Click here to switch to the map view.

The map label for this gene is metK [H]

Identifier: 54294920

GI number: 54294920

Start: 2239685

End: 2240833

Strand: Reverse

Name: metK [H]

Synonym: lpl1999

Alternate gene names: 54294920

Gene position: 2240833-2239685 (Counterclockwise)

Preceding gene: 54294921

Following gene: 54294919

Centisome position: 66.98

GC content: 41.86

Gene sequence:

>1149_bases
ATGAATGAAGTCTATGTTTTTACCTCAGAATCTGTTTCCGAGGGCCATCCTGATAAGATAGCTGATCAAATTTCAGATGC
CATTTTAGATGCTATTTTAGCTCAAGACCCAAAAGCACGAGTGGCTTGTGAGGTATTGGTTAAAACCGGTATGGTTTTGG
TTGGTGGAGAAATTACAACAAAAGCCTGGGTAGATGTTGAAGATATAACCCGTCATGTTATAAAGGACATTGGCTATAAT
AGTTCCCAGATGGGGTTTGATTGGGAATCCTGTGCTGTGCTTTCAGCGATTGGCAAGCAATCACCAGACATAGCTCAAGG
AGTAGATAATCAGCAAACTAAAATTCTGGGTGCTGGCGATCAGGGACTTATGTTTGGTTATGCCAGCCGTGAAACAGATG
TTTTTATGCCTGCCCCTATTGCCTATGCGCATCGTTTGATGGAGAAATTAGCTAAGGCTCGTAAATCAGGCCAATTGCCC
TGGTTAAGACCTGATGCAAAATGTCAACTTACTTTGAAATATGAACAAGGGATGCCGGTTGAAGTGGATACTGTTGTTTT
TTCTACGCAGCATTCACCTGATATTGAGCATAAAGATTTGGTTGAGGCGATCCGTGAAGAAATTATTAAATCAGTTCTTC
CTGCTGAATGGCTCAATGATAAGACCCGGTATTTCATTAATCCAACAGGACGCTTCGTAATTGGTGGTCCTTTGGGCGAT
TGTGGCTTAACTGGCCGTAAAATCATTGTTGATACTTATGGTGGTATGGCAAGACATGGCGGAGGCTGTTTTTCAGGTAA
AGATCCTTCGAAGGTAGATCGCTCCGCAGCATACGCCGCAAGACATGTTGCCAAGAATATTGTGGCGGCTGGTTTAGCGG
ATAAATGCGAGTTACAGATTTCCTATGCTATTGGTGTTGCTGAACCTACATCTATTTTTGTTGATACTTTTGGTACTGGA
CGACTAAAAAACAGCGAAATAATTGATTTAATTCATACGCATTTTGATTTAACGCCGCAAGGTATTATTGATCAACACGA
TTTGCTTCGTCCTATTTATAGGCAGACGGCAACTTATGGGCACTATGGCAGGGAAAGTTTTCCATGGGAGCGATTGGACA
AAGTAGCTGAATTAAGCAAGGCATTATAA

Upstream 100 bases:

>100_bases
ATGACATGGATTTACCAAAACAATGACTTTAACTTGTTTTGTGTTTTAAGCAGAGATAAAGATTAGGGATACTAAAAATA
GAAAATACAGAGGGTATGTA

Downstream 100 bases:

>100_bases
TTTTTTAAGGATACTCTAATGGAACTGGTAGAAGAAGGAGTACAGAAAATCAATACATTACAAGATTATAAAGTAGCAGA
TATTTCATTAGCGGACTGGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITTKAWVDVEDITRHVIKDIGYN
SSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTG
RLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYGHYGRESFPWERLDKVAELSKAL

Sequences:

>Translated_382_residues
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITTKAWVDVEDITRHVIKDIGYN
SSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTG
RLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYGHYGRESFPWERLDKVAELSKAL
>Mature_382_residues
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITTKAWVDVEDITRHVIKDIGYN
SSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTG
RLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYGHYGRESFPWERLDKVAELSKAL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=56.7204301075269, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI5174529, Length=371, Percent_Identity=55.256064690027, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI1789311, Length=377, Percent_Identity=71.6180371352785, Blast_Score=570, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI17538498, Length=372, Percent_Identity=57.258064516129, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17538494, Length=372, Percent_Identity=56.989247311828, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25145633, Length=376, Percent_Identity=55.5851063829787, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=54.5219638242894, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=372, Percent_Identity=51.0752688172043, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=54.601226993865, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI25145635, Length=330, Percent_Identity=53.3333333333333, Blast_Score=340, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=53.8461538461538, Blast_Score=311, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=64.6341463414634, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=69.5652173913043, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=376, Percent_Identity=56.3829787234042, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=55.4089709762533, Blast_Score=415, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580527, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580525, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45552159, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580541, Length=358, Percent_Identity=53.9106145251397, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=57.9831932773109, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41965; Mature: 41965

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITT
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCC
KAWVDVEDITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGD
EEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCEEEEECCC
QGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLPWLRPDAKCQLTLKYEQGMPV
CCEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCE
EVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQI
CCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYG
EEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
HYGRESFPWERLDKVAELSKAL
CCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITT
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCC
KAWVDVEDITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGD
EEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCEEEEECCC
QGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLPWLRPDAKCQLTLKYEQGMPV
CCEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCE
EVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQI
CCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYG
EEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
HYGRESFPWERLDKVAELSKAL
CCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA