Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 59711411

GI number: 59711411

Start: 881139

End: 882806

Strand: Reverse

Name: asnB [H]

Synonym: VF_0804

Alternate gene names: 59711411

Gene position: 882806-881139 (Counterclockwise)

Preceding gene: 59711412

Following gene: 59711409

Centisome position: 30.47

GC content: 39.45

Gene sequence:

>1668_bases
ATGTGCTCAATTTTCGGAATTCTAGATATTAAAAGCGATCCAACTCCACTAAGAACTACCGCTTTAGAAATGTCTAAAAA
GTTACGCCATCGAGGTCCAGACTGGTCAGGTATCTATTCATCTGACAAAGCAATTTTAGCGCATGAACGTTTAGCTATCG
TTGGTTTAAACAGTGGCGCACAACCTCTTTATAGCGAAGATAAAAAGCACATTCTTGCTGTTAATGGTGAAATTTATAAC
CACAAAGAGCTTCGCGAAAAATACGCAGATAATTACACATTCCAAACTGATTCAGATTGTGAAGTTATTTTAGCGTTATA
TCGTGAAAAAGGAGCTGATCTACTCGAAGATTTAAATGGTATTTTTGCCTTCATTCTTTACGATGAAGAAAAAGATGAAT
ATTTAATTGGTCGTGACCATATTGGTATTATCCCTATGTATCACGGTTATGATGAACATGGTAACTATTATGTTGCTTCA
GAAATGAAAGCATTAGTTCCTGTATGTAAATCAATCAGTGAGTTTCCTCCTGGCCACTTCTACTCATCAAAAGATACAGA
ACCAACACGTTATTATTTACGTGATTGGATGGATTATGATGCAGTTAAAGATAACCCAACAAGTAAAGAAGACTTAACCC
AAGCACTTGAAGATGCTGTAAAACGCCAATTAATGACAGATGTTCCTTATGGTGTACTTCTTTCTGGTGGATTAGATTCA
TCTATTACGTCTGCCGTAGCTAAACGTTTCGCAGCGATGCGTATTGAAGACGATGAAAAGTCGGAAGCATGGTGGCCTCA
ACTGCACTCTTTTGCTGTTGGCTTAGAAGGCGCACCAGATCTAAAAGCCGCTCGTGAAGTTGCAGATAAACTGGGTACGG
TTCACCATGAAATGACTTACACCATTCAAGAAGGTTTGGATGCGATTCGTGATGTTATTTATCATATTGAAACCTATGAT
GTAACCACTATTCGAGCATCAACACCAATGTTCTTAATGGGTCGTAAAATCAAAGCGATGGGCATCAAGATGGTACTTTC
TGGTGAAGGTGCAGATGAAATCTTTGGTGGCTACCTCTACTTCCATAAAGCACCAAACAAAAAAGAGTTCCATGAAGAGA
CCGTTCGTAAACTACTTGCTTTAAACATGTTTGATTGTGCTCGTGCGAATAAATCACTTGCAGCTTGGGGGGTTGAAGGT
CGTGTTCCATTCTTAGATAAAGAATTTATTGATGTGGCTATGCGTTTAAACCCACAAGATAAGATGTGTGGTAATGGAAA
GATGGAAAAACACATCTTACGTGAATGTTTTGAACATTATTTACCTGAAGCTATCGCATGGAGACAGAAAGAACAATTCT
CTGATGGTGTTGGATATAGCTGGATCGACACATTACGAGAAACCGCAGAGGCAAAAGTAACAGACCAACAAATGGAATCA
GCAAAATTCCGCTTCCCGTATAACACGCCAACAACAAAAGAAGCGTATGTTTATCGAGAAATCTTTGAAGAGTTATTCCC
GCTAGAAGATGCAGCGAAATGTGTTCCTGGTGGTCCGTCAGTTGCTTGCTCATCAGCTAAAGCAATTGAATGGGATGAAT
CTTTCCAGAACTGCGTAGATCCGTCAGGTCGTGCTGTTCAAGCCGTTCATAACGAAGCATACGATTAA

Upstream 100 bases:

>100_bases
TTATTTGTATTTCCGGGTTGATTTTTTTAGCGGTAATGGCAAATTGTATTCAGGACAATAATTTAAGACAAAAATTAAGA
AGAGTAGAAGGAACTTATTT

Downstream 100 bases:

>100_bases
TGCACTAGCACCATTTTAATGTAATAAAAAAGCGCCTTATAGGCGCTTTTTTTATGCATCAATATTCGATATCTGAGTTA
GGTATTTTAATTTCTACTTT

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MCSIFGILDIKSDPTPLRTTALEMSKKLRHRGPDWSGIYSSDKAILAHERLAIVGLNSGAQPLYSEDKKHILAVNGEIYN
HKELREKYADNYTFQTDSDCEVILALYREKGADLLEDLNGIFAFILYDEEKDEYLIGRDHIGIIPMYHGYDEHGNYYVAS
EMKALVPVCKSISEFPPGHFYSSKDTEPTRYYLRDWMDYDAVKDNPTSKEDLTQALEDAVKRQLMTDVPYGVLLSGGLDS
SITSAVAKRFAAMRIEDDEKSEAWWPQLHSFAVGLEGAPDLKAAREVADKLGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNKKEFHEETVRKLLALNMFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPQDKMCGNGKMEKHILRECFEHYLPEAIAWRQKEQFSDGVGYSWIDTLRETAEAKVTDQQMES
AKFRFPYNTPTTKEAYVYREIFEELFPLEDAAKCVPGGPSVACSSAKAIEWDESFQNCVDPSGRAVQAVHNEAYD

Sequences:

>Translated_555_residues
MCSIFGILDIKSDPTPLRTTALEMSKKLRHRGPDWSGIYSSDKAILAHERLAIVGLNSGAQPLYSEDKKHILAVNGEIYN
HKELREKYADNYTFQTDSDCEVILALYREKGADLLEDLNGIFAFILYDEEKDEYLIGRDHIGIIPMYHGYDEHGNYYVAS
EMKALVPVCKSISEFPPGHFYSSKDTEPTRYYLRDWMDYDAVKDNPTSKEDLTQALEDAVKRQLMTDVPYGVLLSGGLDS
SITSAVAKRFAAMRIEDDEKSEAWWPQLHSFAVGLEGAPDLKAAREVADKLGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNKKEFHEETVRKLLALNMFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPQDKMCGNGKMEKHILRECFEHYLPEAIAWRQKEQFSDGVGYSWIDTLRETAEAKVTDQQMES
AKFRFPYNTPTTKEAYVYREIFEELFPLEDAAKCVPGGPSVACSSAKAIEWDESFQNCVDPSGRAVQAVHNEAYD
>Mature_555_residues
MCSIFGILDIKSDPTPLRTTALEMSKKLRHRGPDWSGIYSSDKAILAHERLAIVGLNSGAQPLYSEDKKHILAVNGEIYN
HKELREKYADNYTFQTDSDCEVILALYREKGADLLEDLNGIFAFILYDEEKDEYLIGRDHIGIIPMYHGYDEHGNYYVAS
EMKALVPVCKSISEFPPGHFYSSKDTEPTRYYLRDWMDYDAVKDNPTSKEDLTQALEDAVKRQLMTDVPYGVLLSGGLDS
SITSAVAKRFAAMRIEDDEKSEAWWPQLHSFAVGLEGAPDLKAAREVADKLGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNKKEFHEETVRKLLALNMFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPQDKMCGNGKMEKHILRECFEHYLPEAIAWRQKEQFSDGVGYSWIDTLRETAEAKVTDQQMES
AKFRFPYNTPTTKEAYVYREIFEELFPLEDAAKCVPGGPSVACSSAKAIEWDESFQNCVDPSGRAVQAVHNEAYD

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=543, Percent_Identity=38.121546961326, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI168229252, Length=543, Percent_Identity=38.121546961326, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI168229250, Length=543, Percent_Identity=38.121546961326, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI296010848, Length=556, Percent_Identity=37.410071942446, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI296010852, Length=487, Percent_Identity=38.1930184804928, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI296010850, Length=487, Percent_Identity=38.1930184804928, Blast_Score=290, Evalue=3e-78,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=76.5342960288809, Blast_Score=917, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=571, Percent_Identity=43.4325744308231, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71993933, Length=559, Percent_Identity=43.8282647584973, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17560178, Length=521, Percent_Identity=36.084452975048, Blast_Score=298, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI25147560, Length=341, Percent_Identity=36.3636363636364, Blast_Score=200, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=52.4770642201835, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6325403, Length=580, Percent_Identity=49.4827586206897, Blast_Score=542, Evalue=1e-155,
Organism=Drosophila melanogaster, GI45553209, Length=580, Percent_Identity=46.8965517241379, Blast_Score=487, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62945; Mature: 62945

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGILDIKSDPTPLRTTALEMSKKLRHRGPDWSGIYSSDKAILAHERLAIVGLNSGA
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCC
QPLYSEDKKHILAVNGEIYNHKELREKYADNYTFQTDSDCEVILALYREKGADLLEDLNG
CCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCC
IFAFILYDEEKDEYLIGRDHIGIIPMYHGYDEHGNYYVASEMKALVPVCKSISEFPPGHF
EEEEEEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
YSSKDTEPTRYYLRDWMDYDAVKDNPTSKEDLTQALEDAVKRQLMTDVPYGVLLSGGLDS
CCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SITSAVAKRFAAMRIEDDEKSEAWWPQLHSFAVGLEGAPDLKAAREVADKLGTVHHEMTY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TIQEGLDAIRDVIYHIETYDVTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLY
HHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHCCEEHCCCEEEEEECCCHHHHHCCEEE
FHKAPNKKEFHEETVRKLLALNMFDCARANKSLAAWGVEGRVPFLDKEFIDVAMRLNPQD
EECCCCHHHHHHHHHHHHHHHCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCC
KMCGNGKMEKHILRECFEHYLPEAIAWRQKEQFSDGVGYSWIDTLRETAEAKVTDQQMES
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AKFRFPYNTPTTKEAYVYREIFEELFPLEDAAKCVPGGPSVACSSAKAIEWDESFQNCVD
CCEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCC
PSGRAVQAVHNEAYD
CCCCHHHHHHHCCCC
>Mature Secondary Structure
MCSIFGILDIKSDPTPLRTTALEMSKKLRHRGPDWSGIYSSDKAILAHERLAIVGLNSGA
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCC
QPLYSEDKKHILAVNGEIYNHKELREKYADNYTFQTDSDCEVILALYREKGADLLEDLNG
CCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCC
IFAFILYDEEKDEYLIGRDHIGIIPMYHGYDEHGNYYVASEMKALVPVCKSISEFPPGHF
EEEEEEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
YSSKDTEPTRYYLRDWMDYDAVKDNPTSKEDLTQALEDAVKRQLMTDVPYGVLLSGGLDS
CCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SITSAVAKRFAAMRIEDDEKSEAWWPQLHSFAVGLEGAPDLKAAREVADKLGTVHHEMTY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TIQEGLDAIRDVIYHIETYDVTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLY
HHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHCCEEHCCCEEEEEECCCHHHHHCCEEE
FHKAPNKKEFHEETVRKLLALNMFDCARANKSLAAWGVEGRVPFLDKEFIDVAMRLNPQD
EECCCCHHHHHHHHHHHHHHHCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCC
KMCGNGKMEKHILRECFEHYLPEAIAWRQKEQFSDGVGYSWIDTLRETAEAKVTDQQMES
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AKFRFPYNTPTTKEAYVYREIFEELFPLEDAAKCVPGGPSVACSSAKAIEWDESFQNCVD
CCEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCC
PSGRAVQAVHNEAYD
CCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]