Click here to switch to the map view.

The map label for this gene is holA [C]

Identifier: 55980757

GI number: 55980757

Start: 754477

End: 755355

Strand: Reverse

Name: holA [C]

Synonym: TTHA0788

Alternate gene names: 55980757

Gene position: 755355-754477 (Counterclockwise)

Preceding gene: 55980758

Following gene: 55980756

Centisome position: 40.84

GC content: 71.33

Gene sequence:

>879_bases
ATGGTCATCGCCTTCACCGGGGATCCCTTCCTGGCGCGGGAGGCCCTCTTAGAGGAGGCCAGGCTTAGGGGCCTTTCCCG
CTTCACCGAGCCCACCCCGGAGGCCCTGGCCCAGGCCCTGGCCCCAGGGCTTTTCGGGGGCGGGGGGGCGATGCTGGACC
TGAGGGAGGTGGGGGAGGCGGAGTGGAAGGCCCTAAAGCCCCTTTTGGAAAGCGTGCCCGAGGGCGTCCCCGTCCTCCTC
CTGGACCCTAAGCCAAGCCCCTCCCGGGCGGCCTTCTACCGGAACCGGGAAAGGCGGGACTTCCCCACCCCCAAGGGGAA
GGACCTGGTGCGGCACCTGGAAAACCGGGCCAAGCGCCTGGGGCTCAGGCTCCCGGGCGGGGTGGCCCAGTACCTGGCCT
CCCTGGAGGGGGACCTCGAGGCCCTGGAGCGGGAGCTGGAGAAGCTTGCCCTCCTCTCCCCACCCCTCACCCTGGAGAAG
GTGGAGAAGGTGGTGGCCCTGAGGCCCCCCCTCACGGGCTTTGACCTGGTGCGCTCCGTCCTGGAGAAGGACCCCAAGGA
GGCCCTCCTGCGCCTCAGGCGCCTCAAGGAGGAGGGGGAGGAGCCCCTCAGGCTCCTCGGGGCCCTCTCCTGGCAGTTCG
CCCTCCTCGCCCGGGCCTTCTTCCTCCTCCGGGAAAACCCCAGGCCCAAGGAGGAGGACCTCGCCCGCCTCGAGGCCCAC
CCCTACGCCGCCCGCCGCGCCCTGGAGGCGGCGAAGCGCCTCACGGAAGAGGCCCTCAAGGAGGCCCTGGACGCCCTCAT
GGAGGCGGAAAAGAGGGCCAAGGGGGGGAAAGACCCGTGGCTCGCCCTGGAGGCGGCGGTCCTCCGCCTCGCCGGTTGA

Upstream 100 bases:

>100_bases
GAGGGCACCCACGACGTCCACACCCTGGTCCTGGGACGGGAGATCACCGGCCTGAACGCCTTCTAAAGGCCCGCCGGGGC
CTTTGGCGGACACTTAAGCC

Downstream 100 bases:

>100_bases
CCCGCGGGTCAGTCCCGGGTAGACTCGGGGCATGCTCAAGCCCGAGCTCGTGGAGGCGGTGGGGGACGACCTGGACTGGC
TCCTCTTAAACGCGGCCTAC

Product: DNA polymerase III subunit delta

Products: Pyrophosphate; DNA [C]

Alternate protein names: DNA Polymerase III Subunit Delta

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MVIAFTGDPFLAREALLEEARLRGLSRFTEPTPEALAQALAPGLFGGGGAMLDLREVGEAEWKALKPLLESVPEGVPVLL
LDPKPSPSRAAFYRNRERRDFPTPKGKDLVRHLENRAKRLGLRLPGGVAQYLASLEGDLEALERELEKLALLSPPLTLEK
VEKVVALRPPLTGFDLVRSVLEKDPKEALLRLRRLKEEGEEPLRLLGALSWQFALLARAFFLLRENPRPKEEDLARLEAH
PYAARRALEAAKRLTEEALKEALDALMEAEKRAKGGKDPWLALEAAVLRLAG

Sequences:

>Translated_292_residues
MVIAFTGDPFLAREALLEEARLRGLSRFTEPTPEALAQALAPGLFGGGGAMLDLREVGEAEWKALKPLLESVPEGVPVLL
LDPKPSPSRAAFYRNRERRDFPTPKGKDLVRHLENRAKRLGLRLPGGVAQYLASLEGDLEALERELEKLALLSPPLTLEK
VEKVVALRPPLTGFDLVRSVLEKDPKEALLRLRRLKEEGEEPLRLLGALSWQFALLARAFFLLRENPRPKEEDLARLEAH
PYAARRALEAAKRLTEEALKEALDALMEAEKRAKGGKDPWLALEAAVLRLAG
>Mature_292_residues
MVIAFTGDPFLAREALLEEARLRGLSRFTEPTPEALAQALAPGLFGGGGAMLDLREVGEAEWKALKPLLESVPEGVPVLL
LDPKPSPSRAAFYRNRERRDFPTPKGKDLVRHLENRAKRLGLRLPGGVAQYLASLEGDLEALERELEKLALLSPPLTLEK
VEKVVALRPPLTGFDLVRSVLEKDPKEALLRLRRLKEEGEEPLRLLGALSWQFALLARAFFLLRENPRPKEEDLARLEAH
PYAARRALEAAKRLTEEALKEALDALMEAEKRAKGGKDPWLALEAAVLRLAG

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Delta Subunit Seems To Interact With The Gamma Subunit To Transfer The B

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=207, Percent_Identity=32.8502415458937, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 32427; Mature: 32427

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIAFTGDPFLAREALLEEARLRGLSRFTEPTPEALAQALAPGLFGGGGAMLDLREVGEA
CEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCHH
EWKALKPLLESVPEGVPVLLLDPKPSPSRAAFYRNRERRDFPTPKGKDLVRHLENRAKRL
HHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
GLRLPGGVAQYLASLEGDLEALERELEKLALLSPPLTLEKVEKVVALRPPLTGFDLVRSV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
LEKDPKEALLRLRRLKEEGEEPLRLLGALSWQFALLARAFFLLRENPRPKEEDLARLEAH
HHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
PYAARRALEAAKRLTEEALKEALDALMEAEKRAKGGKDPWLALEAAVLRLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVIAFTGDPFLAREALLEEARLRGLSRFTEPTPEALAQALAPGLFGGGGAMLDLREVGEA
CEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCHH
EWKALKPLLESVPEGVPVLLLDPKPSPSRAAFYRNRERRDFPTPKGKDLVRHLENRAKRL
HHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
GLRLPGGVAQYLASLEGDLEALERELEKLALLSPPLTLEKVEKVVALRPPLTGFDLVRSV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
LEKDPKEALLRLRRLKEEGEEPLRLLGALSWQFALLARAFFLLRENPRPKEEDLARLEAH
HHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
PYAARRALEAAKRLTEEALKEALDALMEAEKRAKGGKDPWLALEAAVLRLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: Deoxynucleoside Triphosphate [C]

Specific reaction: (n)Deoxynucleoside Triphosphate = (n)Pyrophosphate + DNA(n) [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10 -Phenanthroline; 2', 3'-Dideoxythymidine 5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA