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The map label for this gene is lipB

Identifier: 59711350

GI number: 59711350

Start: 810828

End: 811505

Strand: Reverse

Name: lipB

Synonym: VF_0743

Alternate gene names: 59711350

Gene position: 811505-810828 (Counterclockwise)

Preceding gene: 59711351

Following gene: 59711349

Centisome position: 28.01

GC content: 37.02

Gene sequence:

>678_bases
TTGAACAAACGTCTAGTGGTTAAAAATCTTGGTCGACAAGATTACGAACCGGTATGGAAAGCAATGCATACTTTTACCGA
TGAACGTGATGAAAATACATGTGATGAAGTATGGCTAGTGGAACATAACCCTGTATTTACACAAGGACAAGCAGGCAAAG
AAGAACACGTTCTTGCTGCTGGTGATATCCCTATTGTAAAAAGCGATAGAGGTGGACAAGTGACTTATCACGGTCCGGGT
CAATTAGTTGCTTATGTATTAATTAATTTGCGACGTAAAAACATTGGTGTACGTGAATTAGTAACGCATATTGAAAATAC
CGTAATCAATACATTAAGTCATTTTAATGTTGAGTCAGCAGCACGTCCTGATGCTCCGGGAGTGTATGTTGATAACAAGA
AAATCTGCTCATTAGGATTACGCATCCGTAAAGGATGTTCATTTCATGGATTAGCACTTAACATTAATATGGATTTATCT
CCTTTCCTACGTATAAACCCTTGTGGTTATGCTGGTATGGAAATGATCCAACTTCATGACTTAGTTAAAGATAACATGAC
AGTAGAGAATAAAGCAGAACACGTCGAAGATGTTCATCCTTTACTGATTAAAGAATTAACAACACTACTTGATTACACTG
ATATCGAGTATTTAACGGAAAGCAGCAATCATGAGTAA

Upstream 100 bases:

>100_bases
CTACCTTTTTGATCTTTTGATATACCCCTACATTTCTTATCTATTCCTTCCCTTCTATCCTTGCTATCATTAGGCAAATT
TTCAACCTAGTGTGATCTAA

Downstream 100 bases:

>100_bases
GCCAATCCAAATGGAAAAAGGTGTAAAATACCGCGATGCCGATAAAATGGCATTAATTCCAGTTAAAAACATGCCTACAG
AGCAAAAAGAAGTACTACGT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MNKRLVVKNLGRQDYEPVWKAMHTFTDERDENTCDEVWLVEHNPVFTQGQAGKEEHVLAAGDIPIVKSDRGGQVTYHGPG
QLVAYVLINLRRKNIGVRELVTHIENTVINTLSHFNVESAARPDAPGVYVDNKKICSLGLRIRKGCSFHGLALNINMDLS
PFLRINPCGYAGMEMIQLHDLVKDNMTVENKAEHVEDVHPLLIKELTTLLDYTDIEYLTESSNHE

Sequences:

>Translated_225_residues
MNKRLVVKNLGRQDYEPVWKAMHTFTDERDENTCDEVWLVEHNPVFTQGQAGKEEHVLAAGDIPIVKSDRGGQVTYHGPG
QLVAYVLINLRRKNIGVRELVTHIENTVINTLSHFNVESAARPDAPGVYVDNKKICSLGLRIRKGCSFHGLALNINMDLS
PFLRINPCGYAGMEMIQLHDLVKDNMTVENKAEHVEDVHPLLIKELTTLLDYTDIEYLTESSNHE
>Mature_225_residues
MNKRLVVKNLGRQDYEPVWKAMHTFTDERDENTCDEVWLVEHNPVFTQGQAGKEEHVLAAGDIPIVKSDRGGQVTYHGPG
QLVAYVLINLRRKNIGVRELVTHIENTVINTLSHFNVESAARPDAPGVYVDNKKICSLGLRIRKGCSFHGLALNINMDLS
PFLRINPCGYAGMEMIQLHDLVKDNMTVENKAEHVEDVHPLLIKELTTLLDYTDIEYLTESSNHE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=154, Percent_Identity=35.0649350649351, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI87081767, Length=216, Percent_Identity=61.5740740740741, Blast_Score=290, Evalue=6e-80,
Organism=Saccharomyces cerevisiae, GI6323268, Length=167, Percent_Identity=31.1377245508982, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355483, Length=159, Percent_Identity=32.0754716981132, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_VIBF1 (Q5E6V8)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204126.1
- ProteinModelPortal:   Q5E6V8
- SMR:   Q5E6V8
- STRING:   Q5E6V8
- GeneID:   3277873
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0743
- NMPDR:   fig|312309.3.peg.1244
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   Q5E6V8
- ProtClustDB:   PRK14342
- BioCyc:   VFIS312309:VF0743-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25441; Mature: 25441

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRLVVKNLGRQDYEPVWKAMHTFTDERDENTCDEVWLVEHNPVFTQGQAGKEEHVLAA
CCCEEEHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEECCCCCCCCCEEEE
GDIPIVKSDRGGQVTYHGPGQLVAYVLINLRRKNIGVRELVTHIENTVINTLSHFNVESA
CCCCEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
ARPDAPGVYVDNKKICSLGLRIRKGCSFHGLALNINMDLSPFLRINPCGYAGMEMIQLHD
CCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHH
LVKDNMTVENKAEHVEDVHPLLIKELTTLLDYTDIEYLTESSNHE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNKRLVVKNLGRQDYEPVWKAMHTFTDERDENTCDEVWLVEHNPVFTQGQAGKEEHVLAA
CCCEEEHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEECCCCCCCCCEEEE
GDIPIVKSDRGGQVTYHGPGQLVAYVLINLRRKNIGVRELVTHIENTVINTLSHFNVESA
CCCCEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
ARPDAPGVYVDNKKICSLGLRIRKGCSFHGLALNINMDLSPFLRINPCGYAGMEMIQLHD
CCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHH
LVKDNMTVENKAEHVEDVHPLLIKELTTLLDYTDIEYLTESSNHE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA