BacMap

Definition	Legionella pneumophila str. Lens, complete genome.
Accession	NC_006369
Length	3,345,687

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The map label for this gene is mycC [H]

Identifier: 54294832

GI number: 54294832

Start: 2142149

End: 2143714

Strand: Reverse

Name: mycC [H]

Synonym: lpl1910

Alternate gene names: 54294832

Gene position: 2143714-2142149 (Counterclockwise)

Preceding gene: 54294833

Following gene: 54294831

Centisome position: 64.07

GC content: 39.14

Gene sequence:

>1566_bases
ATGCGATCCCCTGATCAGTACACGACAATTTGCTCCTTATTTGAGGAGAAGGTAAAGCAATGCCTGGAAAATACAGCGGT
TTATCTGGAAGATCGCCAAATTTCGTATGATGCCTTTAATAAACAAGCCAATCAATACGCGCGGTATCTGCAAAGAAAAG
GGTTGCCATTAAATGGAATTGTCGGTATTCAACTCGCGCGCTCTTTTGAAATGCTGGTAGCTATTTTTGCAGTATTAAAG
GCAGGTGGTGCCTATTTGCCGATAGATCCCATGGCCCCTGTTCTGAGAAATCAAGCCATCCTTGAAGACAGCCAAATCAA
ATTTCTAATTGTCAGCGATCATTCATTATTGACGGCAACCCCGGGTTTGCAAGTGTTAAGCATTCATGCCAATGTGAGCG
ATGAAGATTGCCATAATTTAAACCTGCCCGGGAAAAAACAGGATTTAGCGTATGTTATGTATACTTCAGGCACTACTGGC
AAGCCGAAAGGAGTCCTGATTTCACATCAAGCTTTGCTTAATCGTATTTTATGGATGCAAAACACATTTCCCTTGCAAAG
TCATGATGTAGTATTTCATAAGACACATATTTGTTTTGACATCTCTGTCTGGGAGACCTGTTGGTGGTCTATCGCGGGCG
CCGGAGTAGTACTTTTACCGCCAAGAAAAGAACATGACATCAAGCTGTTTATTCAAATGATTGAGCAATATCAAATCAAT
GTCATTCATTTTGTACCCTCCGTATTACGAATATTTTTAAGTTACATCGAACAGGATTTTTCGATAAATCGCTTAAGCTC
TTTAAAATACGTATTTTCAAGTGGAGAGGCGCTGGATGCCAAAAGTGTTAATCTATTCAACCAGTTATTTCAAGATCAAA
AAACAATATTAGTCAATTTATACGGGCCCACAGAGGCCACAATAGATGTTTCCTGTTTTATTTGTGAAAAGCACAAACGT
TTTAACACTGTACCCATTGGAAAGCCTATCCAAAATACTCAATTTTTTGTGTTAGACGAGCAGTTAAATTCAAGCGGCTT
CGAACCTGGAGAGCTTTATATTTCCGGAGTTGGATTAGCTCAGGGGTATTTAAATAATCCAGTGCTGACTCAGGCATCGT
TTATAGACAATCCTTACTTGCCTGGAGAAAAAATGTATCGTACTGGAGATATAGTCCGTTGGAATCAAGAGGGTGAGTTA
TTATTTTTAGGCCGCAAGGACGATCAGGTCAAATTACGTGGCATACGTATTGAATTAGGAGAAATTCAGCATCATTTATT
AGCGCATCCCAACATCCATGATGCTGTCGTGGTCTGTGAGAAAGTAGATTGTCTTGATCATAGAATGGTTGTATTCCTGA
TTGCTAATAACAAACAAACAAAGATTGGCAGCGCAGAACTGAAATCCTTTTTAAAAAACAGGCTCCCGGATTATATGATA
CCAGAGCATTATCTATGGTTAAGTGCATTTCCAATAAAAGAAAATGGAAAAATAGACAAAGAGACACTATTGAGTTTCAT
TGAGCATGAGCAATGCCCGAGAAATAAAAATAATCAGGGAAAATGA

Upstream 100 bases:

>100_bases
AGAGATGCCAAGGTAATGGAGATCATTGAAGGTACCTCACAAATGCACGAGATACTCATTTCAAAGCTTTGCAACAATAA
ATAGAGGATATTATTAAACA

Downstream 100 bases:

>100_bases
CTTATACTTAATTAAAAGCCGGTTGCAGAAATAATATAAACTATGCTTTATGTTATGAACCCGTCGTACGTCGTCTAGTT
AGCGTTTATTAAATTCTTGG

Product: hypothetical protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent serine adenylase; SerA; Serine activase; ATP-dependent asparagine adenylase 3; AsnA 3; Asparagine activase 3 [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MRSPDQYTTICSLFEEKVKQCLENTAVYLEDRQISYDAFNKQANQYARYLQRKGLPLNGIVGIQLARSFEMLVAIFAVLK
AGGAYLPIDPMAPVLRNQAILEDSQIKFLIVSDHSLLTATPGLQVLSIHANVSDEDCHNLNLPGKKQDLAYVMYTSGTTG
KPKGVLISHQALLNRILWMQNTFPLQSHDVVFHKTHICFDISVWETCWWSIAGAGVVLLPPRKEHDIKLFIQMIEQYQIN
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNQLFQDQKTILVNLYGPTEATIDVSCFICEKHKR
FNTVPIGKPIQNTQFFVLDEQLNSSGFEPGELYISGVGLAQGYLNNPVLTQASFIDNPYLPGEKMYRTGDIVRWNQEGEL
LFLGRKDDQVKLRGIRIELGEIQHHLLAHPNIHDAVVVCEKVDCLDHRMVVFLIANNKQTKIGSAELKSFLKNRLPDYMI
PEHYLWLSAFPIKENGKIDKETLLSFIEHEQCPRNKNNQGK

Sequences:

>Translated_521_residues
MRSPDQYTTICSLFEEKVKQCLENTAVYLEDRQISYDAFNKQANQYARYLQRKGLPLNGIVGIQLARSFEMLVAIFAVLK
AGGAYLPIDPMAPVLRNQAILEDSQIKFLIVSDHSLLTATPGLQVLSIHANVSDEDCHNLNLPGKKQDLAYVMYTSGTTG
KPKGVLISHQALLNRILWMQNTFPLQSHDVVFHKTHICFDISVWETCWWSIAGAGVVLLPPRKEHDIKLFIQMIEQYQIN
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNQLFQDQKTILVNLYGPTEATIDVSCFICEKHKR
FNTVPIGKPIQNTQFFVLDEQLNSSGFEPGELYISGVGLAQGYLNNPVLTQASFIDNPYLPGEKMYRTGDIVRWNQEGEL
LFLGRKDDQVKLRGIRIELGEIQHHLLAHPNIHDAVVVCEKVDCLDHRMVVFLIANNKQTKIGSAELKSFLKNRLPDYMI
PEHYLWLSAFPIKENGKIDKETLLSFIEHEQCPRNKNNQGK
>Mature_521_residues
MRSPDQYTTICSLFEEKVKQCLENTAVYLEDRQISYDAFNKQANQYARYLQRKGLPLNGIVGIQLARSFEMLVAIFAVLK
AGGAYLPIDPMAPVLRNQAILEDSQIKFLIVSDHSLLTATPGLQVLSIHANVSDEDCHNLNLPGKKQDLAYVMYTSGTTG
KPKGVLISHQALLNRILWMQNTFPLQSHDVVFHKTHICFDISVWETCWWSIAGAGVVLLPPRKEHDIKLFIQMIEQYQIN
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNQLFQDQKTILVNLYGPTEATIDVSCFICEKHKR
FNTVPIGKPIQNTQFFVLDEQLNSSGFEPGELYISGVGLAQGYLNNPVLTQASFIDNPYLPGEKMYRTGDIVRWNQEGEL
LFLGRKDDQVKLRGIRIELGEIQHHLLAHPNIHDAVVVCEKVDCLDHRMVVFLIANNKQTKIGSAELKSFLKNRLPDYMI
PEHYLWLSAFPIKENGKIDKETLLSFIEHEQCPRNKNNQGK

Specific function: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Ser and Asn as part of the synthesis of mycosubtilin. The Ser residue is further epimerized to the D-isomer form. The activation sites for these amino acids consist

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=474, Percent_Identity=24.2616033755274, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI187761343, Length=474, Percent_Identity=24.2616033755274, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI45580730, Length=363, Percent_Identity=26.7217630853994, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI156151445, Length=568, Percent_Identity=21.830985915493, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI38505220, Length=506, Percent_Identity=21.7391304347826, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI115511026, Length=427, Percent_Identity=25.5269320843091, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI42544132, Length=439, Percent_Identity=23.6902050113895, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI157311624, Length=482, Percent_Identity=21.7842323651452, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI157311622, Length=482, Percent_Identity=21.7842323651452, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI58082049, Length=482, Percent_Identity=21.3692946058091, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI13375727, Length=393, Percent_Identity=19.8473282442748, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786801, Length=509, Percent_Identity=36.9351669941061, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1786810, Length=512, Percent_Identity=23.4375, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI145693145, Length=512, Percent_Identity=24.609375, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788107, Length=553, Percent_Identity=21.6998191681736, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790505, Length=529, Percent_Identity=20.7939508506616, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556356, Length=493, Percent_Identity=26.9776876267748, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17550940, Length=498, Percent_Identity=23.4939759036145, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=353, Percent_Identity=24.929178470255, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25147511, Length=366, Percent_Identity=26.5027322404372, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32564422, Length=361, Percent_Identity=26.8698060941828, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI32564420, Length=361, Percent_Identity=26.8698060941828, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=493, Percent_Identity=21.501014198783, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=373, Percent_Identity=22.2520107238606, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=506, Percent_Identity=21.9367588932806, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=403, Percent_Identity=23.5732009925558, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71994694, Length=387, Percent_Identity=21.9638242894057, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994690, Length=387, Percent_Identity=21.9638242894057, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994703, Length=387, Percent_Identity=21.9638242894057, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=583, Percent_Identity=24.5283018867925, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319699, Length=390, Percent_Identity=26.4102564102564, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648676, Length=514, Percent_Identity=31.7120622568093, Blast_Score=234, Evalue=8e-62,
Organism=Drosophila melanogaster, GI24582852, Length=512, Percent_Identity=24.21875, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24656500, Length=536, Percent_Identity=22.9477611940299, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653035, Length=486, Percent_Identity=22.6337448559671, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581924, Length=366, Percent_Identity=23.4972677595628, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI161076582, Length=487, Percent_Identity=22.3819301848049, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356947, Length=478, Percent_Identity=21.1297071129707, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 59297; Mature: 59297

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSPDQYTTICSLFEEKVKQCLENTAVYLEDRQISYDAFNKQANQYARYLQRKGLPLNGI
CCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCEECHHHHHHHHHHHHHHHHHCCCCCCCE
VGIQLARSFEMLVAIFAVLKAGGAYLPIDPMAPVLRNQAILEDSQIKFLIVSDHSLLTAT
EHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHCCHHCCCCCEEEEEEECCCEEEEC
PGLQVLSIHANVSDEDCHNLNLPGKKQDLAYVMYTSGTTGKPKGVLISHQALLNRILWMQ
CCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHC
NTFPLQSHDVVFHKTHICFDISVWETCWWSIAGAGVVLLPPRKEHDIKLFIQMIEQYQIN
CCCCCCCCCEEEEEEEEEEEEHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCC
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNQLFQDQKTILVNL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEE
YGPTEATIDVSCFICEKHKRFNTVPIGKPIQNTQFFVLDEQLNSSGFEPGELYISGVGLA
ECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEHH
QGYLNNPVLTQASFIDNPYLPGEKMYRTGDIVRWNQEGELLFLGRKDDQVKLRGIRIELG
HHCCCCCCEEEHHHCCCCCCCCCCEEECCCEEEECCCCCEEEEECCCCCEEEEEEEEEHH
EIQHHLLAHPNIHDAVVVCEKVDCLDHRMVVFLIANNKQTKIGSAELKSFLKNRLPDYMI
HHHHHHHCCCCCCCEEEEEEHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCC
PEHYLWLSAFPIKENGKIDKETLLSFIEHEQCPRNKNNQGK
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRSPDQYTTICSLFEEKVKQCLENTAVYLEDRQISYDAFNKQANQYARYLQRKGLPLNGI
CCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCEECHHHHHHHHHHHHHHHHHCCCCCCCE
VGIQLARSFEMLVAIFAVLKAGGAYLPIDPMAPVLRNQAILEDSQIKFLIVSDHSLLTAT
EHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHCCHHCCCCCEEEEEEECCCEEEEC
PGLQVLSIHANVSDEDCHNLNLPGKKQDLAYVMYTSGTTGKPKGVLISHQALLNRILWMQ
CCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHC
NTFPLQSHDVVFHKTHICFDISVWETCWWSIAGAGVVLLPPRKEHDIKLFIQMIEQYQIN
CCCCCCCCCEEEEEEEEEEEEHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCC
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNQLFQDQKTILVNL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEE
YGPTEATIDVSCFICEKHKRFNTVPIGKPIQNTQFFVLDEQLNSSGFEPGELYISGVGLA
ECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEHH
QGYLNNPVLTQASFIDNPYLPGEKMYRTGDIVRWNQEGELLFLGRKDDQVKLRGIRIELG
HHCCCCCCEEEHHHCCCCCCCCCCEEECCCEEEECCCCCEEEEECCCCCEEEEEEEEEHH
EIQHHLLAHPNIHDAVVVCEKVDCLDHRMVVFLIANNKQTKIGSAELKSFLKNRLPDYMI
HHHHHHHCCCCCCCEEEEEEHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCC
PEHYLWLSAFPIKENGKIDKETLLSFIEHEQCPRNKNNQGK
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]