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The map label for this gene is ppnK

Identifier: 59712604

GI number: 59712604

Start: 2232511

End: 2233404

Strand: Direct

Name: ppnK

Synonym: VF_1997

Alternate gene names: 59712604

Gene position: 2232511-2233404 (Clockwise)

Preceding gene: 59712598

Following gene: 59712605

Centisome position: 77.05

GC content: 40.94

Gene sequence:

>894_bases
ATGATGGATATGAAAAGAGCCTTTAACGTTATTGCTATCATTGGTAAACCAAGAGATCCTAATGCGATAAAAACGCATTT
ATCGATTTATCACTGGCTTACTGATCAAAATTATACGGTCCTTCTCGATGACCGTTTACGTGAAGCCCTACCTGAAATTC
CAGCTGACCGTTTCAATCATCTATTAAAGTTAGGGGAATTAGCTGATCTTGCCATTGTTGTTGGTGGTGATGGCAATATG
CTAGGTGCTGCACGTGTACTCTCTCGATTTGATATTTCAGTCATTGGGGTTAATAGAGGTAATCTTGGTTTCTTAACTGA
TCTTGATCCAGATAACTTTGAAGAACCTCTGCAAGCGGTATTAAATGGTGACTTTGTAAAAGAAGAGCGCTTTTTATTAG
AAGCTGAAGTTCATCGCCATGGGCAAGTTAAAAGCCATAATTCAGCCTTTAATGAAGTGGTACTTCACCCAGGCCAAGTC
GCACACATGATTGAATTTGAAGTGTATATTGATGACACTTTTGCTTTCTCTCAACGCTCAGATGGTTTGATTATCTCAAC
GCCTACAGGATCAACGGCCTATTCACTCTCTGGTGGCGGCCCGATTCTATCTCCAAACTTAAATGCCATCTCAATTGTAC
CAATGTTTCCACATACACTTTCAAGTCGCCCCTTGGTGGTCGAAGGAAAGCGTCACATTAAGTTGTGCATATCTCCAGAG
AACCGCACCACGTTAGAAGTAAGCTGTGATGGGCAAGTCTCTCTGCCTGTTAGTCCGGGTGATGAAGTACATATTTTCCA
AAGCCCATCAAGACTAAAGCTGATTCATCCTAAAGATTACAGCTACTATCATATACTTAGAAATAAGCTAGGGTGGTCAA
GTAAGTTGTTCTAA

Upstream 100 bases:

>100_bases
CTATACGCAAATTAAAACTTTGACATTATTATGGGGACTAAATAGTGCGATTCAAGCATAATAGTACGAATTGTAACATT
TGTTCCCCATTTCATGATGG

Downstream 100 bases:

>100_bases
CGAGTAAAGTTAAGTTAGTCACAAAAAATTTAAATTCACTTTACTGTATAAAGAAACAGTATATACTGTGTCTATATACA
GTGTTTTTCGTATTTTAAAT

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MMDMKRAFNVIAIIGKPRDPNAIKTHLSIYHWLTDQNYTVLLDDRLREALPEIPADRFNHLLKLGELADLAIVVGGDGNM
LGAARVLSRFDISVIGVNRGNLGFLTDLDPDNFEEPLQAVLNGDFVKEERFLLEAEVHRHGQVKSHNSAFNEVVLHPGQV
AHMIEFEVYIDDTFAFSQRSDGLIISTPTGSTAYSLSGGGPILSPNLNAISIVPMFPHTLSSRPLVVEGKRHIKLCISPE
NRTTLEVSCDGQVSLPVSPGDEVHIFQSPSRLKLIHPKDYSYYHILRNKLGWSSKLF

Sequences:

>Translated_297_residues
MMDMKRAFNVIAIIGKPRDPNAIKTHLSIYHWLTDQNYTVLLDDRLREALPEIPADRFNHLLKLGELADLAIVVGGDGNM
LGAARVLSRFDISVIGVNRGNLGFLTDLDPDNFEEPLQAVLNGDFVKEERFLLEAEVHRHGQVKSHNSAFNEVVLHPGQV
AHMIEFEVYIDDTFAFSQRSDGLIISTPTGSTAYSLSGGGPILSPNLNAISIVPMFPHTLSSRPLVVEGKRHIKLCISPE
NRTTLEVSCDGQVSLPVSPGDEVHIFQSPSRLKLIHPKDYSYYHILRNKLGWSSKLF
>Mature_297_residues
MMDMKRAFNVIAIIGKPRDPNAIKTHLSIYHWLTDQNYTVLLDDRLREALPEIPADRFNHLLKLGELADLAIVVGGDGNM
LGAARVLSRFDISVIGVNRGNLGFLTDLDPDNFEEPLQAVLNGDFVKEERFLLEAEVHRHGQVKSHNSAFNEVVLHPGQV
AHMIEFEVYIDDTFAFSQRSDGLIISTPTGSTAYSLSGGGPILSPNLNAISIVPMFPHTLSSRPLVVEGKRHIKLCISPE
NRTTLEVSCDGQVSLPVSPGDEVHIFQSPSRLKLIHPKDYSYYHILRNKLGWSSKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=325, Percent_Identity=28, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1788968, Length=294, Percent_Identity=58.843537414966, Blast_Score=357, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6320794, Length=242, Percent_Identity=31.404958677686, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=29.5833333333333, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325068, Length=210, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28573826, Length=317, Percent_Identity=29.0220820189274, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28573830, Length=318, Percent_Identity=28.9308176100629, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28573828, Length=318, Percent_Identity=28.9308176100629, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161077047, Length=318, Percent_Identity=28.9308176100629, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28573832, Length=318, Percent_Identity=28.9308176100629, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI20129957, Length=277, Percent_Identity=27.7978339350181, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281363321, Length=277, Percent_Identity=27.7978339350181, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24653424, Length=277, Percent_Identity=27.7978339350181, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24653422, Length=277, Percent_Identity=27.7978339350181, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281363323, Length=277, Percent_Identity=27.7978339350181, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_VIBF1 (Q5E3A4)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205380.1
- ProteinModelPortal:   Q5E3A4
- SMR:   Q5E3A4
- STRING:   Q5E3A4
- GeneID:   3279125
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1997
- NMPDR:   fig|312309.3.peg.1997
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   FESFMID
- PhylomeDB:   Q5E3A4
- ProtClustDB:   PRK03378
- BioCyc:   VFIS312309:VF1997-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 33071; Mature: 33071

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDMKRAFNVIAIIGKPRDPNAIKTHLSIYHWLTDQNYTVLLDDRLREALPEIPADRFNH
CCCHHHHCEEEEEEECCCCCCHHHHEEEEEEEECCCCCEEEECHHHHHHHCCCCHHHHHH
LLKLGELADLAIVVGGDGNMLGAARVLSRFDISVIGVNRGNLGFLTDLDPDNFEEPLQAV
HHHHCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCHHHHHHHH
LNGDFVKEERFLLEAEVHRHGQVKSHNSAFNEVVLHPGQVAHMIEFEVYIDDTFAFSQRS
HCCCCCCHHHHEEEEHHHHCCCCCCCCCCCHHEEECCCCEEEEEEEEEEEECEEEECCCC
DGLIISTPTGSTAYSLSGGGPILSPNLNAISIVPMFPHTLSSRPLVVEGKRHIKLCISPE
CCEEEECCCCCEEEEECCCCCEECCCCCEEEEEEECCCCCCCCCEEEECCCEEEEEECCC
NRTTLEVSCDGQVSLPVSPGDEVHIFQSPSRLKLIHPKDYSYYHILRNKLGWSSKLF
CCEEEEEEECCEEEEECCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MMDMKRAFNVIAIIGKPRDPNAIKTHLSIYHWLTDQNYTVLLDDRLREALPEIPADRFNH
CCCHHHHCEEEEEEECCCCCCHHHHEEEEEEEECCCCCEEEECHHHHHHHCCCCHHHHHH
LLKLGELADLAIVVGGDGNMLGAARVLSRFDISVIGVNRGNLGFLTDLDPDNFEEPLQAV
HHHHCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCHHHHHHHH
LNGDFVKEERFLLEAEVHRHGQVKSHNSAFNEVVLHPGQVAHMIEFEVYIDDTFAFSQRS
HCCCCCCHHHHEEEEHHHHCCCCCCCCCCCHHEEECCCCEEEEEEEEEEEECEEEECCCC
DGLIISTPTGSTAYSLSGGGPILSPNLNAISIVPMFPHTLSSRPLVVEGKRHIKLCISPE
CCEEEECCCCCEEEEECCCCCEECCCCCEEEEEEECCCCCCCCCEEEECCCEEEEEECCC
NRTTLEVSCDGQVSLPVSPGDEVHIFQSPSRLKLIHPKDYSYYHILRNKLGWSSKLF
CCEEEEEEECCEEEEECCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA