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The map label for this gene is murI [H]

Identifier: 54295149

GI number: 54295149

Start: 2545360

End: 2546199

Strand: Reverse

Name: murI [H]

Synonym: lpl2229

Alternate gene names: 54295149

Gene position: 2546199-2545360 (Counterclockwise)

Preceding gene: 54295150

Following gene: 54295143

Centisome position: 76.1

GC content: 42.5

Gene sequence:

>840_bases
ATGACAAGAAGGAGTAATCAATTGAACTCTAACAAGGATTTGGCTATTGGTGTATTCGATTCAGGCATGGGGGGACTTAC
GGTTTTAAGGGCTCTGCGGGATAAATTACCTCATGAATCTTTTGTTTATTTGGGAGATACCGCCCGTTTACCTTATGGCA
CAAAAAGCCCTGACACAGTCAAACAATATGCTATGCAAATGGCAAAAATTTTGGTTGAGCGCCGCATAAAAGCTTTAGTC
ATTGCCTGTAATACTGCAACGACGGCTGCCTTACCTCATTTACAGAGCATGTTAACCGATATTCCGGTTCTGGGTGTGGT
GGCACCTGGTGCAGCCGCTGCGGTGTCAGCTACCAAAAATCATCGTATTGCTGTTCTGGCTACAGAAACAACCATTGCTT
CCAGAGCCTATCATGATTTGATCATAAAGAATTTGCCTGAGGCAGTTGTCAGCACTCGGGCATGTAGTGTTTTAGTCGCC
TTGGCAGAGGAAGGTATGGTGACTAATGCAGTTGCTCGTGAGGCATTGAAGCATTATCTCGACGATTTTTCGGATGAAGA
TGCTGTGTTATTGGGGTGCACTCACTTTCCAGTATTTAAACCCTTACTATCCAGCTTATTACCAAAAGATGTCGCAATTG
TCGATTCTGCTCAGGCGACTGCAATTTCTCTTTATGAATTATTGCAAAATCAAAATCTGCGCAATGACAATTTGGAAGTT
CCCCCCAGAATCAACTATTTGGTAACAGATTCTATCAATAGGTTCCAGAAGGTAGGCGAGATATTTTTAGGTGAGCAATT
GAGTTTGAGTGATATTGAATTGATTGATGCTATGGGTTAA

Upstream 100 bases:

>100_bases
AAAGTCCGAGTACAGGGATGCGTAAGTAAATAATAATGACTTTGGCTCTCATTTTTGGTAGTACGAAGCTATTTTGCCAA
ATAAGAGAGCCAGGAAATAA

Downstream 100 bases:

>100_bases
TGTCGACAATCGACTAGTATCACCTGCGTCCTGGTGTCCTGGTGCACTAAACCTAATTTAATATGATTCTTTGCAAGGGC
AAAGGTTATTCTTCCTTGGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTRRSNQLNSNKDLAIGVFDSGMGGLTVLRALRDKLPHESFVYLGDTARLPYGTKSPDTVKQYAMQMAKILVERRIKALV
IACNTATTAALPHLQSMLTDIPVLGVVAPGAAAAVSATKNHRIAVLATETTIASRAYHDLIIKNLPEAVVSTRACSVLVA
LAEEGMVTNAVAREALKHYLDDFSDEDAVLLGCTHFPVFKPLLSSLLPKDVAIVDSAQATAISLYELLQNQNLRNDNLEV
PPRINYLVTDSINRFQKVGEIFLGEQLSLSDIELIDAMG

Sequences:

>Translated_279_residues
MTRRSNQLNSNKDLAIGVFDSGMGGLTVLRALRDKLPHESFVYLGDTARLPYGTKSPDTVKQYAMQMAKILVERRIKALV
IACNTATTAALPHLQSMLTDIPVLGVVAPGAAAAVSATKNHRIAVLATETTIASRAYHDLIIKNLPEAVVSTRACSVLVA
LAEEGMVTNAVAREALKHYLDDFSDEDAVLLGCTHFPVFKPLLSSLLPKDVAIVDSAQATAISLYELLQNQNLRNDNLEV
PPRINYLVTDSINRFQKVGEIFLGEQLSLSDIELIDAMG
>Mature_278_residues
TRRSNQLNSNKDLAIGVFDSGMGGLTVLRALRDKLPHESFVYLGDTARLPYGTKSPDTVKQYAMQMAKILVERRIKALVI
ACNTATTAALPHLQSMLTDIPVLGVVAPGAAAAVSATKNHRIAVLATETTIASRAYHDLIIKNLPEAVVSTRACSVLVAL
AEEGMVTNAVAREALKHYLDDFSDEDAVLLGCTHFPVFKPLLSSLLPKDVAIVDSAQATAISLYELLQNQNLRNDNLEVP
PRINYLVTDSINRFQKVGEIFLGEQLSLSDIELIDAMG

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=219, Percent_Identity=36.0730593607306, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 30254; Mature: 30123

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00923 ASP_GLU_RACEMASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRSNQLNSNKDLAIGVFDSGMGGLTVLRALRDKLPHESFVYLGDTARLPYGTKSPDTV
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHH
KQYAMQMAKILVERRIKALVIACNTATTAALPHLQSMLTDIPVLGVVAPGAAAAVSATKN
HHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCC
HRIAVLATETTIASRAYHDLIIKNLPEAVVSTRACSVLVALAEEGMVTNAVAREALKHYL
CEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DDFSDEDAVLLGCTHFPVFKPLLSSLLPKDVAIVDSAQATAISLYELLQNQNLRNDNLEV
HCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCC
PPRINYLVTDSINRFQKVGEIFLGEQLSLSDIELIDAMG
CCCCCEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TRRSNQLNSNKDLAIGVFDSGMGGLTVLRALRDKLPHESFVYLGDTARLPYGTKSPDTV
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHH
KQYAMQMAKILVERRIKALVIACNTATTAALPHLQSMLTDIPVLGVVAPGAAAAVSATKN
HHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCC
HRIAVLATETTIASRAYHDLIIKNLPEAVVSTRACSVLVALAEEGMVTNAVAREALKHYL
CEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DDFSDEDAVLLGCTHFPVFKPLLSSLLPKDVAIVDSAQATAISLYELLQNQNLRNDNLEV
HCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCC
PPRINYLVTDSINRFQKVGEIFLGEQLSLSDIELIDAMG
CCCCCEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA