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The map label for this gene is lipA [H]

Identifier: 59711349

GI number: 59711349

Start: 809870

End: 810835

Strand: Reverse

Name: lipA [H]

Synonym: VF_0742

Alternate gene names: 59711349

Gene position: 810835-809870 (Counterclockwise)

Preceding gene: 59711350

Following gene: 59711343

Centisome position: 27.98

GC content: 42.44

Gene sequence:

>966_bases
ATGAGTAAGCCAATCCAAATGGAAAAAGGTGTAAAATACCGCGATGCCGATAAAATGGCATTAATTCCAGTTAAAAACAT
GCCTACAGAGCAAAAAGAAGTACTACGTAAGCCTGAATGGATGAAAATCAAACTTCCTGCTAGCAGTAAACGTATCGACG
ATATCAAGTCAGCAATGCGTAAGAATAACCTTCACTCGGTATGTGAAGAAGCATCTTGCCCTAACCTAGCTGAATGTTTT
AACCACGGTACTGCAACCTTCATGATCTTAGGTGCTATCTGTACCCGCCGTTGTCCTTTCTGTGATGTAGCCCATGGCCG
TCCTGTTGCACCAGAAGCAGAAGAACCAAAAAAACTGGCTAAAACAATTCAAGATATGAAATTGAAGTATGTTGTAATTA
CTTCGGTTGACCGTGATGATTTACGTGATGGTGGTGCTCAGCATTTTGCAGATTGTAACCGTGAAATCCGTGCATTAAAT
CCTGAAATCCGCATTGAAACATTGGTTCCAGATTTCCGTGGCCGTATGGATCGTGCTCTTGAAGCGATGATCGATAACCC
ACCAGATGTATTTAACCATAACTTAGAAACCGCGCCTCGTCTTTACCGTAAAGTACGCCCAGGAGCGAATTACCAATGGT
CTTTAGATCTACTTAAAAAGTTCAAAGAACAGCACCCGAACGTTCCAACTAAATCGGGCCTAATGATGGGCTTAGGTGAA
ACAAAAGAAGAGATTGTTGAAGTATTAAAAGACTTACGAGCTCATGGTGTAACCATGCTTACTCTGGGTCAATACTTAGC
ACCAAGTCGTCACCACTTACCAGTAGAGCGCTACGTTCCACCTGCTGAGTTTGATGAATTAAAAGAAATCGCATTAGAGC
TTGGATTCACTCACGCTGCATGTGGTCCTTTTGTTCGCTCTTCTTACCATGCCGATCTTCAAGCTAAAGGCGAAGAAGTG
AAGTAA

Upstream 100 bases:

>100_bases
AAAGCAGAACACGTCGAAGATGTTCATCCTTTACTGATTAAAGAATTAACAACACTACTTGATTACACTGATATCGAGTA
TTTAACGGAAAGCAGCAATC

Downstream 100 bases:

>100_bases
GGTCTAGGGTCTAGGGTCTAGGGTCTAGGGTCTAGGGTCTAGGGTCTAGGGTCTAGGGTCTGAATAATAAAAAAGGTTGA
CGTTTTGGCGTCAACCTTTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSKPIQMEKGVKYRDADKMALIPVKNMPTEQKEVLRKPEWMKIKLPASSKRIDDIKSAMRKNNLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPVAPEAEEPKKLAKTIQDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRALN
PEIRIETLVPDFRGRMDRALEAMIDNPPDVFNHNLETAPRLYRKVRPGANYQWSLDLLKKFKEQHPNVPTKSGLMMGLGE
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKEIALELGFTHAACGPFVRSSYHADLQAKGEEV
K

Sequences:

>Translated_321_residues
MSKPIQMEKGVKYRDADKMALIPVKNMPTEQKEVLRKPEWMKIKLPASSKRIDDIKSAMRKNNLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPVAPEAEEPKKLAKTIQDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRALN
PEIRIETLVPDFRGRMDRALEAMIDNPPDVFNHNLETAPRLYRKVRPGANYQWSLDLLKKFKEQHPNVPTKSGLMMGLGE
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKEIALELGFTHAACGPFVRSSYHADLQAKGEEV
K
>Mature_320_residues
SKPIQMEKGVKYRDADKMALIPVKNMPTEQKEVLRKPEWMKIKLPASSKRIDDIKSAMRKNNLHSVCEEASCPNLAECFN
HGTATFMILGAICTRRCPFCDVAHGRPVAPEAEEPKKLAKTIQDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRALNP
EIRIETLVPDFRGRMDRALEAMIDNPPDVFNHNLETAPRLYRKVRPGANYQWSLDLLKKFKEQHPNVPTKSGLMMGLGET
KEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKEIALELGFTHAACGPFVRSSYHADLQAKGEEVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=45.7337883959044, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI37577164, Length=257, Percent_Identity=45.5252918287938, Blast_Score=219, Evalue=2e-57,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=80.3738317757009, Blast_Score=552, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=42.5087108013937, Blast_Score=222, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=42.9042904290429, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=46.1805555555556, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36395; Mature: 36263

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIQMEKGVKYRDADKMALIPVKNMPTEQKEVLRKPEWMKIKLPASSKRIDDIKSAMR
CCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
KNNLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPEAEEPKKLA
HCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
KTIQDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRALNPEIRIETLVPDFRGRMDRAL
HHHHHCCEEEEEEECCCHHHHHCCCHHHHHHHCHHHHHCCCCEEEEEECCHHHHHHHHHH
EAMIDNPPDVFNHNLETAPRLYRKVRPGANYQWSLDLLKKFKEQHPNVPTKSGLMMGLGE
HHHHCCCHHHHCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCCC
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKEIALELGFTHAA
CHHHHHHHHHHHHHCCEEHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCHHHH
CGPFVRSSYHADLQAKGEEVK
HHHHHHHCCCCCHHCCCCCCC
>Mature Secondary Structure 
SKPIQMEKGVKYRDADKMALIPVKNMPTEQKEVLRKPEWMKIKLPASSKRIDDIKSAMR
CCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
KNNLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPEAEEPKKLA
HCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
KTIQDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRALNPEIRIETLVPDFRGRMDRAL
HHHHHCCEEEEEEECCCHHHHHCCCHHHHHHHCHHHHHCCCCEEEEEECCHHHHHHHHHH
EAMIDNPPDVFNHNLETAPRLYRKVRPGANYQWSLDLLKKFKEQHPNVPTKSGLMMGLGE
HHHHCCCHHHHCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCCC
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKEIALELGFTHAA
CHHHHHHHHHHHHHCCEEHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCHHHH
CGPFVRSSYHADLQAKGEEVK
HHHHHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA