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The map label for this gene is pksJ [H]

Identifier: 54294831

GI number: 54294831

Start: 2140704

End: 2142020

Strand: Reverse

Name: pksJ [H]

Synonym: lpl1909

Alternate gene names: 54294831

Gene position: 2142020-2140704 (Counterclockwise)

Preceding gene: 54294832

Following gene: 54294829

Centisome position: 64.02

GC content: 40.47

Gene sequence:

>1317_bases
ATGAGTGACAATAACTACTCAAACAAAATTGCTATTGTTGGTATGGCTGTAAAATTTCCTGGGGCTAATGATCTTGATGA
ATATTGGGATTTATTGAAGTTTGGTAAAGAAGCAGTCACTAAATTTTCCTCAGAACAATTACAGCGGTCAGGTATTTCAG
AGCAATTAATCGCTAATCCCTATTATAAACCATACAGAGGGATATTAGATGACCTGGAGTCATTCGATAGCGCTCCGTTT
GAAAATACCACCAAAAATTTTGAATTGTTGGGTGTGCAAGGCAAAGTGATGTCGCATTTAACTTATCGGGCTCTGAGCAC
CATGAGAAGTCAGGAGCATCATTCAAATAACTACATAAAAAATACGGCTGTTTATATTGGCGCAAATAATCAACCCGCCT
CCCATTTCCTGGGAGCCGCCTACTACCAGGCAATTGATACTCAAAAAGCGATAGAAAGGTATTATTCAAAAGTCATTGCA
CCATATATTTCTTATCAATTTGGATTTCAGGGTAGTTCTATCGATTTATATACAGCTTGCTCGTCTTCTCTGACAGCCGT
GATCCAAAGCTGTAGAGAGTTAAACAGTCATCAATGTGATATGGCAATTGCAGGTGCGTGCTTAATCGATTTACCCCAGG
AAGTTGGATATCTTTATGAAGAGAATGGCTTGTTTTCCTCCGATGGACATTGCCATTCTTTTGATGTTTCGGCATGTGGC
ACCTTATATTCAAACGGTGCTGGATTGGTTGTTCTAAAGCGGCTTGAAGATGCCATTGCGGATAATGATCTAATCCATGC
TGTGATTATCGGCTCCGCCATGAATAACGATGGTAATTCTTCAAAAGAAGGATTTATGGCACCGGGGATCCATGGCCAAT
ATACTTGCTTACAGTCAGCTTGGAAAAACGCGAGGGTTGAGCCAGGCGATCTGGATTATATTGCAGCTCATGGTGCTTCA
ACCAAGCTGGGTGATGCAACAGAAATATATGCCTTAAAAAAAGCGTTCAAAGACGTTAAAAAGACACATTGTTGTCCAAT
AAGCTCAGTAATTAGTAATCTGGGGCATACTGGCATTGTGTCCGGTATGGCGTCAATCATTAAAACTGTGCTTATGCTCA
AAAATAAGGCTGTGGTCCCTTCAATTAATTTTGAAACCCCTAACCCGGATTTTATGTTGGAAGACTCGCCTATATATATT
GCCACTCAATTTCAGGATTTCCCTCAAATTAAAAAACACTATTTGGCCGGTGTTTCCAGTTATGGCGCAGGGGGAAGTAA
CACACATCTTGTTTTGCGTAGCTATGATGAAAGCTAG

Upstream 100 bases:

>100_bases
AAATAATATAAACTATGCTTTATGTTATGAACCCGTCGTACGTCGTCTAGTTAGCGTTTATTAAATTCTTGGTGCGGCCA
AAGAAATGGAGCCATAAATT

Downstream 100 bases:

>100_bases
GGTTCTGTTGATATTTTGTTTTTATTCCTGTTAAAAGTTTACCTGATGCCGATTGCTCGTTAGAGAGCTATGTAGAATTT
AAGCAGCGATGAACATGTCA

Product: hypothetical protein

Products: L-tryptophan [Cytoplasm]; L-tyrosine [Cytoplasm]; Proton [Cytoplasm]; L-phenylalanine [Cytoplasm] [C]

Alternate protein names: PKS [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSSEQLQRSGISEQLIANPYYKPYRGILDDLESFDSAPF
ENTTKNFELLGVQGKVMSHLTYRALSTMRSQEHHSNNYIKNTAVYIGANNQPASHFLGAAYYQAIDTQKAIERYYSKVIA
PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDVSACG
TLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNNDGNSSKEGFMAPGIHGQYTCLQSAWKNARVEPGDLDYIAAHGAS
TKLGDATEIYALKKAFKDVKKTHCCPISSVISNLGHTGIVSGMASIIKTVLMLKNKAVVPSINFETPNPDFMLEDSPIYI
ATQFQDFPQIKKHYLAGVSSYGAGGSNTHLVLRSYDES

Sequences:

>Translated_438_residues
MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSSEQLQRSGISEQLIANPYYKPYRGILDDLESFDSAPF
ENTTKNFELLGVQGKVMSHLTYRALSTMRSQEHHSNNYIKNTAVYIGANNQPASHFLGAAYYQAIDTQKAIERYYSKVIA
PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDVSACG
TLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNNDGNSSKEGFMAPGIHGQYTCLQSAWKNARVEPGDLDYIAAHGAS
TKLGDATEIYALKKAFKDVKKTHCCPISSVISNLGHTGIVSGMASIIKTVLMLKNKAVVPSINFETPNPDFMLEDSPIYI
ATQFQDFPQIKKHYLAGVSSYGAGGSNTHLVLRSYDES
>Mature_437_residues
SDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSSEQLQRSGISEQLIANPYYKPYRGILDDLESFDSAPFE
NTTKNFELLGVQGKVMSHLTYRALSTMRSQEHHSNNYIKNTAVYIGANNQPASHFLGAAYYQAIDTQKAIERYYSKVIAP
YISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDVSACGT
LYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNNDGNSSKEGFMAPGIHGQYTCLQSAWKNARVEPGDLDYIAAHGAST
KLGDATEIYALKKAFKDVKKTHCCPISSVISNLGHTGIVSGMASIIKTVLMLKNKAVVPSINFETPNPDFMLEDSPIYIA
TQFQDFPQIKKHYLAGVSSYGAGGSNTHLVLRSYDES

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism [H]

COG id: COG3321

COG function: function code Q; Polyketide synthase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI41872631, Length=441, Percent_Identity=28.5714285714286, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI8923559, Length=289, Percent_Identity=26.643598615917, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI223972627, Length=145, Percent_Identity=31.0344827586207, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788663, Length=313, Percent_Identity=25.2396166134185, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787337, Length=284, Percent_Identity=25.7042253521127, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17550940, Length=445, Percent_Identity=27.6404494382022, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI212642053, Length=435, Percent_Identity=27.5862068965517, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71983541, Length=293, Percent_Identity=25.2559726962457, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71983535, Length=266, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320904, Length=296, Percent_Identity=25.3378378378378, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI221330659, Length=439, Percent_Identity=28.4738041002278, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19920632, Length=439, Percent_Identity=28.4738041002278, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24581345, Length=433, Percent_Identity=26.0969976905312, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24644341, Length=462, Percent_Identity=23.8095238095238, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 24024 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 30,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR000794
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020842
- InterPro:   IPR020841
- InterPro:   IPR020807
- InterPro:   IPR013968
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF08659 KR; PF00550 PP-binding [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 47945; Mature: 47814

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSSEQLQRSGISEQLIANP
CCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
YYKPYRGILDDLESFDSAPFENTTKNFELLGVQGKVMSHLTYRALSTMRSQEHHSNNYIK
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEE
NTAVYIGANNQPASHFLGAAYYQAIDTQKAIERYYSKVIAPYISYQFGFQGSSIDLYTAC
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEHHH
SSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDVSACG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHEECHHHHCCEEECCCCCCCCCCCEEECHHHHH
TLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNNDGNSSKEGFMAPGIHGQYTCLQSA
HHHCCCCCEEEHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
WKNARVEPGDLDYIAAHGASTKLGDATEIYALKKAFKDVKKTHCCPISSVISNLGHTGIV
HHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHH
SGMASIIKTVLMLKNKAVVPSINFETPNPDFMLEDSPIYIATQFQDFPQIKKHYLAGVSS
HHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHHH
YGAGGSNTHLVLRSYDES
CCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSSEQLQRSGISEQLIANP
CCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
YYKPYRGILDDLESFDSAPFENTTKNFELLGVQGKVMSHLTYRALSTMRSQEHHSNNYIK
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEE
NTAVYIGANNQPASHFLGAAYYQAIDTQKAIERYYSKVIAPYISYQFGFQGSSIDLYTAC
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEHHH
SSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDVSACG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHEECHHHHCCEEECCCCCCCCCCCEEECHHHHH
TLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNNDGNSSKEGFMAPGIHGQYTCLQSA
HHHCCCCCEEEHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
WKNARVEPGDLDYIAAHGASTKLGDATEIYALKKAFKDVKKTHCCPISSVISNLGHTGIV
HHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHH
SGMASIIKTVLMLKNKAVVPSINFETPNPDFMLEDSPIYIATQFQDFPQIKKHYLAGVSS
HHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHHH
YGAGGSNTHLVLRSYDES
CCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: L-tryptophan [Periplasm]; L-tyrosine [Periplasm]; Proton [Periplasm]; L-phenylalanine [Periplasm] [C]

Specific reaction: acyl-ACP + malonyl-ACP = beta-keto-enoyl-ACP + [acyl carrier protein] + CO2 acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 malonyl-ACP = acetyl-ACP + CO2

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377; 10568751 [H]