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The map label for this gene is yiaE [C]

Identifier: 62388965

GI number: 62388965

Start: 72815

End: 73729

Strand: Reverse

Name: yiaE [C]

Synonym: cg0091

Alternate gene names: 62388965

Gene position: 73729-72815 (Counterclockwise)

Preceding gene: 62388970

Following gene: 62388962

Centisome position: 2.25

GC content: 51.69

Gene sequence:

>915_bases
ATGAAAATCTTTGTTGGTTTTGGCGATTATCCACTCACCACCAAGGCCCTTAAGGAGGCAGGCGCAGAAATAGTGGACTC
CCTCGAAAAAGCCGAGGGGTTTGTCTTCACTCAAACACCAGGCACAGAATTTCCCCTACTTCCCGACGGAGTGAGGTGGG
TGCAATTTCCCAATGCGGGGCTCAACGCATATTTCACAGCTGGGCAGATTGATGACAAACGCCGGTGGTCAAATGCATCA
GGGGTGTATGGCCAACAGGTAGCCGAAGCAGCAATGGCTCTTTTGCTGGGACTGATTCATATGCACCCCACCATGGTGCG
TGCCGATAGTTGGGCACCAAGCACTCAAATAGATCAGCAGACCAGATGGCTTGATGGTGCAACAGTTGCCATTGTGGGAG
CTGGTGGAATCGGTAAACATCTGGCAGCCATGTTGAAACCTTTTGGCGCAAAGTCTTTAGCAGTAAGCAGGACCGGTACA
CCCACCCAAGATTTTGATGCAACGGAACCTATATCCAACCTGCACCAAGTACTTGCCGACGCCGACCATGTGGTGTTGTG
CGTACCGCTTACCGCAGACACCTATCATCTGATCGGAAAAGCAGAGCTTAAAGCAATGCAGTCCACTGCAATTTTGATCA
ACGTGGCTCGCGGAGAAGTAGTAGATACAGAAGCATTAGTTGACGCCTTAGATGCCCAAGAAATATCCGGGGCAGGCTTA
GATGTCACCGATCCTGAACCATTGCCGGACGATCATCCACTGTGGGGGCGCAGCAATGTGATCATTACCCCGCACGTAGC
CAACACGTTGACCTCGATGGATCGCATGCTTGCCCCAGTGGTGGCAGAAAACTACCGACGGTTTCTCGCTGGAGAAAAGA
TGCTGACCGAGGTGGACATCCACAAAGGTTACTAG

Upstream 100 bases:

>100_bases
AGAGATTGTTGATTCGGCACCAACTTTACCCACGAGGGTAGAAATGAAGCATTCAGGATGACAAAACCCAACCTCACACC
AACAACCTATCCTGGAGCCC

Downstream 100 bases:

>100_bases
AGCAGTGGCTTTGAATATAGATGTTCTGGTCGCCCGGACTGTCCGTACCGAAGGCGAACCTGGATCACACCTTGGCTAGC
CATCGCTGCCAGCCTGCGCT

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKIFVGFGDYPLTTKALKEAGAEIVDSLEKAEGFVFTQTPGTEFPLLPDGVRWVQFPNAGLNAYFTAGQIDDKRRWSNAS
GVYGQQVAEAAMALLLGLIHMHPTMVRADSWAPSTQIDQQTRWLDGATVAIVGAGGIGKHLAAMLKPFGAKSLAVSRTGT
PTQDFDATEPISNLHQVLADADHVVLCVPLTADTYHLIGKAELKAMQSTAILINVARGEVVDTEALVDALDAQEISGAGL
DVTDPEPLPDDHPLWGRSNVIITPHVANTLTSMDRMLAPVVAENYRRFLAGEKMLTEVDIHKGY

Sequences:

>Translated_304_residues
MKIFVGFGDYPLTTKALKEAGAEIVDSLEKAEGFVFTQTPGTEFPLLPDGVRWVQFPNAGLNAYFTAGQIDDKRRWSNAS
GVYGQQVAEAAMALLLGLIHMHPTMVRADSWAPSTQIDQQTRWLDGATVAIVGAGGIGKHLAAMLKPFGAKSLAVSRTGT
PTQDFDATEPISNLHQVLADADHVVLCVPLTADTYHLIGKAELKAMQSTAILINVARGEVVDTEALVDALDAQEISGAGL
DVTDPEPLPDDHPLWGRSNVIITPHVANTLTSMDRMLAPVVAENYRRFLAGEKMLTEVDIHKGY
>Mature_304_residues
MKIFVGFGDYPLTTKALKEAGAEIVDSLEKAEGFVFTQTPGTEFPLLPDGVRWVQFPNAGLNAYFTAGQIDDKRRWSNAS
GVYGQQVAEAAMALLLGLIHMHPTMVRADSWAPSTQIDQQTRWLDGATVAIVGAGGIGKHLAAMLKPFGAKSLAVSRTGT
PTQDFDATEPISNLHQVLADADHVVLCVPLTADTYHLIGKAELKAMQSTAILINVARGEVVDTEALVDALDAQEISGAGL
DVTDPEPLPDDHPLWGRSNVIITPHVANTLTSMDRMLAPVVAENYRRFLAGEKMLTEVDIHKGY

Specific function: Unknown

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=231, Percent_Identity=32.9004329004329, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4557497, Length=146, Percent_Identity=34.9315068493151, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI61743967, Length=171, Percent_Identity=32.1637426900585, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI145580578, Length=230, Percent_Identity=27.8260869565217, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4557499, Length=230, Percent_Identity=27.8260869565217, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI145580575, Length=184, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI23308577, Length=173, Percent_Identity=27.1676300578035, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI87082289, Length=224, Percent_Identity=33.0357142857143, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081824, Length=227, Percent_Identity=28.1938325991189, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787645, Length=283, Percent_Identity=26.1484098939929, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789279, Length=154, Percent_Identity=33.1168831168831, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=222, Percent_Identity=30.1801801801802, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324055, Length=173, Percent_Identity=32.3699421965318, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322116, Length=181, Percent_Identity=29.2817679558011, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320925, Length=158, Percent_Identity=32.2784810126582, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324980, Length=163, Percent_Identity=29.4478527607362, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=28.4313725490196, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325144, Length=156, Percent_Identity=31.4102564102564, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28571528, Length=192, Percent_Identity=35.9375, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI28574284, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585514, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574282, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45552429, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45551003, Length=227, Percent_Identity=33.4801762114537, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574286, Length=176, Percent_Identity=36.9318181818182, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585516, Length=223, Percent_Identity=27.8026905829596, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19921140, Length=184, Percent_Identity=27.1739130434783, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24646446, Length=237, Percent_Identity=29.535864978903, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646448, Length=237, Percent_Identity=29.535864978903, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646452, Length=237, Percent_Identity=29.535864978903, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646450, Length=237, Percent_Identity=29.535864978903, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI62472511, Length=238, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 32742; Mature: 32742

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFVGFGDYPLTTKALKEAGAEIVDSLEKAEGFVFTQTPGTEFPLLPDGVRWVQFPNAG
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEECCCCC
LNAYFTAGQIDDKRRWSNASGVYGQQVAEAAMALLLGLIHMHPTMVRADSWAPSTQIDQQ
CCEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHH
TRWLDGATVAIVGAGGIGKHLAAMLKPFGAKSLAVSRTGTPTQDFDATEPISNLHQVLAD
HHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHC
ADHVVLCVPLTADTYHLIGKAELKAMQSTAILINVARGEVVDTEALVDALDAQEISGAGL
CCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEEEECCCCEECHHHHHHHHHHHHHCCCCC
DVTDPEPLPDDHPLWGRSNVIITPHVANTLTSMDRMLAPVVAENYRRFLAGEKMLTEVDI
CCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEC
HKGY
CCCC
>Mature Secondary Structure
MKIFVGFGDYPLTTKALKEAGAEIVDSLEKAEGFVFTQTPGTEFPLLPDGVRWVQFPNAG
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEECCCCC
LNAYFTAGQIDDKRRWSNASGVYGQQVAEAAMALLLGLIHMHPTMVRADSWAPSTQIDQQ
CCEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHH
TRWLDGATVAIVGAGGIGKHLAAMLKPFGAKSLAVSRTGTPTQDFDATEPISNLHQVLAD
HHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHC
ADHVVLCVPLTADTYHLIGKAELKAMQSTAILINVARGEVVDTEALVDALDAQEISGAGL
CCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEEEECCCCEECHHHHHHHHHHHHHCCCCC
DVTDPEPLPDDHPLWGRSNVIITPHVANTLTSMDRMLAPVVAENYRRFLAGEKMLTEVDI
CCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEC
HKGY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]