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The map label for this gene is trmE [H]

Identifier: 55980900

GI number: 55980900

Start: 881129

End: 882427

Strand: Reverse

Name: trmE [H]

Synonym: TTHA0931

Alternate gene names: 55980900

Gene position: 882427-881129 (Counterclockwise)

Preceding gene: 55980901

Following gene: 55980896

Centisome position: 47.71

GC content: 70.67

Gene sequence:

>1299_bases
GTGAACCTTAAAGACCCCATCTGCGCCATCGCCACCCCCCCCGGGAAGGGGGCCATCGGGGTGGTGCGGCTTTCCGGCGA
GGGCGCCCTGGAGATCGCCGCAAGGGTGTGGCGGGGTAAGGACCCGAGGCGCCTTAAGGGCGGGCGCTTCGCCCTGGGCG
AGGTGGTGGACCCTAAGACGGGGGAGGCCATTGACCAGGCGATCCTCCTCGTCTTCCGCGCCCCCCGTTCCTACACGGGG
GAGGACCTGGTGGAGTTCCAGACCCACGGCTCTCCCGCGGTGCTCCGCCGGGTGATGGAGGTCCTGGTGGCGGAAGGGGC
AAGGCCCGCGGGGCGCGGGGAGTTTACCTTTCGCGCTTACCTGAACGGCAAGATGGACCTGGCCCAGGCGGAGGCGGTCT
TGGCCCTCATTGAGGCGGAAGGGGAGCTTGCCCGGAGGCAGGCCCTGAGGGCGCTGGAGGGGGCCCTTTCCCGCCGGATT
GAGGCCTTGGAGAACCGGCTTCTAAACCTCCTCGCCCACATCCAGGCCCTCCTGGACTACCCCGAGGAGGGGGTGGAGCC
CCTGGAGGCGGAAAGGACCATCCGGGAGGTCCTCGCCGAGGTGGAGGCCCTCCTCGCCCAGGCCAAGGCCTCCCGCCTGG
CCCAGAAGGGGGCCAGGCTCGCCCTCATCGGGGCCCCCAACGCCGGGAAGAGCAGCCTCCTCAACGCCCTTTTGGGGTAC
GAGCGGGCCCTCGTCTCCCCCATCCCCGGAACCACCCGGGACTACCTCGAGGCCCCCCTGGAGCTTTTCGGCATCCCCCT
CGTGGCCGTGGACACCGCCGGGGTGCGGGAGACGGAGGACCCCGTGGAGCGGATGGGGGTGGAGAGGGCCTTAGGAATCG
CCGAGGAAGCCGACCTGGTCCTTTACGTGGTGGACCGCTCCCAGCCCAAGCCCGCCCCGCCCCCCCTGCCTTGGGCGAGG
ACGCTGAAGGTGGCCACCAAGAGCGACCTTCCCCCGGCCTGGGAGGACCCGGAGTTCCTCCCCGTCTCCAGCCTCACGGG
AGAGGGTCTGGACCGGCTCAAGGAGGCGGTAAGGGAGGCCCTTTTGGGGAGGGAGGGGGGGGAGGTCCTCCTCACCGAGC
GCCAGGTGGAAGCCCTCCTCCGGGCTAGGGAGCGCCTGGAGGAGGCCCTCTCCCTCCCCGAGGACCTCATGGGCCTCGCC
CTGGAGGAGGCGGCCCGGGCCCTCGCCCTCCTCACGGGCAAGGAGGTGGCGGAGGAGGTGGTGGCCCGGGTCTTCCAGAA
CTTCTGCGTGGGGAAGTAA

Upstream 100 bases:

>100_bases
GAAAGGTGGTGCTGCCCCTGCACTCCGGCGAGATACCTTGGGGTCTTTTTAAGACCGTTCTGAAACAAGCGGGGATCACC
GAAGAGGAGTTTCGCCGGGC

Downstream 100 bases:

>100_bases
ACCCCGTGCCGGTCTGCGCCGGCATGGGGCCCCGGCAAAACAACCGCCCGCTCCTGGCTCAGTGGCGGAAGTGGCGCACG
CCGGTGAAGACCATGGCGAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNLKDPICAIATPPGKGAIGVVRLSGEGALEIAARVWRGKDPRRLKGGRFALGEVVDPKTGEAIDQAILLVFRAPRSYTG
EDLVEFQTHGSPAVLRRVMEVLVAEGARPAGRGEFTFRAYLNGKMDLAQAEAVLALIEAEGELARRQALRALEGALSRRI
EALENRLLNLLAHIQALLDYPEEGVEPLEAERTIREVLAEVEALLAQAKASRLAQKGARLALIGAPNAGKSSLLNALLGY
ERALVSPIPGTTRDYLEAPLELFGIPLVAVDTAGVRETEDPVERMGVERALGIAEEADLVLYVVDRSQPKPAPPPLPWAR
TLKVATKSDLPPAWEDPEFLPVSSLTGEGLDRLKEAVREALLGREGGEVLLTERQVEALLRARERLEEALSLPEDLMGLA
LEEAARALALLTGKEVAEEVVARVFQNFCVGK

Sequences:

>Translated_432_residues
MNLKDPICAIATPPGKGAIGVVRLSGEGALEIAARVWRGKDPRRLKGGRFALGEVVDPKTGEAIDQAILLVFRAPRSYTG
EDLVEFQTHGSPAVLRRVMEVLVAEGARPAGRGEFTFRAYLNGKMDLAQAEAVLALIEAEGELARRQALRALEGALSRRI
EALENRLLNLLAHIQALLDYPEEGVEPLEAERTIREVLAEVEALLAQAKASRLAQKGARLALIGAPNAGKSSLLNALLGY
ERALVSPIPGTTRDYLEAPLELFGIPLVAVDTAGVRETEDPVERMGVERALGIAEEADLVLYVVDRSQPKPAPPPLPWAR
TLKVATKSDLPPAWEDPEFLPVSSLTGEGLDRLKEAVREALLGREGGEVLLTERQVEALLRARERLEEALSLPEDLMGLA
LEEAARALALLTGKEVAEEVVARVFQNFCVGK
>Mature_432_residues
MNLKDPICAIATPPGKGAIGVVRLSGEGALEIAARVWRGKDPRRLKGGRFALGEVVDPKTGEAIDQAILLVFRAPRSYTG
EDLVEFQTHGSPAVLRRVMEVLVAEGARPAGRGEFTFRAYLNGKMDLAQAEAVLALIEAEGELARRQALRALEGALSRRI
EALENRLLNLLAHIQALLDYPEEGVEPLEAERTIREVLAEVEALLAQAKASRLAQKGARLALIGAPNAGKSSLLNALLGY
ERALVSPIPGTTRDYLEAPLELFGIPLVAVDTAGVRETEDPVERMGVERALGIAEEADLVLYVVDRSQPKPAPPPLPWAR
TLKVATKSDLPPAWEDPEFLPVSSLTGEGLDRLKEAVREALLGREGGEVLLTERQVEALLRARERLEEALSLPEDLMGLA
LEEAARALALLTGKEVAEEVVARVFQNFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=463, Percent_Identity=38.8768898488121, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI193082993, Length=447, Percent_Identity=39.1498881431767, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI193082988, Length=494, Percent_Identity=36.4372469635627, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI2367268, Length=460, Percent_Identity=37.1739130434783, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI87082120, Length=90, Percent_Identity=45.5555555555556, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=449, Percent_Identity=31.1804008908686, Blast_Score=174, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6323665, Length=496, Percent_Identity=28.8306451612903, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45550813, Length=477, Percent_Identity=31.2368972746331, Blast_Score=180, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 46692; Mature: 46692

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKDPICAIATPPGKGAIGVVRLSGEGALEIAARVWRGKDPRRLKGGRFALGEVVDPKT
CCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCC
GEAIDQAILLVFRAPRSYTGEDLVEFQTHGSPAVLRRVMEVLVAEGARPAGRGEFTFRAY
CHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
LNGKMDLAQAEAVLALIEAEGELARRQALRALEGALSRRIEALENRLLNLLAHIQALLDY
ECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PEEGVEPLEAERTIREVLAEVEALLAQAKASRLAQKGARLALIGAPNAGKSSLLNALLGY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
ERALVSPIPGTTRDYLEAPLELFGIPLVAVDTAGVRETEDPVERMGVERALGIAEEADLV
HHHHCCCCCCCCHHHHHCCHHHHCCCEEEECCCCCCCCCCHHHHHCHHHHHCCCCCCCEE
LYVVDRSQPKPAPPPLPWARTLKVATKSDLPPAWEDPEFLPVSSLTGEGLDRLKEAVREA
EEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHH
LLGREGGEVLLTERQVEALLRARERLEEALSLPEDLMGLALEEAARALALLTGKEVAEEV
HHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARVFQNFCVGK
HHHHHHHHCCCC
>Mature Secondary Structure
MNLKDPICAIATPPGKGAIGVVRLSGEGALEIAARVWRGKDPRRLKGGRFALGEVVDPKT
CCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCC
GEAIDQAILLVFRAPRSYTGEDLVEFQTHGSPAVLRRVMEVLVAEGARPAGRGEFTFRAY
CHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
LNGKMDLAQAEAVLALIEAEGELARRQALRALEGALSRRIEALENRLLNLLAHIQALLDY
ECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PEEGVEPLEAERTIREVLAEVEALLAQAKASRLAQKGARLALIGAPNAGKSSLLNALLGY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
ERALVSPIPGTTRDYLEAPLELFGIPLVAVDTAGVRETEDPVERMGVERALGIAEEADLV
HHHHCCCCCCCCHHHHHCCHHHHCCCEEEECCCCCCCCCCHHHHHCHHHHHCCCCCCCEE
LYVVDRSQPKPAPPPLPWARTLKVATKSDLPPAWEDPEFLPVSSLTGEGLDRLKEAVREA
EEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHH
LLGREGGEVLLTERQVEALLRARERLEEALSLPEDLMGLALEEAARALALLTGKEVAEEV
HHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARVFQNFCVGK
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA