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The map label for this gene is map [H]

Identifier: 59712570

GI number: 59712570

Start: 2193082

End: 2193909

Strand: Direct

Name: map [H]

Synonym: VF_1963

Alternate gene names: 59712570

Gene position: 2193082-2193909 (Clockwise)

Preceding gene: 59712547

Following gene: 59712571

Centisome position: 75.69

GC content: 38.04

Gene sequence:

>828_bases
ATGTCAGTAAAGATTAAAAACGCTGCAGAAATTGAAAAAATGCGCGTAGCCGGCCAATTAGCCGCTGAAGTACTTGAAAT
GATTGAACCTTATGTGAAAGCTGGGGTAACAACAGAAGAACTTGATCAAATTTGCCATAAGCACATTACTGAAGTTCAAG
GCGCAATCCCTGCTCCATTAAATTACCATGGTTTCCCTAAATCTATCTGTACATCAATTAACCATATTGTTTGTCATGGT
ATTCCTGCATCACAAGATGAGCAATACAATGGTTTTGTAAAACCTGCAGTATTAAAAGATGGTGACATCCTTAATATTGA
TATCACAGTAATCAAAGACGGTTACCACGGTGATACTTCTAAGATGTTCTTAGTTGGTGATGTAAGCCCAACAGATAAAC
GTCTGTGTCTAATTGCACAAGAGAGTTTATATGTTGGTATGCGTAAAGTTAAACCGGGTGTAAAACTGGGTGAAATTGGT
ACTGCTATTCAAAAATACATCAAAGGTACAACACCAAAATGTTCTATCGTACGTGATTACTGTGGGCATGGTATTGGTTC
TGAATTCCATGAAGAACCACAAGTTGTTCATTACAAAAACAGCGATCGTACTGTATTAAAAGCAGGCATGACATTTACTA
TTGAACCAATGATCAATTTAGGTAAATTCGGTTGTCTTCAAGACGACAACGATGGTTGGACAGTATACACAGGCGACGGT
AAAAATACCGCTCAATGGGAACACACTATTTTAGTAACCGAAACGGGTTGTGAAGTGTTAACTTTACGTGCTGAAGAAAC
CATTCCTCGCCACATGAACAACAAATAA

Upstream 100 bases:

>100_bases
AGGTATTCCCATCACCTTATAATTGAAAGATACAGGCCGCCTAGGTAAACTTAGATACATTATCGTTTAATACGATTTAA
TTTTAGATAGAGAAAAGCCC

Downstream 100 bases:

>100_bases
CAAATCGCAAATAAACAGAAGGCTTCGGTCTTCTGTTTTTTTATCAGCAAGGATTGCTATGAAATATCTATCCCCCCTAT
CCTTATCCGATACTCAGCTT

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSVKIKNAAEIEKMRVAGQLAAEVLEMIEPYVKAGVTTEELDQICHKHITEVQGAIPAPLNYHGFPKSICTSINHIVCHG
IPASQDEQYNGFVKPAVLKDGDILNIDITVIKDGYHGDTSKMFLVGDVSPTDKRLCLIAQESLYVGMRKVKPGVKLGEIG
TAIQKYIKGTTPKCSIVRDYCGHGIGSEFHEEPQVVHYKNSDRTVLKAGMTFTIEPMINLGKFGCLQDDNDGWTVYTGDG
KNTAQWEHTILVTETGCEVLTLRAEETIPRHMNNK

Sequences:

>Translated_275_residues
MSVKIKNAAEIEKMRVAGQLAAEVLEMIEPYVKAGVTTEELDQICHKHITEVQGAIPAPLNYHGFPKSICTSINHIVCHG
IPASQDEQYNGFVKPAVLKDGDILNIDITVIKDGYHGDTSKMFLVGDVSPTDKRLCLIAQESLYVGMRKVKPGVKLGEIG
TAIQKYIKGTTPKCSIVRDYCGHGIGSEFHEEPQVVHYKNSDRTVLKAGMTFTIEPMINLGKFGCLQDDNDGWTVYTGDG
KNTAQWEHTILVTETGCEVLTLRAEETIPRHMNNK
>Mature_274_residues
SVKIKNAAEIEKMRVAGQLAAEVLEMIEPYVKAGVTTEELDQICHKHITEVQGAIPAPLNYHGFPKSICTSINHIVCHGI
PASQDEQYNGFVKPAVLKDGDILNIDITVIKDGYHGDTSKMFLVGDVSPTDKRLCLIAQESLYVGMRKVKPGVKLGEIGT
AIQKYIKGTTPKCSIVRDYCGHGIGSEFHEEPQVVHYKNSDRTVLKAGMTFTIEPMINLGKFGCLQDDNDGWTVYTGDGK
NTAQWEHTILVTETGCEVLTLRAEETIPRHMNNK

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=275, Percent_Identity=44.3636363636364, Blast_Score=226, Evalue=1e-59,
Organism=Homo sapiens, GI40385867, Length=268, Percent_Identity=39.1791044776119, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI1786364, Length=270, Percent_Identity=59.2592592592593, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1788728, Length=266, Percent_Identity=24.812030075188, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71996291, Length=265, Percent_Identity=44.1509433962264, Blast_Score=207, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6323273, Length=268, Percent_Identity=42.5373134328358, Blast_Score=215, Evalue=7e-57,
Organism=Drosophila melanogaster, GI21355531, Length=266, Percent_Identity=45.8646616541353, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24583427, Length=262, Percent_Identity=44.2748091603053, Blast_Score=206, Evalue=1e-53,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 30356; Mature: 30225

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKIKNAAEIEKMRVAGQLAAEVLEMIEPYVKAGVTTEELDQICHKHITEVQGAIPAPL
CCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
NYHGFPKSICTSINHIVCHGIPASQDEQYNGFVKPAVLKDGDILNIDITVIKDGYHGDTS
CCCCCCHHHHHHHHHEEEECCCCCCCCCCCCEECCEEECCCCEEEEEEEEEECCCCCCCC
KMFLVGDVSPTDKRLCLIAQESLYVGMRKVKPGVKLGEIGTAIQKYIKGTTPKCSIVRDY
CEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHH
CGHGIGSEFHEEPQVVHYKNSDRTVLKAGMTFTIEPMINLGKFGCLQDDNDGWTVYTGDG
HCCCCCCHHHCCCCEEEEECCCCEEEEECCEEEEECCCCCCCCCCEEECCCCEEEEECCC
KNTAQWEHTILVTETGCEVLTLRAEETIPRHMNNK
CCCCCEEEEEEEEECCCEEEEEECHHHCCHHCCCC
>Mature Secondary Structure 
SVKIKNAAEIEKMRVAGQLAAEVLEMIEPYVKAGVTTEELDQICHKHITEVQGAIPAPL
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
NYHGFPKSICTSINHIVCHGIPASQDEQYNGFVKPAVLKDGDILNIDITVIKDGYHGDTS
CCCCCCHHHHHHHHHEEEECCCCCCCCCCCCEECCEEECCCCEEEEEEEEEECCCCCCCC
KMFLVGDVSPTDKRLCLIAQESLYVGMRKVKPGVKLGEIGTAIQKYIKGTTPKCSIVRDY
CEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHH
CGHGIGSEFHEEPQVVHYKNSDRTVLKAGMTFTIEPMINLGKFGCLQDDNDGWTVYTGDG
HCCCCCCHHHCCCCEEEEECCCCEEEEECCEEEEECCCCCCCCCCEEECCCCEEEEECCC
KNTAQWEHTILVTETGCEVLTLRAEETIPRHMNNK
CCCCCEEEEEEEEECCCEEEEEECHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]