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The map label for this gene is pilJ [H]

Identifier: 59711479

GI number: 59711479

Start: 956359

End: 957447

Strand: Reverse

Name: pilJ [H]

Synonym: VF_0872

Alternate gene names: 59711479

Gene position: 957447-956359 (Counterclockwise)

Preceding gene: 59711481

Following gene: 59711478

Centisome position: 33.04

GC content: 39.39

Gene sequence:

>1089_bases
ATGTTTAGATTAAATCGAGTCAAAGCAGAAAATGAAGCTCTAAAAAAAGAGTTATCTGACCTTAAAAATAAATACCAAAC
AGATATTCAGAAATTAGAAGATCAGCTCCAAGAAGCTAAACAGCAAGTCATCTCAGTACAGCAAGATTATCAAAGTAGCG
ACGGTTTGATTTCGAGCAGTTTAAAAGGTGGTGATATGCTTCAAACCATTCGAACTGCAATGGTAGAAAGCGCTCAATCG
ATGGCGCAAGAGAATGAAAACCTAGAACAACTTAGCGAAGTCTTTAAACAAACCCATCAAGCCCTCACTCGACTTGACAA
TAGAGCCGTAGAAATAAGCTCTCAAACAACCCACAGCATTAAATCTGTTCAAATATTAGATGAAACGGCTAACTCCATTT
CTCATCTTGTCTCTACTATCCAAGAAATTTCTGACCAAACAAACTTACTTGCTCTCAATGCAGCAATTGAAGCAGCTCGT
GCAGGTGATGCTGGCCGAGGATTTGCTGTTGTTGCTGATGAAGTAAGAACCCTAGCAAGCAAAGCCAGTGACGCTAGCGA
GCAGATTGATTCCCTAGTGAACCAAGTGTTAACTCAAGTGGATGCCATTAAAATATCTATCGATGAAAACCAAGCCTGTG
CAGAAGAAGTATCAGCCTCTTCAGCACAAATTGGAACCATCGTAAATGAGGTAGTTGATAAATCAGAGCACATGAAAGAG
ATCATTTATGTCGCTTCAACGAGAGCGTTCCTTGATACGGTAAAACTTGATCATGCGATTTGGAAAAACAATGTGTATCG
CTTACTGCAAAAACACTCATTCGACGAAACCGTTAATACTCACTCTGAATGTCGATTGGGTCAGTGGTACTACAAAGGAA
GTGGCAAAGCTTATAATCATCTAAAAAGCTATGCTCTACTGGAAGAGCCTCACAAGGCAGTTCACGACAATGGACGTAAA
GCCATGAGTAAAGCTCAAACAGGTAATATGGCTGGTATGATTACATCAATTAATGCAATGGAAGATTCAAGCGAACAAGT
CGTAATTCAAATTGATCGTTTAATGGATGAAATCGTTGCAGATAAATAA

Upstream 100 bases:

>100_bases
AAATATTTCACTTAATTAACCAATATCTATTACCTTTGGTATGACAAAAATCAACCATTCTAGGTAGACTCGGGAAATCA
TTTAATTAATTTCTTAGCTT

Downstream 100 bases:

>100_bases
AATAATTTATCTCAATTCGATTGTGGGCTTGAGTGAATTATCAAGCCCATCCCACCACTAAAAAGTATTCACATCATTTC
GTGATGCGAATAACCCAGCT

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MFRLNRVKAENEALKKELSDLKNKYQTDIQKLEDQLQEAKQQVISVQQDYQSSDGLISSSLKGGDMLQTIRTAMVESAQS
MAQENENLEQLSEVFKQTHQALTRLDNRAVEISSQTTHSIKSVQILDETANSISHLVSTIQEISDQTNLLALNAAIEAAR
AGDAGRGFAVVADEVRTLASKASDASEQIDSLVNQVLTQVDAIKISIDENQACAEEVSASSAQIGTIVNEVVDKSEHMKE
IIYVASTRAFLDTVKLDHAIWKNNVYRLLQKHSFDETVNTHSECRLGQWYYKGSGKAYNHLKSYALLEEPHKAVHDNGRK
AMSKAQTGNMAGMITSINAMEDSSEQVVIQIDRLMDEIVADK

Sequences:

>Translated_362_residues
MFRLNRVKAENEALKKELSDLKNKYQTDIQKLEDQLQEAKQQVISVQQDYQSSDGLISSSLKGGDMLQTIRTAMVESAQS
MAQENENLEQLSEVFKQTHQALTRLDNRAVEISSQTTHSIKSVQILDETANSISHLVSTIQEISDQTNLLALNAAIEAAR
AGDAGRGFAVVADEVRTLASKASDASEQIDSLVNQVLTQVDAIKISIDENQACAEEVSASSAQIGTIVNEVVDKSEHMKE
IIYVASTRAFLDTVKLDHAIWKNNVYRLLQKHSFDETVNTHSECRLGQWYYKGSGKAYNHLKSYALLEEPHKAVHDNGRK
AMSKAQTGNMAGMITSINAMEDSSEQVVIQIDRLMDEIVADK
>Mature_362_residues
MFRLNRVKAENEALKKELSDLKNKYQTDIQKLEDQLQEAKQQVISVQQDYQSSDGLISSSLKGGDMLQTIRTAMVESAQS
MAQENENLEQLSEVFKQTHQALTRLDNRAVEISSQTTHSIKSVQILDETANSISHLVSTIQEISDQTNLLALNAAIEAAR
AGDAGRGFAVVADEVRTLASKASDASEQIDSLVNQVLTQVDAIKISIDENQACAEEVSASSAQIGTIVNEVVDKSEHMKE
IIYVASTRAFLDTVKLDHAIWKNNVYRLLQKHSFDETVNTHSECRLGQWYYKGSGKAYNHLKSYALLEEPHKAVHDNGRK
AMSKAQTGNMAGMITSINAMEDSSEQVVIQIDRLMDEIVADK

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=173, Percent_Identity=35.2601156069364, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788195, Length=170, Percent_Identity=32.9411764705882, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788194, Length=157, Percent_Identity=35.031847133758, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367378, Length=165, Percent_Identity=32.7272727272727, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789453, Length=135, Percent_Identity=37.037037037037, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 40144; Mature: 40144

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLNRVKAENEALKKELSDLKNKYQTDIQKLEDQLQEAKQQVISVQQDYQSSDGLISSS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
LKGGDMLQTIRTAMVESAQSMAQENENLEQLSEVFKQTHQALTRLDNRAVEISSQTTHSI
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
KSVQILDETANSISHLVSTIQEISDQTNLLALNAAIEAARAGDAGRGFAVVADEVRTLAS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KASDASEQIDSLVNQVLTQVDAIKISIDENQACAEEVSASSAQIGTIVNEVVDKSEHMKE
HCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IIYVASTRAFLDTVKLDHAIWKNNVYRLLQKHSFDETVNTHSECRLGQWYYKGSGKAYNH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHCCCCCEEECCCCHHHHH
LKSYALLEEPHKAVHDNGRKAMSKAQTGNMAGMITSINAMEDSSEQVVIQIDRLMDEIVA
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
DK
CC
>Mature Secondary Structure
MFRLNRVKAENEALKKELSDLKNKYQTDIQKLEDQLQEAKQQVISVQQDYQSSDGLISSS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
LKGGDMLQTIRTAMVESAQSMAQENENLEQLSEVFKQTHQALTRLDNRAVEISSQTTHSI
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
KSVQILDETANSISHLVSTIQEISDQTNLLALNAAIEAARAGDAGRGFAVVADEVRTLAS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KASDASEQIDSLVNQVLTQVDAIKISIDENQACAEEVSASSAQIGTIVNEVVDKSEHMKE
HCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IIYVASTRAFLDTVKLDHAIWKNNVYRLLQKHSFDETVNTHSECRLGQWYYKGSGKAYNH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHCCCCCEEECCCCHHHHH
LKSYALLEEPHKAVHDNGRKAMSKAQTGNMAGMITSINAMEDSSEQVVIQIDRLMDEIVA
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
DK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]