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The map label for this gene is proP [H]

Identifier: 54295231

GI number: 54295231

Start: 2637297

End: 2638529

Strand: Reverse

Name: proP [H]

Synonym: lpl2314

Alternate gene names: 54295231

Gene position: 2638529-2637297 (Counterclockwise)

Preceding gene: 54295232

Following gene: 54295229

Centisome position: 78.86

GC content: 38.2

Gene sequence:

>1233_bases
ATGGATAAAAAAATTATTGCGGCAGCCTCTTTTGGCACCTTGTTTGAAATGTACGACTATGCCATTTATGGGTTTATGGC
GCCCATTTTGGCGCCTCTTTTTTTTCCTGCCACTGATAAACACACTGCTCTCATCGCAACATTTGGTATTTTTGCCGCAG
GTTTTCTTGTTCGGCCATTCTCTGCTTATTGGTTAGGGAATCTGGGTGACAAAATTGGCCGCAAAAAAACATTGACTATG
ACTCTTATGGTCATGACTTTTTCATCAAGTTTTATTGGTTTATTACCTGCTTATCAAGAGGCGGGCATTCTCGCTCCGAT
ATTACTCACGCTATGTCGATTAACTCAAGGGGTTTCTGTAGCAGGGGAAATGACACTAGCCAGTATTTACATTTATGAAA
ATGTGAACATGCGTCATAAAGGTTTAGCAGTCAGTTTCGTCACTTTAGGTTCTATTTGTGGATTGTTGCTTGCAATAATA
GTAAGCAAAATAACCCTGATATTTTTTTCCAGTCAGTCTCCCGATTTTCTCTGGCGCTTCCCATTTTTAATAGGAATATT
CGTAGGTTTTATCGGACTTTATTTGCGAAGTCAAACCAGAGAATTAACCGATTTTGCAAAAGTAATTGTTCAAAGACAAT
CTCATCCCATACTGAATGCCTGGCGTTATCGGAAAAAAGCAATTGCTGCTATTTTTCTTTTGGCAATTTATTGCAACAGT
AGCTTTTATACATTTTTTGTTTATATGCCTTCATATCTGCAACAGCAGGAGTTGCCATTAACTACAGCGATGGATGTCAT
TATCATAGGGATGATTTGTTATAGCATGGGTCTCTTATTATCAGGTTTCACCTCTGATATTTTAGGCAGGCGCTTTCCTT
TGGCAGTCAGTTTAATTGCAACTTTATGTACCGTTTTGGTAATAAATTATTTGATCAATACAGGTACTTTATTACACATT
ACTGTAGCCTGCAGTGTTTTGGCTGTCATTATCTCTTTTTTTATTGGAAGCGCATTTACCGCCAGCCTTGAACAATTACC
GCAGGAAGAGCGCGCCAGTGGCTTTACTTTATTTTTTAACTTGTCTAATTCCCTATTTGGAGGCACAGCACCACTCATTA
TTTTGCTATTAAGTTATGCTTTCATCCATTTTGCATTTTCTATTTATTTGATGGTGATGGCTTTGTTGACTTTTCCTGCC
CTGTGGTTGATGCAGGATAAAGATAGCACTTAA

Upstream 100 bases:

>100_bases
CCAGTCAAGGATGAAGTGGATTCAGAGAATCAAGCTCAGAAATCAATAGAGTTAAAGTAAGCTGTATTACTACCTGTGAA
ACAAAAGCAACAGCTTGAAC

Downstream 100 bases:

>100_bases
TTTATAGAATCTTTAGCCTTGATGTAGTGAAGCCTATTCAAGGCTAAGCAGGATGGCTTATTTTTCTATGATTATCTGTG
ACATGTCAACTTGCAAAAGA

Product: hypothetical protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTALIATFGIFAAGFLVRPFSAYWLGNLGDKIGRKKTLTM
TLMVMTFSSSFIGLLPAYQEAGILAPILLTLCRLTQGVSVAGEMTLASIYIYENVNMRHKGLAVSFVTLGSICGLLLAII
VSKITLIFFSSQSPDFLWRFPFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPILNAWRYRKKAIAAIFLLAIYCNS
SFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLLHI
TVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTFPA
LWLMQDKDST

Sequences:

>Translated_410_residues
MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTALIATFGIFAAGFLVRPFSAYWLGNLGDKIGRKKTLTM
TLMVMTFSSSFIGLLPAYQEAGILAPILLTLCRLTQGVSVAGEMTLASIYIYENVNMRHKGLAVSFVTLGSICGLLLAII
VSKITLIFFSSQSPDFLWRFPFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPILNAWRYRKKAIAAIFLLAIYCNS
SFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLLHI
TVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTFPA
LWLMQDKDST
>Mature_410_residues
MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTALIATFGIFAAGFLVRPFSAYWLGNLGDKIGRKKTLTM
TLMVMTFSSSFIGLLPAYQEAGILAPILLTLCRLTQGVSVAGEMTLASIYIYENVNMRHKGLAVSFVTLGSICGLLLAII
VSKITLIFFSSQSPDFLWRFPFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPILNAWRYRKKAIAAIFLLAIYCNS
SFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLLHI
TVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTFPA
LWLMQDKDST

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=410, Percent_Identity=33.4146341463415, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1788942, Length=400, Percent_Identity=32.5, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789941, Length=416, Percent_Identity=28.6057692307692, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1788292, Length=427, Percent_Identity=25.7611241217799, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082404, Length=314, Percent_Identity=22.6114649681529, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI87082231, Length=464, Percent_Identity=22.6293103448276, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45473; Mature: 45473

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTALIATFGIFAAGFLVRPF
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
SAYWLGNLGDKIGRKKTLTMTLMVMTFSSSFIGLLPAYQEAGILAPILLTLCRLTQGVSV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCH
AGEMTLASIYIYENVNMRHKGLAVSFVTLGSICGLLLAIIVSKITLIFFSSQSPDFLWRF
HCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHH
PFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPILNAWRYRKKAIAAIFLLAIYCNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSLIA
CCEEHEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TLCTVLVINYLINTGTLLHITVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEE
LSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTFPALWLMQDKDST
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTALIATFGIFAAGFLVRPF
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
SAYWLGNLGDKIGRKKTLTMTLMVMTFSSSFIGLLPAYQEAGILAPILLTLCRLTQGVSV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCH
AGEMTLASIYIYENVNMRHKGLAVSFVTLGSICGLLLAIIVSKITLIFFSSQSPDFLWRF
HCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHH
PFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPILNAWRYRKKAIAAIFLLAIYCNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSLIA
CCEEHEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TLCTVLVINYLINTGTLLHITVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEE
LSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTFPALWLMQDKDST
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]