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The map label for this gene is radA [H]

Identifier: 54295166

GI number: 54295166

Start: 2565641

End: 2566993

Strand: Reverse

Name: radA [H]

Synonym: lpl2246

Alternate gene names: 54295166

Gene position: 2566993-2565641 (Counterclockwise)

Preceding gene: 54295171

Following gene: 54295165

Centisome position: 76.73

GC content: 46.56

Gene sequence:

>1353_bases
ATGAAAGTGAAAACCCAGTTTGTTTGTAATCAATGCGCGGCCATTTTTAAACAATGGGCAGGACAATGTACTCAATGTGG
TGCCTGGAATTCCATTGTTGAAGAAGGAATTATGCCTGAAAGCAAGACTTTACGAAGCGGGCAATGGGCTAATCAGCGCT
CTGTTATCACTGCTGTTGAAGACGTCGTCATGGACAAAATAGTACGTATGGATTGTGGTATATCCGAGCTCAACCGGGTT
TTGGGAGGGGGGCTGGTTTATGGCTCGGTTGTTTTGATTGGAGGGGATCCGGGGATCGGTAAATCCACCTTACTGTTGCA
AACGCTGGCTAATTTATCTATGCAAGAAACAGTATTGTATGTGACGGGGGAAGAGTCATTACAACAAGTTGCCATGCGTG
CCCAAAGATTGGGACTACCTTTGGCCGGACTGCGGCTATTGGCAGAAACTCAGGTTGAATCCATCATTGCTCAAGCCCAA
AAGGAAAAGCCGCGAATTATTGTCGTCGATTCAGTACAAACGATATTCACTGAAGCCATAAGTTCTGCTCCGGGAGGAGT
TAGTCAGGTCCGGGAATCAGCAGCGCAATTGGTGCGTTATGCCAAGATGACTAATACGGCGATTTTCCTGGTAGGGCATG
TCACTAAGGAAGGAACCTTGGCCGGGCCTCGAGTGTTAGAACATATGGTTGATAGTGTTCTTTATTTTGAAGGGCAAAAC
GACAGTCGTTTCAGAGTTATTCGCGCCATTAAAAATCGTTTTGGCGCTGTCAATGAATTAGGTGTATTTGCCATGACAGA
CAAAGGTCTGAAGGAAGTGGCCAATCCCTCAGCTATTTTTTTATCTCGCCAGCCTGATCCTACGCCAGGCAGCGCAGTGA
TGGTAACTTGGGAAGGATCAAGACCCATGTTGGTAGAAGTACAGGCATTGGTTGATGAGGCCCACGGCCAGCAAGCGAAA
CGCGTTACTGCCGGGCTTGAGCATAATCGCCTGGCGATTTTGTTAGCTGTCTTGCACCGCCATGGAGGTATTGCCACTTA
CGATCAGGACGTTTTTATCAATGTAGTAGGGGGAGTTAAGGTAACGGAAACAGCGAGTGATTTAGCATTGTTGGCCGCGG
TGATTTCCAGTTTGCGTAATCGTATATTTGACAATGAAACCATCATATTCGGTGAGGTAGGATTGGCGGGAGAAATCAGA
CCGGTGCAAAGTGGTCAGGAACGCATTAAGGAAGCTATAAAACATGGATTCAAGCGTGCTATAGTTCCTTTTGCCAATGC
TCCCAAACAGCAAATCGATTCCATGACGGTAGAGCCAGTTAAACATTTGCGAGATGTATTGGAGAAAATGTGA

Upstream 100 bases:

>100_bases
AGATAAGTTCTGGTTCATTTAAAAATTATTTAAGGCAAATATGTTCAGCGCGAGTTATCATGAATGTACTAAATAAAATG
CTTGAATCAAGGCAAAAATT

Downstream 100 bases:

>100_bases
GTTATCAAAAATGAATCACACCGGAATATAAAATGAATGAAGATACTATCCGTGATTTTAATCGTAATGGTGCCGTATGT
CTTCGCCAATTTTTAAATGC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKVKTQFVCNQCAAIFKQWAGQCTQCGAWNSIVEEGIMPESKTLRSGQWANQRSVITAVEDVVMDKIVRMDCGISELNRV
LGGGLVYGSVVLIGGDPGIGKSTLLLQTLANLSMQETVLYVTGEESLQQVAMRAQRLGLPLAGLRLLAETQVESIIAQAQ
KEKPRIIVVDSVQTIFTEAISSAPGGVSQVRESAAQLVRYAKMTNTAIFLVGHVTKEGTLAGPRVLEHMVDSVLYFEGQN
DSRFRVIRAIKNRFGAVNELGVFAMTDKGLKEVANPSAIFLSRQPDPTPGSAVMVTWEGSRPMLVEVQALVDEAHGQQAK
RVTAGLEHNRLAILLAVLHRHGGIATYDQDVFINVVGGVKVTETASDLALLAAVISSLRNRIFDNETIIFGEVGLAGEIR
PVQSGQERIKEAIKHGFKRAIVPFANAPKQQIDSMTVEPVKHLRDVLEKM

Sequences:

>Translated_450_residues
MKVKTQFVCNQCAAIFKQWAGQCTQCGAWNSIVEEGIMPESKTLRSGQWANQRSVITAVEDVVMDKIVRMDCGISELNRV
LGGGLVYGSVVLIGGDPGIGKSTLLLQTLANLSMQETVLYVTGEESLQQVAMRAQRLGLPLAGLRLLAETQVESIIAQAQ
KEKPRIIVVDSVQTIFTEAISSAPGGVSQVRESAAQLVRYAKMTNTAIFLVGHVTKEGTLAGPRVLEHMVDSVLYFEGQN
DSRFRVIRAIKNRFGAVNELGVFAMTDKGLKEVANPSAIFLSRQPDPTPGSAVMVTWEGSRPMLVEVQALVDEAHGQQAK
RVTAGLEHNRLAILLAVLHRHGGIATYDQDVFINVVGGVKVTETASDLALLAAVISSLRNRIFDNETIIFGEVGLAGEIR
PVQSGQERIKEAIKHGFKRAIVPFANAPKQQIDSMTVEPVKHLRDVLEKM
>Mature_450_residues
MKVKTQFVCNQCAAIFKQWAGQCTQCGAWNSIVEEGIMPESKTLRSGQWANQRSVITAVEDVVMDKIVRMDCGISELNRV
LGGGLVYGSVVLIGGDPGIGKSTLLLQTLANLSMQETVLYVTGEESLQQVAMRAQRLGLPLAGLRLLAETQVESIIAQAQ
KEKPRIIVVDSVQTIFTEAISSAPGGVSQVRESAAQLVRYAKMTNTAIFLVGHVTKEGTLAGPRVLEHMVDSVLYFEGQN
DSRFRVIRAIKNRFGAVNELGVFAMTDKGLKEVANPSAIFLSRQPDPTPGSAVMVTWEGSRPMLVEVQALVDEAHGQQAK
RVTAGLEHNRLAILLAVLHRHGGIATYDQDVFINVVGGVKVTETASDLALLAAVISSLRNRIFDNETIIFGEVGLAGEIR
PVQSGQERIKEAIKHGFKRAIVPFANAPKQQIDSMTVEPVKHLRDVLEKM

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=449, Percent_Identity=57.6837416481069, Blast_Score=531, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48903; Mature: 48903

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKTQFVCNQCAAIFKQWAGQCTQCGAWNSIVEEGIMPESKTLRSGQWANQRSVITAVE
CCCCHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHH
DVVMDKIVRMDCGISELNRVLGGGLVYGSVVLIGGDPGIGKSTLLLQTLANLSMQETVLY
HHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCHHHEEEE
VTGEESLQQVAMRAQRLGLPLAGLRLLAETQVESIIAQAQKEKPRIIVVDSVQTIFTEAI
EECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
SSAPGGVSQVRESAAQLVRYAKMTNTAIFLVGHVTKEGTLAGPRVLEHMVDSVLYFEGQN
HCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHEEEECCC
DSRFRVIRAIKNRFGAVNELGVFAMTDKGLKEVANPSAIFLSRQPDPTPGSAVMVTWEGS
CCHHHHHHHHHHHHCCHHHCCEEEECCHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCC
RPMLVEVQALVDEAHGQQAKRVTAGLEHNRLAILLAVLHRHGGIATYDQDVFINVVGGVK
CCEEEHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEECCCEEEEEECCEE
VTETASDLALLAAVISSLRNRIFDNETIIFGEVGLAGEIRPVQSGQERIKEAIKHGFKRA
EECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IVPFANAPKQQIDSMTVEPVKHLRDVLEKM
HCCCCCCCHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVKTQFVCNQCAAIFKQWAGQCTQCGAWNSIVEEGIMPESKTLRSGQWANQRSVITAVE
CCCCHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHH
DVVMDKIVRMDCGISELNRVLGGGLVYGSVVLIGGDPGIGKSTLLLQTLANLSMQETVLY
HHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCHHHEEEE
VTGEESLQQVAMRAQRLGLPLAGLRLLAETQVESIIAQAQKEKPRIIVVDSVQTIFTEAI
EECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
SSAPGGVSQVRESAAQLVRYAKMTNTAIFLVGHVTKEGTLAGPRVLEHMVDSVLYFEGQN
HCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHEEEECCC
DSRFRVIRAIKNRFGAVNELGVFAMTDKGLKEVANPSAIFLSRQPDPTPGSAVMVTWEGS
CCHHHHHHHHHHHHCCHHHCCEEEECCHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCC
RPMLVEVQALVDEAHGQQAKRVTAGLEHNRLAILLAVLHRHGGIATYDQDVFINVVGGVK
CCEEEHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEECCCEEEEEECCEE
VTETASDLALLAAVISSLRNRIFDNETIIFGEVGLAGEIRPVQSGQERIKEAIKHGFKRA
EECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IVPFANAPKQQIDSMTVEPVKHLRDVLEKM
HCCCCCCCHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]