Click here to switch to the map view.

The map label for this gene is hisF [H]

Identifier: 54293733

GI number: 54293733

Start: 893352

End: 894113

Strand: Direct

Name: hisF [H]

Synonym: lpl0786

Alternate gene names: 54293733

Gene position: 893352-894113 (Clockwise)

Preceding gene: 54293732

Following gene: 54293734

Centisome position: 26.7

GC content: 40.55

Gene sequence:

>762_bases
ATGAGTAATGTCCGTCTAATTGCACGACTAGATATTAAAGGGCCTAACCTAATCAAAGGAGTGCATCTGGAGGGATTAAG
AGTAGTAGGAAATCCCAATGAATATGCTATGGCATATTATGCACAAGGTGCAGATGAATTGATTTATATGGATACAGTTG
CATCTTTGTATGGGCGTAATAATTTGTCAGAGATTGTAAAGACTACTGCTGAAAATGTATTTATCCCAATTACTGTAGGT
GGCGGAATTCGTAGTGTTGATGATGCCAAGCAATTATTGCGTTGCGGTGCCGATAAGGTAGCCATTAATACAGCGGCAAC
TAAAAACCCTGCTCTAATATCTGATATTGCCAGAAGATTTGGGTCTCAATGTGTTGTGTTATCTATCGAAGCAAAACGCA
CTGTGAATGGAAGATGGGAAGTCATGACAGACAATGGACGTGAACATACCGGAATGGATGTTGTTGATTGGGCTAGGAAT
GGCGAAGAATTTGGTGCGGGTGAAATTCTTCTGACCTCTATTGATCAGGAAGGTACGCGTAAGGGATTTGATCTGGAGTT
AGTGAAACAGGTGTCTTCCATAGTTTCTATTCCGGTGATTGCAAGTGGGGGTATGGGTAAGCTGGAAGATTTAACTGAAG
TAGTTTGTGAGTCTAAAGCTGATGCAGTCGCTATGGCCGATGTTTTGCATTATAAAAGAATAGGTTTACCTGAAATCAGA
AAGTATGCTTTAGATAATGGCATTCATGTGAGAGTCTTATGA

Upstream 100 bases:

>100_bases
CTCACTTGTGGAAGAAAGAGGGAGAACAGTGGAAACTTAGACATCAAGTAACAAATCTTGAAAAAACTAAAGCTGAATAT
CTGGATTTGGAGACTGTTTG

Downstream 100 bases:

>100_bases
GTTCAGTTTCAATTTTAGATTATGGGGTAGGCAATTTATTAAGTGTAGCTCGAGCTTTTCAATATTTTGATGCGTCTGTC
AATTTAGTGAGCACACCCGA

Product: hypothetical protein

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVG
GGIRSVDDAKQLLRCGADKVAINTAATKNPALISDIARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARN
GEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSIVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEIR
KYALDNGIHVRVL

Sequences:

>Translated_253_residues
MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVG
GGIRSVDDAKQLLRCGADKVAINTAATKNPALISDIARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARN
GEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSIVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEIR
KYALDNGIHVRVL
>Mature_252_residues
SNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVGG
GIRSVDDAKQLLRCGADKVAINTAATKNPALISDIARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARNG
EEFGAGEILLTSIDQEGTRKGFDLELVKQVSSIVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEIRK
YALDNGIHVRVL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=253, Percent_Identity=35.9683794466403, Blast_Score=170, Evalue=8e-44,
Organism=Escherichia coli, GI87082028, Length=183, Percent_Identity=26.2295081967213, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=279, Percent_Identity=32.9749103942652, Blast_Score=139, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27486; Mature: 27355

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRN
CCCEEEEEEEECCCCCEEEEEEECCEEEECCCCCEEEEEEECCCCCEEHHHHHHHHHCCC
NLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAINTAATKNPALISDIARRF
CHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
GSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARNGEEFGAGEILLTSIDQEGTR
CCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCC
KGFDLELVKQVSSIVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEIR
CCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
KYALDNGIHVRVL
HHHHCCCEEEEEC
>Mature Secondary Structure 
SNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRN
CCEEEEEEEECCCCCEEEEEEECCEEEECCCCCEEEEEEECCCCCEEHHHHHHHHHCCC
NLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAINTAATKNPALISDIARRF
CHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
GSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARNGEEFGAGEILLTSIDQEGTR
CCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCC
KGFDLELVKQVSSIVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEIR
CCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
KYALDNGIHVRVL
HHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11043980 [H]