Click here to switch to the map view.

The map label for this gene is potD [H]

Identifier: 54294081

GI number: 54294081

Start: 1275022

End: 1276044

Strand: Reverse

Name: potD [H]

Synonym: lpl1145

Alternate gene names: 54294081

Gene position: 1276044-1275022 (Counterclockwise)

Preceding gene: 54294082

Following gene: 54294080

Centisome position: 38.14

GC content: 38.81

Gene sequence:

>1023_bases
ATGAAAATTTTTTCATTGCTGCTCGCTCTTCTTACATTTAAGGCGTTCTCCAATCCAGTAGTCAATGTCTATGCCTGGGG
AGGTGAAATCCCTAAAAAAATAATTCATCAATTCGAACAAGAGTCAGGAATTAAAGTCAATTTTTCGACTTATGATAGCA
ATGAAACCATGTATGCCAAGCTAAGAGCCAGTCAAAAGACCATTTACGATGTCATCCTCCCTTCCGCTTATTTTGTAGAG
CGCATGCAAAAACAAGGCATGTTGCAATTACTCGATCATAAACTGCTTCCGAATCTTCATAATCTGGACGAACAATTTGT
TAAAAATGATTATGATCCGGGTAATCGTTATAGTATACCCATCATCTGGGGAGCAACCGGGGTTTTCTTTAATCATAATT
CCGTATCAAACCCGCCCAAAAAATGGCAAGGCTTATGGGATAAAAAGTGGCGTAATCAGCTAATGTTACTTGATGATTCA
CGCGAAATATTTGCCATAGCTCTGATGAGTTTAGGGTTTGATCCCAATGATAGCAATCCAAAGCACATAAAAGCAGCCTA
TGAACACTTATTGCAATTGGTACCTAATATTAAGTTATTTGCCAGTGATAGTATTCAAGCCATCATGATAGATGAGGATG
CTCTTATTGGATCAGCATGGAATGGCGATGCCTTCAAAGCACATACGGAAAACAAAGCCATTGAGTTTATTTATCCTCAA
GATGGTTTTGTGATTTGGGTAGATTGTTTGGCTATCCCTAAAAACCCCCCACACCTGAAAGAGGCGTATCAATTCATTAA
TTTTATTCTGAAACCGGAAACAAGCGCTCAAATTGCACTAAAGGAAGGACATGCGATTACCAATGCCGCAGGAAGAGCAT
TATTGCCATTACCTATAAAAAATAACCCTGTGGTTTATCCGCCAGACGAAATACTAAAGAAGGGTTATTTCCAACGCGAC
ATCGGCGAAGAAACACTAGCGCTTTATAATCAATATTGGGAACAGTTAAAACTTGCTTTTTAG

Upstream 100 bases:

>100_bases
GTGCTGGCGTCACGCCTGAACTCAATGCATTGTGTTCAATTACGTTCGGTTTATCAATGATTCTCGTTATTATCTCTCAT
CGCTTGTCAGGTAAATTGAT

Downstream 100 bases:

>100_bases
TTTTCTGTTGTGAAGTAGCGCTAAGATATCCAGATGAAGTACTTCATTAACTCAACGAATTAAAATTCAGAAAGCAAGAT
TTATTGCCCATATTATATTC

Product: hypothetical protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGIKVNFSTYDSNETMYAKLRASQKTIYDVILPSAYFVE
RMQKQGMLQLLDHKLLPNLHNLDEQFVKNDYDPGNRYSIPIIWGATGVFFNHNSVSNPPKKWQGLWDKKWRNQLMLLDDS
REIFAIALMSLGFDPNDSNPKHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDALIGSAWNGDAFKAHTENKAIEFIYPQ
DGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSAQIALKEGHAITNAAGRALLPLPIKNNPVVYPPDEILKKGYFQRD
IGEETLALYNQYWEQLKLAF

Sequences:

>Translated_340_residues
MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGIKVNFSTYDSNETMYAKLRASQKTIYDVILPSAYFVE
RMQKQGMLQLLDHKLLPNLHNLDEQFVKNDYDPGNRYSIPIIWGATGVFFNHNSVSNPPKKWQGLWDKKWRNQLMLLDDS
REIFAIALMSLGFDPNDSNPKHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDALIGSAWNGDAFKAHTENKAIEFIYPQ
DGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSAQIALKEGHAITNAAGRALLPLPIKNNPVVYPPDEILKKGYFQRD
IGEETLALYNQYWEQLKLAF
>Mature_340_residues
MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGIKVNFSTYDSNETMYAKLRASQKTIYDVILPSAYFVE
RMQKQGMLQLLDHKLLPNLHNLDEQFVKNDYDPGNRYSIPIIWGATGVFFNHNSVSNPPKKWQGLWDKKWRNQLMLLDDS
REIFAIALMSLGFDPNDSNPKHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDALIGSAWNGDAFKAHTENKAIEFIYPQ
DGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSAQIALKEGHAITNAAGRALLPLPIKNNPVVYPPDEILKKGYFQRD
IGEETLALYNQYWEQLKLAF

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=326, Percent_Identity=39.5705521472393, Blast_Score=264, Evalue=7e-72,
Organism=Escherichia coli, GI1787078, Length=338, Percent_Identity=29.8816568047337, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787711, Length=310, Percent_Identity=22.5806451612903, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38901; Mature: 38901

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGIKVNFSTYDSNETMYAK
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEE
LRASQKTIYDVILPSAYFVERMQKQGMLQLLDHKLLPNLHNLDEQFVKNDYDPGNRYSIP
EHHCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEE
IIWGATGVFFNHNSVSNPPKKWQGLWDKKWRNQLMLLDDSREIFAIALMSLGFDPNDSNP
EEEECCEEEEECCCCCCCCHHHCCCCCHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCH
KHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDALIGSAWNGDAFKAHTENKAIEFIYPQ
HHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCEECCCCCCCCEEEECCCCEEEEEECC
DGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSAQIALKEGHAITNAAGRALLPLPIK
CCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEHHCCCCEEEEECCC
NNPVVYPPDEILKKGYFQRDIGEETLALYNQYWEQLKLAF
CCCEEECCHHHHHCCCHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGIKVNFSTYDSNETMYAK
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEE
LRASQKTIYDVILPSAYFVERMQKQGMLQLLDHKLLPNLHNLDEQFVKNDYDPGNRYSIP
EHHCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEE
IIWGATGVFFNHNSVSNPPKKWQGLWDKKWRNQLMLLDDSREIFAIALMSLGFDPNDSNP
EEEECCEEEEECCCCCCCCHHHCCCCCHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCH
KHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDALIGSAWNGDAFKAHTENKAIEFIYPQ
HHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCEECCCCCCCCEEEECCCCEEEEEECC
DGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSAQIALKEGHAITNAAGRALLPLPIK
CCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEHHCCCCEEEEECCC
NNPVVYPPDEILKKGYFQRDIGEETLALYNQYWEQLKLAF
CCCEEECCHHHHHCCCHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]