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The map label for this gene is spoT [H]

Identifier: 54294906

GI number: 54294906

Start: 2225296

End: 2227419

Strand: Reverse

Name: spoT [H]

Synonym: lpl1985

Alternate gene names: 54294906

Gene position: 2227419-2225296 (Counterclockwise)

Preceding gene: 54294907

Following gene: 54294905

Centisome position: 66.58

GC content: 41.29

Gene sequence:

>2124_bases
GTGAGCTACTTTAAAGAGCTCGATGAAGAGCTTAAATGCTACCTTGAGCAGGCACAAATAGAGAAATGTTATCAAGCTTA
TTTGGTGGCTGAAAAAGCACACCATGGCCAAATGCGTCGTTCAGGTGAGCCTTATATTACACACCCTGTAGCAGCAGCCC
TCATTCTGGCAAGAATGAGGCTGGACTATCAAACTATCATGGCCACTCTGCTTCATGACGTCGTTGAAGATACCTCCATA
AGTAAAGAAGAACTGGTTAATCAATTTGGTGAAGAAGTCACTGCCCTTGTTGATGGTGTCACCAAGCTCACCAAAATTAA
ATTTGAATCAAAGGCCGAAGCTCAAGCGGAAAATTTTCGCAAGATGGTTTTGGCTATGGTTAAGGACATTCGCGTTATTA
TCGTTAAATTGGCTGATCGCTTACATAATATGCGCACTTTAGGATCAATGCCCGCAGCCAAGCGCAGACGAATTGCAATT
GAAACGCTCGAGATTTATGCCCCTATTGCTAATCGTTTGGGTATGCATTCTATTTATATCGGCCTTGAGGATTTGGGTTT
TCAAGCTTTATATCCTATGCGTTACAGGGCAATTAAGTCTGCTGTTGAAAAATCTCGGGGAAATCGAAGAGAGTTGACAC
AGACTATAGAGAAAGATTTGCAACACGCCTTATCGCAATTGAACATTCCTTATGAACAAGTATTTGGCAGGCAAAAGCAT
TTATATAGTATTTATAGAAAAATGAAACAGAAAAAAGCATCCTTTACAGAAATTACGGATGTGTTCGCATTTCGCATCAT
TACAGAAGACATTGACTCCTGTTATAGAATTTTGGGAGCTTTACACTGTACTTATAAACCTGTCCCACAACGATTTAAAG
ATTATATCGGAATTCCCAAAGCCAATGGCTATCAGTCATTGCATACCACTTTGTTTGGGCCTTTTGGTGTTCCTCTTGAG
GTTCAAATACGCACAAGAGAGATGGATAAAGTAGCTGATAATGGTGTAGCAGCGCACTGGATCTATAAATCTTCCGGATT
GGAAGTCAATGAAGCTCAACTTCGTGCCAGAGAGTGGGTTCAAAGTTTGCTAGAAATGCAAAGGAGCACGGGCAGTTCTC
TTGAATTTATCGAAAACGTTAAAATAGATTTATTTCCTGATGAAGTATATGTTTTTACTCCCAAAGGGCACATTATGGAG
TTGCCGAAAGGAGCCACTCCTGTAGATTTTGCATACACGGTACACTCTGGAGTGGGAAATAGTTGTGTTGCAGCGAAAGT
GAACAGGCGTTTGGTTCCTTTAAGCATTCCTTTATCCAATGGTCAAACCGTAGAGATTATTACAGCCCCGGGAGCACACC
CCAACCCGGCTTGGCTGAATTTTGTTGTGACTGGAAAAGCAAGATCAAATATTCGTCATTTTCTGAAAAGCCAACAACAC
GCTGAGTCTATTGTTTTAGGCAAACGATTATTGGAGCAATCATTGGCTGAATTATCCAGTGATTACGCCAAAGTGCCTCC
GGAAACATTACAGGCATTGCTCAGCGATTTACACTATAAAACGGTTGATGACTTATTGTATGCCATAGGAATAGGTAATC
AGATGGCAATGGTTATTGCCAAGCGGTTGGTAGTTTCGCAAGACGCCAATGAATCGGATAAGGGCAATAAAAAACCTTTG
GCTATTAAAGGCACTGAAGGGATGGTTGTGCATTTTGGTGAATGCTGCCAACCAATACCTGGAGATAATATAGTTGGTAA
ATTTCAACAAGGCAGAGGCATTATTGTTCATGCCAGTGACTGCACTACACTAAAAACAACTCGTAGTAATCCGGAACAGT
TTATTGCTTTGCGTTGGGATGAGCAGGTTCAGGGTGAATATTGGGTAGACATTACTGTAGATGTAGCCAACGAAAGAGGT
GTATTGGCTGCTTTAGCAACTGCTATTTCAGAGGCAGACTCTAATATAGGGAATATTAATGTGGATCCTAGGGATGGTCG
TCATAATGCGGTCACTTTTTCCATCAGTGTTCGTGACAGAACCCATTTGGCTCGAGTCATGCGTCGATTAAGAGCAAATA
AAGTAGTTATGCGGTTATATCGAAAAAAACAAGGGGAGATTTAA

Upstream 100 bases:

>100_bases
TGGTATTTAAGCAAAGCAAAGGGAACAAGGGCGAGGCATAAAACCGTATTTTGAGTAATGTGAGGTATAAATAGCCATGT
TGGATACTAGGGAGTACTGC

Downstream 100 bases:

>100_bases
TGCAACCAATCAATACAAAATTAGCGCCAAAAGCGATAGGCACGTACAGTCAGGCGATAAAAAGTGGTGAGATCGTTTTT
CTTTCGGGGCAGATCCCATT

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 707; Mature: 706

Protein sequence:

>707_residues
MSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEPYITHPVAAALILARMRLDYQTIMATLLHDVVEDTSI
SKEELVNQFGEEVTALVDGVTKLTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAI
ETLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEKDLQHALSQLNIPYEQVFGRQKH
LYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYRILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLFGPFGVPLE
VQIRTREMDKVADNGVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVFTPKGHIME
LPKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAPGAHPNPAWLNFVVTGKARSNIRHFLKSQQH
AESIVLGKRLLEQSLAELSSDYAKVPPETLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANESDKGNKKPL
AIKGTEGMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQGEYWVDITVDVANERG
VLAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTHLARVMRRLRANKVVMRLYRKKQGEI

Sequences:

>Translated_707_residues
MSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEPYITHPVAAALILARMRLDYQTIMATLLHDVVEDTSI
SKEELVNQFGEEVTALVDGVTKLTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAI
ETLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEKDLQHALSQLNIPYEQVFGRQKH
LYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYRILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLFGPFGVPLE
VQIRTREMDKVADNGVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVFTPKGHIME
LPKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAPGAHPNPAWLNFVVTGKARSNIRHFLKSQQH
AESIVLGKRLLEQSLAELSSDYAKVPPETLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANESDKGNKKPL
AIKGTEGMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQGEYWVDITVDVANERG
VLAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTHLARVMRRLRANKVVMRLYRKKQGEI
>Mature_706_residues
SYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEPYITHPVAAALILARMRLDYQTIMATLLHDVVEDTSIS
KEELVNQFGEEVTALVDGVTKLTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAIE
TLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEKDLQHALSQLNIPYEQVFGRQKHL
YSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYRILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLFGPFGVPLEV
QIRTREMDKVADNGVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVFTPKGHIMEL
PKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAPGAHPNPAWLNFVVTGKARSNIRHFLKSQQHA
ESIVLGKRLLEQSLAELSSDYAKVPPETLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANESDKGNKKPLA
IKGTEGMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQGEYWVDITVDVANERGV
LAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTHLARVMRRLRANKVVMRLYRKKQGEI

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=703, Percent_Identity=53.0583214793741, Blast_Score=786, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=659, Percent_Identity=35.660091047041, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79692; Mature: 79561

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEPYITHPVAAALILARMR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEHHHHHHHHHHHHH
LDYQTIMATLLHDVVEDTSISKEELVNQFGEEVTALVDGVTKLTKIKFESKAEAQAENFR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAIETLEIYAPIANRLGMHSIYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEKDLQHALSQLNIPYEQVFGRQKH
EHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHH
LYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYRILGALHCTYKPVPQRFKDYIGIPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCC
ANGYQSLHTTLFGPFGVPLEVQIRTREMDKVADNGVAAHWIYKSSGLEVNEAQLRAREWV
CCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
QSLLEMQRSTGSSLEFIENVKIDLFPDEVYVFTPKGHIMELPKGATPVDFAYTVHSGVGN
HHHHHHHHCCCCCHHHHHCCEEEEECCCEEEECCCCCEEECCCCCCCCEEEEEECCCCCC
SCVAAKVNRRLVPLSIPLSNGQTVEIITAPGAHPNPAWLNFVVTGKARSNIRHFLKSQQH
CHHHHHHCCEEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
AESIVLGKRLLEQSLAELSSDYAKVPPETLQALLSDLHYKTVDDLLYAIGIGNQMAMVIA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHH
KRLVVSQDANESDKGNKKPLAIKGTEGMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASD
HHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEHHHCCCCCCCCCHHHHCCCCEEEEECCC
CTTLKTTRSNPEQFIALRWDEQVQGEYWVDITVDVANERGVLAALATAISEADSNIGNIN
CCEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEE
VDPRDGRHNAVTFSISVRDRTHLARVMRRLRANKVVMRLYRKKQGEI
CCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEPYITHPVAAALILARMR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEHHHHHHHHHHHHH
LDYQTIMATLLHDVVEDTSISKEELVNQFGEEVTALVDGVTKLTKIKFESKAEAQAENFR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAIETLEIYAPIANRLGMHSIYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEKDLQHALSQLNIPYEQVFGRQKH
EHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHH
LYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYRILGALHCTYKPVPQRFKDYIGIPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCC
ANGYQSLHTTLFGPFGVPLEVQIRTREMDKVADNGVAAHWIYKSSGLEVNEAQLRAREWV
CCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
QSLLEMQRSTGSSLEFIENVKIDLFPDEVYVFTPKGHIMELPKGATPVDFAYTVHSGVGN
HHHHHHHHCCCCCHHHHHCCEEEEECCCEEEECCCCCEEECCCCCCCCEEEEEECCCCCC
SCVAAKVNRRLVPLSIPLSNGQTVEIITAPGAHPNPAWLNFVVTGKARSNIRHFLKSQQH
CHHHHHHCCEEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
AESIVLGKRLLEQSLAELSSDYAKVPPETLQALLSDLHYKTVDDLLYAIGIGNQMAMVIA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHH
KRLVVSQDANESDKGNKKPLAIKGTEGMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASD
HHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEHHHCCCCCCCCCHHHHCCCCEEEEECCC
CTTLKTTRSNPEQFIALRWDEQVQGEYWVDITVDVANERGVLAALATAISEADSNIGNIN
CCEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEE
VDPRDGRHNAVTFSISVRDRTHLARVMRRLRANKVVMRLYRKKQGEI
CCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]