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The map label for this gene is pilT [H]

Identifier: 54295163

GI number: 54295163

Start: 2561381

End: 2562502

Strand: Reverse

Name: pilT [H]

Synonym: lpl2243

Alternate gene names: 54295163

Gene position: 2562502-2561381 (Counterclockwise)

Preceding gene: 54295164

Following gene: 54295162

Centisome position: 76.59

GC content: 37.25

Gene sequence:

>1122_bases
ATGGATATTACTCCTTTTTTTAAGCTCATGGTCGATAAAGGTGCCTCAGATTTGTTTTTCAGTGTCGGGGCACCACCAAA
TATTAAAATCGAGGGCATCACATCACCTATTGGCCAGGTTCCTATCAAATCGAATCAAATGGCAGAAATTGTTTTATCTA
TTATGAATGACGCACAGCAAAAAGAATTTGAGTCTAAAATGGAACTCAATATGGCTTTTTCATTAGCTGGAGTAGGGCGT
TTCCGAATCAATTTATTCAGGCAACGTGGCGAAGTGGCTATGGTGGTGCGTTACATCAAGGGCGAAATACCTTCTATAGA
AGATTTAAATCTTCCTCAAATTTTAAAGTCTCTGGTCTTGGAGCTGCGAGGTTTAGTATTGGTTGTTGGGTCCACGGGAT
CGGGTAAATCAACGACTCTGGCCGCGATGATCAACCACCGAAATGAAAATCACAGAGGGCATATTTTAACAATAGAGGAT
CCTATTGAATATATACATCAACATAAAAAATCAATAGTTGATCAACGAGAAATAGGTATTGATACTCTAAGCTATGACAA
TGCATTGAAAAACGCAATGAGAGAAGCGCCTGATGTGATATTAATTGGTGAAATTCGTGATCTTAACTCAATGAAACATG
CGATAGCTTATGCTGAAACGGGACATTTGTGCATTTCTACTTTGCATGCCAATAACGCCAATCAGGCTCTGGATAGAGTG
ATTAATTTTTTCCCCGATGAGGCAAGACAACAGATATTATTAGATTTATCTCTTAATTTAAAAGCAATCATTTCATTACG
TTTGATACCAGGAGTTGACAATCAACGTGTCGCAGCGGTGGAGATCTTGATTAACTCTCCTTACATTTCCGATTTGATTG
AAAAAGGGAAGATAGACGATATTAAAGAAGCCATGGCAAGAAGCACGGAACAAGGCATGCAGACTTTCGATCAGGCTTTA
TTTAACTTATATAAAGAAGGAAAAATTACCAAAGAAAATGCTATTAAGTATGCTGATTCAAAAAACAATGTGGGTTTACA
AATTCGATTGGGCGAAGAAAAAAGCTTTAATATTCAAGATGAGAATTTACAAATACAACCCGATGATATTGATAAGGTTT
GA

Upstream 100 bases:

>100_bases
TATTGAGTTAAGCAATCAGGAAAATAAGTACAAGACAGAGCCTAGCAAATTCAGGAGTCTCTACTAAACTTAAAAATATA
ATAATATTTTGAGTTAACCT

Downstream 100 bases:

>100_bases
TTCGTTGTGAATTAATTTTGAACATTGTTTTGAATGAAACATTGTGATAAAATTATAGTCTAATTTGTTAATTTTTCTTC
CATTCCATGCCTAGAGTTTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKSNQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGR
FRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDDIKEAMARSTEQGMQTFDQAL
FNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKSFNIQDENLQIQPDDIDKV

Sequences:

>Translated_373_residues
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKSNQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGR
FRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDDIKEAMARSTEQGMQTFDQAL
FNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKSFNIQDENLQIQPDDIDKV
>Mature_373_residues
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKSNQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGR
FRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDDIKEAMARSTEQGMQTFDQAL
FNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKSFNIQDENLQIQPDDIDKV

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=331, Percent_Identity=35.6495468277946, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1789723, Length=158, Percent_Identity=39.873417721519, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786296, Length=263, Percent_Identity=29.277566539924, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41675; Mature: 41675

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKSNQMAEIVLSIMNDAQQ
CCCCHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
KEFESKMELNMAFSLAGVGRFRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVL
HHHHHHHHHHHEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIEDPIEYIHQHKKSIVDQREIGI
HHCCEEEEEECCCCCCCHHHEEHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCC
DTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
CEEHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDD
HHHCCCHHHHHEEEEECCCCEEEEEEEECCCCCCCCEEEEEEEECCCHHHHHHHCCCCHH
IKEAMARSTEQGMQTFDQALFNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKSFNIQD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCEEECCCCCCCEEEEEECCCCCCCEEC
ENLQIQPDDIDKV
CCEEECCCCCCCC
>Mature Secondary Structure
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKSNQMAEIVLSIMNDAQQ
CCCCHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
KEFESKMELNMAFSLAGVGRFRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVL
HHHHHHHHHHHEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIEDPIEYIHQHKKSIVDQREIGI
HHCCEEEEEECCCCCCCHHHEEHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCC
DTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
CEEHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDD
HHHCCCHHHHHEEEEECCCCEEEEEEEECCCCCCCCEEEEEEEECCCHHHHHHHCCCCHH
IKEAMARSTEQGMQTFDQALFNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKSFNIQD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCEEECCCCCCCEEEEEECCCCCCCEEC
ENLQIQPDDIDKV
CCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]