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The map label for this gene is narK [H]

Identifier: 62390078

GI number: 62390078

Start: 1254022

End: 1255359

Strand: Reverse

Name: narK [H]

Synonym: cg1345

Alternate gene names: 62390078

Gene position: 1255359-1254022 (Counterclockwise)

Preceding gene: 62390080

Following gene: 62390077

Centisome position: 38.24

GC content: 52.99

Gene sequence:

>1338_bases
GTGACACAACTCAACACCAAAGGCGTTGTTCTGCAAGGGTGGGATCCAGAAGATCCTGAACATTGGGACTCGAAAATTGC
ATGGCGAACCCTGTGGATTACCACCTTCTCCATGATTATTGGGTTCTGCGTGTGGTATTTGGTTTCTGCCATCGCTCCCC
TACTCAATCGAATTGGATTTGATCTCTCAGCAGGTCAGCTTTATTGGCTCGCATCTATCCCCGGTTTGGCCGGCGGATTA
ATCCGATTGATTTACATGTTCCTTCCACCGATTCTTGGAACCCGCAAATTGGTCGGAATTTCCTCCGGTCTATTTTTGAT
CCCCATGTTTGGGTGGTTCCTGGCTGTCCAAGATTCAAGCACTCCCTACTGGTGGCTTCTCACACTCGCTGCACTCACTG
GCATTGGTGGTGGCGTGTTCTCTGGATATATGCCGTCCACGGGATACTTCTTCCCCAAGGCAAAATCGGGCACTGCGCTG
GGCATTCAGGCAGGTATCGGCAACCTCGGCGTCTCGATAATTCAGTTCATGGGCCCATGGGTCATGGGTTTCGGTCTGCT
GGGCATTGGTTTCCTCACCCCGCAGCGCACCATTGAAGGCACCACGGTGTTTGTGCACAATGCTGCGATTGTGTTGGTCC
CGTGGACTATTCTCGCGGCCGTTTTATCCTTCCTGTTTCTTAAAGATGTCCCAGTCACCGCAAATTTCCGGCAACAGATC
GATATCTTTGGCAACAAGAACACATGGATTTTGTCCATTATCTACTTGATGACATTCGGTGCCTTCGCCGGTTTCGCCGC
GCAGTTCGGTCTGATCATCAACAACAACTTCGGCATCGCTTCCCCGATGGCAGAGACTTATCCAGCTGAGATGCTTCACG
CCGGTGCTACGTTCGCGTTTCTTGGACCTTTGATTGGTGCTTTGGTGCGTGCTGCATGGGGTCCACTGTGTGACAGATTC
GGTGGAGCTATCTGGACCTTTGTCGGTGGCATCGGAATGACTATCGCCACTGCAGCTGCCGCAATCTTCCTAAGCAGAGC
GGAGACACCTGATGATTTCTGGCCATTCCTGTGGTCCATGCTTGCCCTGTTCTTCTTCACCGGTCTGGGCAATGCTGGCA
CCTTCAAACAAATGCCCATGATTTTGCCTAAACGCCAAGCAGGTGGCGTGATCGGCTGGACCGGTGCCATTGGTGCCTTC
GGCCCCTTCATTGTCGGTGTCTTGCTCTCCTTCACTCCAACTGTCGCGTTCTTCTGGGGCTGCGTGGTGTTCTTCATCAT
CGCCACCGCTTTGACCTGGATCTACTACGCCCGCCCGAACGCTCCATTCCCGGGATAA

Upstream 100 bases:

>100_bases
AACATGCGGGCGCAGGTCAGAGCTGTTATCTTAGTACTTATCACAGCCATAGGGCGGGCTTGACGGAAAGCCTTTCCGCG
TAACCATGAAGAGGCATCAC

Downstream 100 bases:

>100_bases
ACCGAAAGGCCAATCCATGACTACAACTACTTCTTCTGGGAAGTCTTCTGAAAAGATCAACCCCCTCTTCAAGCTCGGCA
GTTTCCTAAGAAAAGGCACC

Product: nitrate/nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTQLNTKGVVLQGWDPEDPEHWDSKIAWRTLWITTFSMIIGFCVWYLVSAIAPLLNRIGFDLSAGQLYWLASIPGLAGGL
IRLIYMFLPPILGTRKLVGISSGLFLIPMFGWFLAVQDSSTPYWWLLTLAALTGIGGGVFSGYMPSTGYFFPKAKSGTAL
GIQAGIGNLGVSIIQFMGPWVMGFGLLGIGFLTPQRTIEGTTVFVHNAAIVLVPWTILAAVLSFLFLKDVPVTANFRQQI
DIFGNKNTWILSIIYLMTFGAFAGFAAQFGLIINNNFGIASPMAETYPAEMLHAGATFAFLGPLIGALVRAAWGPLCDRF
GGAIWTFVGGIGMTIATAAAAIFLSRAETPDDFWPFLWSMLALFFFTGLGNAGTFKQMPMILPKRQAGGVIGWTGAIGAF
GPFIVGVLLSFTPTVAFFWGCVVFFIIATALTWIYYARPNAPFPG

Sequences:

>Translated_445_residues
MTQLNTKGVVLQGWDPEDPEHWDSKIAWRTLWITTFSMIIGFCVWYLVSAIAPLLNRIGFDLSAGQLYWLASIPGLAGGL
IRLIYMFLPPILGTRKLVGISSGLFLIPMFGWFLAVQDSSTPYWWLLTLAALTGIGGGVFSGYMPSTGYFFPKAKSGTAL
GIQAGIGNLGVSIIQFMGPWVMGFGLLGIGFLTPQRTIEGTTVFVHNAAIVLVPWTILAAVLSFLFLKDVPVTANFRQQI
DIFGNKNTWILSIIYLMTFGAFAGFAAQFGLIINNNFGIASPMAETYPAEMLHAGATFAFLGPLIGALVRAAWGPLCDRF
GGAIWTFVGGIGMTIATAAAAIFLSRAETPDDFWPFLWSMLALFFFTGLGNAGTFKQMPMILPKRQAGGVIGWTGAIGAF
GPFIVGVLLSFTPTVAFFWGCVVFFIIATALTWIYYARPNAPFPG
>Mature_444_residues
TQLNTKGVVLQGWDPEDPEHWDSKIAWRTLWITTFSMIIGFCVWYLVSAIAPLLNRIGFDLSAGQLYWLASIPGLAGGLI
RLIYMFLPPILGTRKLVGISSGLFLIPMFGWFLAVQDSSTPYWWLLTLAALTGIGGGVFSGYMPSTGYFFPKAKSGTALG
IQAGIGNLGVSIIQFMGPWVMGFGLLGIGFLTPQRTIEGTTVFVHNAAIVLVPWTILAAVLSFLFLKDVPVTANFRQQID
IFGNKNTWILSIIYLMTFGAFAGFAAQFGLIINNNFGIASPMAETYPAEMLHAGATFAFLGPLIGALVRAAWGPLCDRFG
GAIWTFVGGIGMTIATAAAAIFLSRAETPDDFWPFLWSMLALFFFTGLGNAGTFKQMPMILPKRQAGGVIGWTGAIGAFG
PFIVGVLLSFTPTVAFFWGCVVFFIIATALTWIYYARPNAPFPG

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=466, Percent_Identity=32.4034334763949, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787743, Length=471, Percent_Identity=32.6963906581741, Blast_Score=185, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48185; Mature: 48054

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLNTKGVVLQGWDPEDPEHWDSKIAWRTLWITTFSMIIGFCVWYLVSAIAPLLNRIGF
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLSAGQLYWLASIPGLAGGLIRLIYMFLPPILGTRKLVGISSGLFLIPMFGWFLAVQDSS
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHEEEECCC
TPYWWLLTLAALTGIGGGVFSGYMPSTGYFFPKAKSGTALGIQAGIGNLGVSIIQFMGPW
CCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
VMGFGLLGIGFLTPQRTIEGTTVFVHNAAIVLVPWTILAAVLSFLFLKDVPVTANFRQQI
HHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHE
DIFGNKNTWILSIIYLMTFGAFAGFAAQFGLIINNNFGIASPMAETYPAEMLHAGATFAF
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHCCHHHHHCCCHHHH
LGPLIGALVRAAWGPLCDRFGGAIWTFVGGIGMTIATAAAAIFLSRAETPDDFWPFLWSM
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LALFFFTGLGNAGTFKQMPMILPKRQAGGVIGWTGAIGAFGPFIVGVLLSFTPTVAFFWG
HHHHHHHCCCCCCCHHHCCCCCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
CVVFFIIATALTWIYYARPNAPFPG
HHHHHHHHHHHHHHHEECCCCCCCC
>Mature Secondary Structure 
TQLNTKGVVLQGWDPEDPEHWDSKIAWRTLWITTFSMIIGFCVWYLVSAIAPLLNRIGF
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLSAGQLYWLASIPGLAGGLIRLIYMFLPPILGTRKLVGISSGLFLIPMFGWFLAVQDSS
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHEEEECCC
TPYWWLLTLAALTGIGGGVFSGYMPSTGYFFPKAKSGTALGIQAGIGNLGVSIIQFMGPW
CCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
VMGFGLLGIGFLTPQRTIEGTTVFVHNAAIVLVPWTILAAVLSFLFLKDVPVTANFRQQI
HHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHE
DIFGNKNTWILSIIYLMTFGAFAGFAAQFGLIINNNFGIASPMAETYPAEMLHAGATFAF
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHCCHHHHHCCCHHHH
LGPLIGALVRAAWGPLCDRFGGAIWTFVGGIGMTIATAAAAIFLSRAETPDDFWPFLWSM
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LALFFFTGLGNAGTFKQMPMILPKRQAGGVIGWTGAIGAFGPFIVGVLLSFTPTVAFFWG
HHHHHHHCCCCCCCHHHCCCCCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
CVVFFIIATALTWIYYARPNAPFPG
HHHHHHHHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]