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The map label for this gene is gloB [H]

Identifier: 54294193

GI number: 54294193

Start: 1399396

End: 1400160

Strand: Reverse

Name: gloB [H]

Synonym: lpl1257

Alternate gene names: 54294193

Gene position: 1400160-1399396 (Counterclockwise)

Preceding gene: 54294194

Following gene: 54294192

Centisome position: 41.85

GC content: 37.65

Gene sequence:

>765_bases
ATGACTATCCTGCCAATTTCGGCTTTTTCAGATAATTATATTTGGACCTTTATTGATAAAATAGCTGGCGTATTAGATTG
CGTCGATCCGGGAGAGTCGGCACCAATAATACGCTTTGCCCAATCCAACCAACTGACACTAAGAACGATATTATTAACCC
ATCACCACTATGATCACATAGGAGGTGTTGACTCGCTAATCAAACAATGGCCATCTTGTAAGGTCTATGGCCCCATAGAT
GAACGAATCAGTAATTTAACCCACCCAATTAAACAAGGTCAATCTGTTCAAGTGGGCTCTCTTCACTTTCATATTTTATT
TAATCCAGGGCATACTTCTACCCATATCAGTTATTATGAACCACAACAAGGATGGTTATTTTGTGGAGATACTCTATTTT
CTGCTGGTTGCGGTAGAGTGTTTGATGGAACTATAGAGGAATTGCATGAATCCCTGCTCTTATTTAAAAAACTCCCGCGA
AATACCAAAATTTTTTGTGCTCATGAATACACCTTACAGAATTTAAAATTTGCACATACCGTTGAGCCCTGCAATTCATC
AGTCATCAATTACATGCAACAAATACTGAAACAACCATCACCTTGTACTCTCCCGTCTAATATAGACCTGGAATTATCAA
TTAATCCTTTTTTACGTACTGATAAAGAGCAAGTAAAACAATATGCTCTCTCGCATGGTGCGAATTCTTCAGATTCTCTG
GACGTTTTTGAGGTATTGAGAAATCAAAAAAACTCGTTCAAATAA

Upstream 100 bases:

>100_bases
AATCAAGGCAGTTTTAGAGACATTAACAACTTATTTTAACCCTGGCTCATCTTCTTATATGATTGAATCAGGTTCAAAAT
TATGTCACGGGCTATAAATT

Downstream 100 bases:

>100_bases
TACAACAAAGGATCAAATAAAAATCCATTTGCACCAAATTAAACCAAAAAGGGTTTAATCCTTCATTAATTTTTAGTAAA
CTGATTAGCCAATTATAATA

Product: hydroxyacylglutathione hydrolase (glyoxalase II)

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTILPISAFSDNYIWTFIDKIAGVLDCVDPGESAPIIRFAQSNQLTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPID
ERISNLTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
NTKIFCAHEYTLQNLKFAHTVEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
DVFEVLRNQKNSFK

Sequences:

>Translated_254_residues
MTILPISAFSDNYIWTFIDKIAGVLDCVDPGESAPIIRFAQSNQLTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPID
ERISNLTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
NTKIFCAHEYTLQNLKFAHTVEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
DVFEVLRNQKNSFK
>Mature_253_residues
TILPISAFSDNYIWTFIDKIAGVLDCVDPGESAPIIRFAQSNQLTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPIDE
RISNLTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPRN
TKIFCAHEYTLQNLKFAHTVEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSLD
VFEVLRNQKNSFK

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=37.2623574144487, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=37.2623574144487, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI14150041, Length=262, Percent_Identity=33.587786259542, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI116642887, Length=266, Percent_Identity=37.593984962406, Blast_Score=150, Evalue=9e-37,
Organism=Homo sapiens, GI21703352, Length=266, Percent_Identity=37.593984962406, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI46361987, Length=204, Percent_Identity=35.2941176470588, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI1786406, Length=223, Percent_Identity=41.7040358744395, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1787158, Length=160, Percent_Identity=30.625, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=225, Percent_Identity=35.1111111111111, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320478, Length=263, Percent_Identity=28.1368821292776, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324614, Length=255, Percent_Identity=28.2352941176471, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356335, Length=264, Percent_Identity=39.7727272727273, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24667703, Length=264, Percent_Identity=39.7727272727273, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24667711, Length=264, Percent_Identity=39.7727272727273, Blast_Score=183, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28730; Mature: 28599

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILPISAFSDNYIWTFIDKIAGVLDCVDPGESAPIIRFAQSNQLTLRTILLTHHHYDHI
CEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEEEHHCCCHHC
GGVDSLIKQWPSCKVYGPIDERISNLTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYE
CCHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCEEEEEC
PQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPRNTKIFCAHEYTLQNLKFAHT
CCCCEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHCCEEEEE
VEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCCCCH
DVFEVLRNQKNSFK
HHHHHHHHHHCCCC
>Mature Secondary Structure 
TILPISAFSDNYIWTFIDKIAGVLDCVDPGESAPIIRFAQSNQLTLRTILLTHHHYDHI
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEEEHHCCCHHC
GGVDSLIKQWPSCKVYGPIDERISNLTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYE
CCHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCEEEEEC
PQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPRNTKIFCAHEYTLQNLKFAHT
CCCCEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHCCEEEEE
VEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCCCCH
DVFEVLRNQKNSFK
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA