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The map label for this gene is ackA

Identifier: 59711444

GI number: 59711444

Start: 923664

End: 924860

Strand: Reverse

Name: ackA

Synonym: VF_0837

Alternate gene names: 59711444

Gene position: 924860-923664 (Counterclockwise)

Preceding gene: 59711446

Following gene: 59711443

Centisome position: 31.92

GC content: 44.78

Gene sequence:

>1197_bases
ATGTCTAAGCTGGTTTTAGTTTTAAACTGCGGTAGTTCTTCTCTTAAATTTGCGGTTGTTGACGCAGAATCTGGTGCAGA
GCACTTAACTGGTCTTGCTGAGTGTCTTGGTCTTCCTGAAGCTCGTATGAAGTGGAAACTTGATGGCAAGCACGAAGCTC
AACTAGGTGCAGGCGCTGCACACGTAGAAGCTCTATCATTTATGGTAGAAACTATCCTTGCATCTAAGCCAGAGCTTAAA
GCTAACCTTGGCGCTATCGGTCACCGTATCGTACACGGTGGTGAGCAATTCACTAAGTCAGCTCTTATCACTGACGAAGT
ACTTAAAGGTATTCAAGACGCTGCAACTTTTGCACCTCTTCATAACCCAGCTCACATCATCGGTATCGAAGCAGCTAAAG
CTAACTTCCCAGGCCTTAAAAACGTTGCTGTATTTGACACTGCGTTCCACCAAACTATGCCTGAAGAATCTTACCTATAC
GCTCTTCCATACAACCTATACAAAGAGCACGGCATCCGTCGTTACGGCATGCACGGTACTTCTCACCTGTTCATCACTCG
TGAAGTTGCTGGCCTACTAAACAAACCTGTTGAAGAAGTTAACATCATTAACTGTCACCTAGGTAACGGCGCATCTGTAT
GTGCAATTAAAAACGGTGAATCTGTAGATACTTCTATGGGTCTTACTCCTCTTGAAGGTCTAGTAATGGGTACTCGTTGT
GGTGACATCGATCCTGCTATCGTTTTCCACCTACATGACGCTCTAGGTTACTCTGTTGAACAAATCAACAACATGCTAAC
TAAAGAGTCTGGCCTACAAGGTCTAACTCAAGTAACTTCTGACTGTCGTTTCGTTGAAGACAACTACGGTGAGAAAGAAG
AAGCAACACGTGCGATGGACGTATTCTGTCACCGTCTAGCTAAATACGTTGCAGGTTACACAGCAACTCTAGAAGGTCGT
CTAGACGCAATCACTTTCACAGGCGGCATCGGTGAAAACTCTGCTCCAATCCGTGAAATGGTTCTTAACCGTCTAGGTAT
CTTCGGTATCGAAGTTGATGGCGAAGCAAACCTTAAAGCACGTTTCGGTGGTGAAGGTACTATCACTACTGCTAACAGCC
GTATTCCTGCAATGGTTATCTCTACTAACGAAGAGCTAGTTATCGCAGAAGACACAGCTAAACTAGCTGGTCTGTAA

Upstream 100 bases:

>100_bases
TTTGGATAAATGTGGTTGTTTTCAATTAATCGACTACATTTGTCGTGATATGTCATTACGACATAACTTTAATTACTGAT
GAATAAATAGGTATTTATAC

Downstream 100 bases:

>100_bases
TATCTCCTAAAAGGCTAGCCATTAGGTTAGCCTTTTTTCTTTCTATGGCTTTATTTTTTCAATAAGGTCTCTTGCCCCAA
TAGTGTAGAGGAATTTTCCA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 1

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSKLVLVLNCGSSSLKFAVVDAESGAEHLTGLAECLGLPEARMKWKLDGKHEAQLGAGAAHVEALSFMVETILASKPELK
ANLGAIGHRIVHGGEQFTKSALITDEVLKGIQDAATFAPLHNPAHIIGIEAAKANFPGLKNVAVFDTAFHQTMPEESYLY
ALPYNLYKEHGIRRYGMHGTSHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGESVDTSMGLTPLEGLVMGTRC
GDIDPAIVFHLHDALGYSVEQINNMLTKESGLQGLTQVTSDCRFVEDNYGEKEEATRAMDVFCHRLAKYVAGYTATLEGR
LDAITFTGGIGENSAPIREMVLNRLGIFGIEVDGEANLKARFGGEGTITTANSRIPAMVISTNEELVIAEDTAKLAGL

Sequences:

>Translated_398_residues
MSKLVLVLNCGSSSLKFAVVDAESGAEHLTGLAECLGLPEARMKWKLDGKHEAQLGAGAAHVEALSFMVETILASKPELK
ANLGAIGHRIVHGGEQFTKSALITDEVLKGIQDAATFAPLHNPAHIIGIEAAKANFPGLKNVAVFDTAFHQTMPEESYLY
ALPYNLYKEHGIRRYGMHGTSHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGESVDTSMGLTPLEGLVMGTRC
GDIDPAIVFHLHDALGYSVEQINNMLTKESGLQGLTQVTSDCRFVEDNYGEKEEATRAMDVFCHRLAKYVAGYTATLEGR
LDAITFTGGIGENSAPIREMVLNRLGIFGIEVDGEANLKARFGGEGTITTANSRIPAMVISTNEELVIAEDTAKLAGL
>Mature_397_residues
SKLVLVLNCGSSSLKFAVVDAESGAEHLTGLAECLGLPEARMKWKLDGKHEAQLGAGAAHVEALSFMVETILASKPELKA
NLGAIGHRIVHGGEQFTKSALITDEVLKGIQDAATFAPLHNPAHIIGIEAAKANFPGLKNVAVFDTAFHQTMPEESYLYA
LPYNLYKEHGIRRYGMHGTSHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGESVDTSMGLTPLEGLVMGTRCG
DIDPAIVFHLHDALGYSVEQINNMLTKESGLQGLTQVTSDCRFVEDNYGEKEEATRAMDVFCHRLAKYVAGYTATLEGRL
DAITFTGGIGENSAPIREMVLNRLGIFGIEVDGEANLKARFGGEGTITTANSRIPAMVISTNEELVIAEDTAKLAGL

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=72.544080604534, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI145693184, Length=395, Percent_Identity=45.0632911392405, Blast_Score=341, Evalue=4e-95,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA1_VIBF1 (Q5E6L4)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204220.1
- ProteinModelPortal:   Q5E6L4
- SMR:   Q5E6L4
- STRING:   Q5E6L4
- GeneID:   3278383
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0837
- NMPDR:   fig|312309.3.peg.649
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   PHLKDKN
- PhylomeDB:   Q5E6L4
- ProtClustDB:   PRK00180
- BioCyc:   VFIS312309:VF0837-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 42661; Mature: 42530

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVLVLNCGSSSLKFAVVDAESGAEHLTGLAECLGLPEARMKWKLDGKHEAQLGAGAA
CCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHEEEEECCCCCHHHCCCHH
HVEALSFMVETILASKPELKANLGAIGHRIVHGGEQFTKSALITDEVLKGIQDAATFAPL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HNPAHIIGIEAAKANFPGLKNVAVFDTAFHQTMPEESYLYALPYNLYKEHGIRRYGMHGT
CCCCEEEEEEECCCCCCCCCCEEEEEHHHHHCCCCCCEEEEECHHHHHHCCCEEECCCCC
SHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGESVDTSMGLTPLEGLVMGTRC
CEEEEHHHHHHHHHCCHHHEEEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHEEEECCC
GDIDPAIVFHLHDALGYSVEQINNMLTKESGLQGLTQVTSDCRFVEDNYGEKEEATRAMD
CCCCCEEEEEEHHHHCCCHHHHHHHHHHHHCHHHHHHHHHCCEEECCCCCCHHHHHHHHH
VFCHRLAKYVAGYTATLEGRLDAITFTGGIGENSAPIREMVLNRLGIFGIEVDGEANLKA
HHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEE
RFGGEGTITTANSRIPAMVISTNEELVIAEDTAKLAGL
EECCCEEEEECCCCCCEEEEECCCCEEEEECCHHHCCC
>Mature Secondary Structure 
SKLVLVLNCGSSSLKFAVVDAESGAEHLTGLAECLGLPEARMKWKLDGKHEAQLGAGAA
CCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHEEEEECCCCCHHHCCCHH
HVEALSFMVETILASKPELKANLGAIGHRIVHGGEQFTKSALITDEVLKGIQDAATFAPL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HNPAHIIGIEAAKANFPGLKNVAVFDTAFHQTMPEESYLYALPYNLYKEHGIRRYGMHGT
CCCCEEEEEEECCCCCCCCCCEEEEEHHHHHCCCCCCEEEEECHHHHHHCCCEEECCCCC
SHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGESVDTSMGLTPLEGLVMGTRC
CEEEEHHHHHHHHHCCHHHEEEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHEEEECCC
GDIDPAIVFHLHDALGYSVEQINNMLTKESGLQGLTQVTSDCRFVEDNYGEKEEATRAMD
CCCCCEEEEEEHHHHCCCHHHHHHHHHHHHCHHHHHHHHHCCEEECCCCCCHHHHHHHHH
VFCHRLAKYVAGYTATLEGRLDAITFTGGIGENSAPIREMVLNRLGIFGIEVDGEANLKA
HHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEE
RFGGEGTITTANSRIPAMVISTNEELVIAEDTAKLAGL
EECCCEEEEECCCCCCEEEEECCCCEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA