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The map label for this gene is codB [H]

Identifier: 59711139

GI number: 59711139

Start: 569999

End: 571240

Strand: Reverse

Name: codB [H]

Synonym: VF_0532

Alternate gene names: 59711139

Gene position: 571240-569999 (Counterclockwise)

Preceding gene: 59711140

Following gene: 59711138

Centisome position: 19.71

GC content: 41.79

Gene sequence:

>1242_bases
ATGTCTCACGACAATGATTACAGCTTAGGGCCAGTACCTAAGACAGCCAGAAAAGGCGTCGTTTCCTTAACTTTAGTTAT
GTTAGGACTCACATTTTTCTCGGCAAGTATGTGGACAGGCGGTTCACTTGGGACCGGACTTTCTTTTAATGATTTCTTCC
TCGCTGTTCTTATTGGTAATTTAATCCTCGGCATTTACACTTCTTTTCTTGGTTACATCGGCGCTTCAACTGGTCTCTCT
ACTCACCTCCTCGCTCGTTTTTCTTTTGGCTCTAAAGGCTCTTGGCTTCCCTCTCTACTTCTCGGTGGTACTCAAGTTGG
TTGGTTTGGTGTTGGTGTTGCAATGTTTGCTATCCCAGTGCAAAAAGCCACAGGCATAGATACCAACGCTCTTATTGTTA
TCTCTGGTCTATTAATGACCGTCACTGTTTACTTCGGTATATCTGCTTTAATGGTTTTATCTATGGTTGCAGTGCCTGCG
ATTGCCATTTTAGGTGGATTCTCAGTATTTAGTGCTGTTGATAGTATTGGTGGTATTGCTGAGCTTCAAAAGATTCAACC
TGCAGAACCTATGGACTTTTCGGTTGCTCTTGCCATGGTTGTTGGTTCTTTTGTAAGTGCCGGAACATTAACAGCTGACT
TCGTCCGCTTTGGCAAAAAACCAATGGGTGCAGTTGCTATCACAATGATTGCTTTCTTTATCGGTAACTCTTTAATGTTC
ATCTTTGGTGCCGCTGGCGCTTCTGTTACTGGTCAGTCTGATATTTCAGAAGTGATGATTGCACAAGGGCTATTAATACC
TGCAATTATCGTATTAGGCCTAAATATTTGGACGACAAACGACAATGCGCTATACGCGTCTGGGCTTGGTTTTTCAAATA
TTACTGGCTTACCGAGTAAATATGTTTCTATGCTAAATGGTCTAATTGGTACTGTTTGTGCACTTTGGCTATACAATAAC
TTTGTAGGTTGGTTAACGTTCTTATCTTTAGCGATTCCACCTATTGGTGGTGTTATCATTGCGGACTTCTTTAGTAACCG
TAAACGCTACATGAATTTTGATAACGCAGCATTCCAAACCATTAACTGGGCAGGTATTATCGCAGTAGCCATTGGTGTTG
CCGCTGGTAAATTCTTACCGGGCGTTGTTCCTGTAAACGCTGTATTAGGCGGAGCGATTAGCTACCTATTACTAAACCCA
ATGATTAACAAAAAATCGATCTCTGAAACAGAAACAGTATAG

Upstream 100 bases:

>100_bases
ACAAAGATTACATTATTGACAATAATATAATTTTCTTTAGACTCTCGTCCGTTTGTCAAAAACTTGACCAAAATACATTC
AATACAGACGAGGATTGCAT

Downstream 100 bases:

>100_bases
AAGATACAGCAATGACAAATTTATTAATAAAAAACGTAGCTCTACGAGATCAAGAAGGTTTATTTCAGATCTTGATTGAA
GATGGTCAATTCAAAACCAT

Product: cytosine permease

Products: Proton [Cytoplasm]; cytosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSHDNDYSLGPVPKTARKGVVSLTLVMLGLTFFSASMWTGGSLGTGLSFNDFFLAVLIGNLILGIYTSFLGYIGASTGLS
THLLARFSFGSKGSWLPSLLLGGTQVGWFGVGVAMFAIPVQKATGIDTNALIVISGLLMTVTVYFGISALMVLSMVAVPA
IAILGGFSVFSAVDSIGGIAELQKIQPAEPMDFSVALAMVVGSFVSAGTLTADFVRFGKKPMGAVAITMIAFFIGNSLMF
IFGAAGASVTGQSDISEVMIAQGLLIPAIIVLGLNIWTTNDNALYASGLGFSNITGLPSKYVSMLNGLIGTVCALWLYNN
FVGWLTFLSLAIPPIGGVIIADFFSNRKRYMNFDNAAFQTINWAGIIAVAIGVAAGKFLPGVVPVNAVLGGAISYLLLNP
MINKKSISETETV

Sequences:

>Translated_413_residues
MSHDNDYSLGPVPKTARKGVVSLTLVMLGLTFFSASMWTGGSLGTGLSFNDFFLAVLIGNLILGIYTSFLGYIGASTGLS
THLLARFSFGSKGSWLPSLLLGGTQVGWFGVGVAMFAIPVQKATGIDTNALIVISGLLMTVTVYFGISALMVLSMVAVPA
IAILGGFSVFSAVDSIGGIAELQKIQPAEPMDFSVALAMVVGSFVSAGTLTADFVRFGKKPMGAVAITMIAFFIGNSLMF
IFGAAGASVTGQSDISEVMIAQGLLIPAIIVLGLNIWTTNDNALYASGLGFSNITGLPSKYVSMLNGLIGTVCALWLYNN
FVGWLTFLSLAIPPIGGVIIADFFSNRKRYMNFDNAAFQTINWAGIIAVAIGVAAGKFLPGVVPVNAVLGGAISYLLLNP
MINKKSISETETV
>Mature_412_residues
SHDNDYSLGPVPKTARKGVVSLTLVMLGLTFFSASMWTGGSLGTGLSFNDFFLAVLIGNLILGIYTSFLGYIGASTGLST
HLLARFSFGSKGSWLPSLLLGGTQVGWFGVGVAMFAIPVQKATGIDTNALIVISGLLMTVTVYFGISALMVLSMVAVPAI
AILGGFSVFSAVDSIGGIAELQKIQPAEPMDFSVALAMVVGSFVSAGTLTADFVRFGKKPMGAVAITMIAFFIGNSLMFI
FGAAGASVTGQSDISEVMIAQGLLIPAIIVLGLNIWTTNDNALYASGLGFSNITGLPSKYVSMLNGLIGTVCALWLYNNF
VGWLTFLSLAIPPIGGVIIADFFSNRKRYMNFDNAAFQTINWAGIIAVAIGVAAGKFLPGVVPVNAVLGGAISYLLLNPM
INKKSISETETV

Specific function: Required for cytosine transport into the cell [H]

COG id: COG1457

COG function: function code F; Purine-cytosine permease and related proteins

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI1786530, Length=413, Percent_Identity=74.09200968523, Blast_Score=610, Evalue=1e-176,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 43074; Mature: 42943

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure 
SHDNDYSLGPVPKTARKGVVSLTLVMLGLTFFSASMWTGGSLGTGLSFNDFFLAVLIGN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LILGIYTSFLGYIGASTGLSTHLLARFSFGSKGSWLPSLLLGGTQVGWFGVGVAMFAIPV
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
QKATGIDTNALIVISGLLMTVTVYFGISALMVLSMVAVPAIAILGGFSVFSAVDSIGGIA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELQKIQPAEPMDFSVALAMVVGSFVSAGTLTADFVRFGKKPMGAVAITMIAFFIGNSLMF
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEE
IFGAAGASVTGQSDISEVMIAQGLLIPAIIVLGLNIWTTNDNALYASGLGFSNITGLPSK
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCCCCCCHH
YVSMLNGLIGTVCALWLYNNFVGWLTFLSLAIPPIGGVIIADFFSNRKRYMNFDNAAFQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCHHHHH
INWAGIIAVAIGVAAGKFLPGVVPVNAVLGGAISYLLLNPMINKKSISETETV
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; cytosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + cytosine [Periplasm] = Proton [Cytoplasm] + cytosine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]