   MF_02111
- InterPro:   IPR022279
- InterPro:   IPR004347
- PIRSF:   PIRSF018077
- TIGRFAMs:   TIGR03686

Pfam domain/function: PF03136 DUF245

EC number: NA

Molecular weight: Translated: 53802; Mature: 53670

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVESALTRRIMGIETEYGLTFVDGDSKKLRPDEIARRMFRPIVEKYSSSNIFIPNGSR
CCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCE
LYLDVGSHPEYATAECDNLTQLINFEKAGDVIADRMAVDAEESLAKEDIAGQVYLFKNNV
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCH
DSVGNSYGCHENYLVGRSMPLKALGKRLMPFLITRQLICGAGRIHHPNPLDKGESFPLGY
HHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
CISQRSDHVWEGVSSATTRSRPIINTRDEPHADSHSYRRLHVIVGDANMAEPSIALKVGS
HHCCCCCHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEECC
TLLVLEMIEADFGLPSLELANDIASIREISRDATGSTLLSLKDGTTMTALQIQQVVFEHA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
SKWLEQRPEPEFSGTSNTEMARVLDLWGRMLKAIESGDFSEVDTEIDWVIKKKLIDRFIQ
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RGNLGLDDPKLAQVDLTYHDIRPGRGLFSVLQSRGMIKRWTTDEAILAAVDTAPDTTRAH
CCCCCCCCCCEEEEEEEHCCCCCCHHHHHHHHHCCCEEECCCCHHHEEEECCCCCHHHHH
LRGRILKAADTLGVPVTVDWMRHKVNRPEPQSVELGDPFSAVNSEVDQLIEYMTVHAESY
HHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
RS
CC
>Mature Secondary Structure 
STVESALTRRIMGIETEYGLTFVDGDSKKLRPDEIARRMFRPIVEKYSSSNIFIPNGSR
CHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCE
LYLDVGSHPEYATAECDNLTQLINFEKAGDVIADRMAVDAEESLAKEDIAGQVYLFKNNV
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCH
DSVGNSYGCHENYLVGRSMPLKALGKRLMPFLITRQLICGAGRIHHPNPLDKGESFPLGY
HHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
CISQRSDHVWEGVSSATTRSRPIINTRDEPHADSHSYRRLHVIVGDANMAEPSIALKVGS
HHCCCCCHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEECC
TLLVLEMIEADFGLPSLELANDIASIREISRDATGSTLLSLKDGTTMTALQIQQVVFEHA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
SKWLEQRPEPEFSGTSNTEMARVLDLWGRMLKAIESGDFSEVDTEIDWVIKKKLIDRFIQ
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RGNLGLDDPKLAQVDLTYHDIRPGRGLFSVLQSRGMIKRWTTDEAILAAVDTAPDTTRAH
CCCCCCCCCCEEEEEEEHCCCCCCHHHHHHHHHCCCEEECCCCHHHEEEECCCCCHHHHH
LRGRILKAADTLGVPVTVDWMRHKVNRPEPQSVELGDPFSAVNSEVDQLIEYMTVHAESY
HHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
RS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA