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The map label for this gene is proS [H]

Identifier: 172087674

GI number: 172087674

Start: 744514

End: 746241

Strand: Reverse

Name: proS [H]

Synonym: VF_0682

Alternate gene names: 172087674

Gene position: 746241-744514 (Counterclockwise)

Preceding gene: 59711290

Following gene: 59711288

Centisome position: 25.75

GC content: 43.63

Gene sequence:

>1728_bases
ATGGATGAAAACATGCGCACTAGCAAATACCTTCTTTCAACACTGAAGGAGACTCCAAACGACGCAGAAGTAGTAAGCCA
CCAGCTTATGCTACGTGCAGGTATGATTCGTAGACTGGCTTCAGGTTTATATACTTGGCTACCTACTGGTCTACGTGTAC
TGCGTAAAGTCGAAAACATCGTTCGTCAAGAGATCGACAATGCAGGTGCAATCGAAACATTGATGCCCGTAGTTCAACCA
TTTGAACTTTGGGAAGAAACAGGCCGCTCAGAAAAAATGGGCCCTGAACTACTTCGTTTTACTGACCGTCATGTTCGTCC
GTTTGTACTTAGTCCAACAGCTGAAGAAGTTATCACTAGCCTTGTTCGTAACGAAGTTAGCTCTTACAAACAACTTCCGC
TAAACCTGTACCAAATTCAAACTAAATTCCGTGACGAACGTCGTCCACGTTTTGGCGTAATGCGTGCTCGTGAATTCTGC
ATGATGGATGCATACAGCTTTGACATCAACAAAGAAGGCCTACAAAAGTCTTACGATGCAATGCACGATGCATACTGTAA
AGCATTCGACCGTATGGGTCTTGAATACCGTCCAGTACTAGCTGACTCTGGTGCTATCGGTGGTAGTGGTTCTCAAGAGT
TCCACGTACTTGCAGAAAGCGGTGAAGATTTAATCGCATTCTCAACAGAATCTGATTACGCAGCAAACATCGAAAAAGCA
GAAGCAGTAGCACCAACAACAGAACGTGCAGCTCCTACTCAAGAGATGACAACCGTTGATACACCAAACGCAAAAACGAT
TGCAGAATTAGTTGAGCAACACGGTATTGCGATTGAAAAAACAGTAAAAACACTGTTTGTTAAAGCGTCTGATGAAGTTG
ATGCTGATATTATCGCACTTATCATCCGTGGTGATCACGAGCTTAACGAAGTTAAAGCTGAAAACCTTCCACAGGTTGCT
TCTCCACTAGAAATGGCTGACGAAGCACAACTTCGTGACCTAATTGGTGCAGGCGCAGGTTCTCTTGGTCCTGTTGGCCT
AGAACTTCCATTCATCGTTGACCGTTCAGTTGCTGTAATGAGTGACTTTGGCACAGGTGCAAACATCGATGGTAAACACT
TCTTTGGTGTGAACTGGGATCGTGATGTACAACTAGGTCAAGTTGAAGATTTACGTAACGTAGTTGAAGGTGATCCAAGC
CCATGTGGTAAAGGTACGCTACAACTTAAACGTGGTATCGAAGTTGGTCACATCTTCCAATTAGGTACAGCCTACTCAGA
AGCAATGAACTGTGGCGTTCTAGATGCAAACGGTAAGAACGTAATTCTAGAAATGGGTTGTTACGGTATCGGTGTATCAC
GTGTTGTTGCATCAGCTATTGAACAAAACAACGACGAATACGGCATTGTATGGCCAGAAGCGATTGCACCATTTACGGTT
GCTATCGTTCCTATGAACATGCACAAATCTGAGCGTGTTAAAGAAGCAGCTGAAAAACTGTATGCTGAATTAACAGCAAT
GGGTATCGAAGTTCTATTCGATGACCGTAAAGAACGCCCAGGTGTTATGTTTAAGGACATCGAGCTTATCGGTATTCCAC
ACACGATCGTTATCGGTGATCGTAGCATGGACGAAGGTCACTTTGAGTATAAGAACCGTCGTGCAAATACGAAAGAAGCG
GTTGAAATGGCAAACATTGTTGAACACATTAAAGCGCAATTAAGCTAA

Upstream 100 bases:

>100_bases
TTAGATTTGAGCCCATTCTTAAGAGAACAGACTCTATACTAAGGTTGAACTCTCGGTTTAACTGTTTAAATCGAGCTATT
CCAATATTCATAACTAACAA

Downstream 100 bases:

>100_bases
TTTAATCACGTATTAAATACCAAAGGCTAAGTAAATTTACTTAGCCTTTTTTATATCTATAAAATAATTTTAGTTTCATT
GATTTATTTCTTGATTACTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MDENMRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRRLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQP
FELWEETGRSEKMGPELLRFTDRHVRPFVLSPTAEEVITSLVRNEVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFC
MMDAYSFDINKEGLQKSYDAMHDAYCKAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSTESDYAANIEKA
EAVAPTTERAAPTQEMTTVDTPNAKTIAELVEQHGIAIEKTVKTLFVKASDEVDADIIALIIRGDHELNEVKAENLPQVA
SPLEMADEAQLRDLIGAGAGSLGPVGLELPFIVDRSVAVMSDFGTGANIDGKHFFGVNWDRDVQLGQVEDLRNVVEGDPS
PCGKGTLQLKRGIEVGHIFQLGTAYSEAMNCGVLDANGKNVILEMGCYGIGVSRVVASAIEQNNDEYGIVWPEAIAPFTV
AIVPMNMHKSERVKEAAEKLYAELTAMGIEVLFDDRKERPGVMFKDIELIGIPHTIVIGDRSMDEGHFEYKNRRANTKEA
VEMANIVEHIKAQLS

Sequences:

>Translated_575_residues
MDENMRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRRLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQP
FELWEETGRSEKMGPELLRFTDRHVRPFVLSPTAEEVITSLVRNEVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFC
MMDAYSFDINKEGLQKSYDAMHDAYCKAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSTESDYAANIEKA
EAVAPTTERAAPTQEMTTVDTPNAKTIAELVEQHGIAIEKTVKTLFVKASDEVDADIIALIIRGDHELNEVKAENLPQVA
SPLEMADEAQLRDLIGAGAGSLGPVGLELPFIVDRSVAVMSDFGTGANIDGKHFFGVNWDRDVQLGQVEDLRNVVEGDPS
PCGKGTLQLKRGIEVGHIFQLGTAYSEAMNCGVLDANGKNVILEMGCYGIGVSRVVASAIEQNNDEYGIVWPEAIAPFTV
AIVPMNMHKSERVKEAAEKLYAELTAMGIEVLFDDRKERPGVMFKDIELIGIPHTIVIGDRSMDEGHFEYKNRRANTKEA
VEMANIVEHIKAQLS
>Mature_575_residues
MDENMRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRRLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQP
FELWEETGRSEKMGPELLRFTDRHVRPFVLSPTAEEVITSLVRNEVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFC
MMDAYSFDINKEGLQKSYDAMHDAYCKAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSTESDYAANIEKA
EAVAPTTERAAPTQEMTTVDTPNAKTIAELVEQHGIAIEKTVKTLFVKASDEVDADIIALIIRGDHELNEVKAENLPQVA
SPLEMADEAQLRDLIGAGAGSLGPVGLELPFIVDRSVAVMSDFGTGANIDGKHFFGVNWDRDVQLGQVEDLRNVVEGDPS
PCGKGTLQLKRGIEVGHIFQLGTAYSEAMNCGVLDANGKNVILEMGCYGIGVSRVVASAIEQNNDEYGIVWPEAIAPFTV
AIVPMNMHKSERVKEAAEKLYAELTAMGIEVLFDDRKERPGVMFKDIELIGIPHTIVIGDRSMDEGHFEYKNRRANTKEA
VEMANIVEHIKAQLS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=237, Percent_Identity=42.1940928270042, Blast_Score=202, Evalue=6e-52,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=70.3508771929825, Blast_Score=837, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=265, Percent_Identity=36.9811320754717, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI193203271, Length=99, Percent_Identity=38.3838383838384, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=552, Percent_Identity=31.3405797101449, Blast_Score=239, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24656200, Length=225, Percent_Identity=35.5555555555556, Blast_Score=155, Evalue=1e-37,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63772; Mature: 63772

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDENMRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRRLASGLYTWLPTGLRVLRKVENI
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRQEIDNAGAIETLMPVVQPFELWEETGRSEKMGPELLRFTDRHVRPFVLSPTAEEVITS
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHH
LVRNEVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDAYSFDINKEGLQKSYDA
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MHDAYCKAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSTESDYAANIEKA
HHHHHHHHHHHCCCEEECEEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHH
EAVAPTTERAAPTQEMTTVDTPNAKTIAELVEQHGIAIEKTVKTLFVKASDEVDADIIAL
HHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCEEEEE
IIRGDHELNEVKAENLPQVASPLEMADEAQLRDLIGAGAGSLGPVGLELPFIVDRSVAVM
EEECCCHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEECCEEECCCHHHH
SDFGTGANIDGKHFFGVNWDRDVQLGQVEDLRNVVEGDPSPCGKGTLQLKRGIEVGHIFQ
HCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHH
LGTAYSEAMNCGVLDANGKNVILEMGCYGIGVSRVVASAIEQNNDEYGIVWPEAIAPFTV
HHHHHHHHCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCHHHCCEEE
AIVPMNMHKSERVKEAAEKLYAELTAMGIEVLFDDRKERPGVMFKDIELIGIPHTIVIGD
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEECEEEEECCEEEEECC
RSMDEGHFEYKNRRANTKEAVEMANIVEHIKAQLS
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDENMRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRRLASGLYTWLPTGLRVLRKVENI
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRQEIDNAGAIETLMPVVQPFELWEETGRSEKMGPELLRFTDRHVRPFVLSPTAEEVITS
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHH
LVRNEVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDAYSFDINKEGLQKSYDA
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MHDAYCKAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSTESDYAANIEKA
HHHHHHHHHHHCCCEEECEEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHH
EAVAPTTERAAPTQEMTTVDTPNAKTIAELVEQHGIAIEKTVKTLFVKASDEVDADIIAL
HHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCEEEEE
IIRGDHELNEVKAENLPQVASPLEMADEAQLRDLIGAGAGSLGPVGLELPFIVDRSVAVM
EEECCCHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEECCEEECCCHHHH
SDFGTGANIDGKHFFGVNWDRDVQLGQVEDLRNVVEGDPSPCGKGTLQLKRGIEVGHIFQ
HCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHH
LGTAYSEAMNCGVLDANGKNVILEMGCYGIGVSRVVASAIEQNNDEYGIVWPEAIAPFTV
HHHHHHHHCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCHHHCCEEE
AIVPMNMHKSERVKEAAEKLYAELTAMGIEVLFDDRKERPGVMFKDIELIGIPHTIVIGD
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEECEEEEECCEEEEECC
RSMDEGHFEYKNRRANTKEAVEMANIVEHIKAQLS
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA