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The map label for this gene is hisH [H]

Identifier: 54293734

GI number: 54293734

Start: 894110

End: 894751

Strand: Direct

Name: hisH [H]

Synonym: lpl0787

Alternate gene names: 54293734

Gene position: 894110-894751 (Clockwise)

Preceding gene: 54293733

Following gene: 54293735

Centisome position: 26.72

GC content: 38.79

Gene sequence:

>642_bases
ATGAGTTCAGTTTCAATTTTAGATTATGGGGTAGGCAATTTATTAAGTGTAGCTCGAGCTTTTCAATATTTTGATGCGTC
TGTCAATTTAGTGAGCACACCCGAGGAAATTATGAGTGCCGATCGGTTGGTTTTGCCAGGAGTAGGCGCTTTTGAAGATG
GGATGAAAGGATTAACTACATTAAATTTTATAGAACCCATAAAGCAATTTGCACGGAGTGGAAAACCTTTTCTGGGAATA
TGTCTTGGCATGCAGATGATGCTTTCCAAAAGTACTGAATTTGGACAACATGAAGGTCTGGGTCTAATTGCTGGCGAGGT
TGTTAGCGTCCCTTCACATGGAGTTGATGGTCAATTGCATAAAATACCTCATATAGGCTGGAATGAACTGGTTTCCACCT
CGGAAGGCGAGGATTGGTGTCATACTATCCTGAAAAATATACCATTAAATTCTTCAGTATATTTTGTTCATTCTTTTATG
GCCATGCCTAGTAATCCTAAAAAGCGTCTGGCTGATACTTTATATGATGGTCAGGCTATTAGCGCAGTAATAAAAGATGA
AAATATGTACGGATGCCAATTTCATCCTGAAAAAAGCGGGGAAGTAGGATTAAGCATTATTCAACAGTTTTTGCAGATTT
AG

Upstream 100 bases:

>100_bases
CTGATGCAGTCGCTATGGCCGATGTTTTGCATTATAAAAGAATAGGTTTACCTGAAATCAGAAAGTATGCTTTAGATAAT
GGCATTCATGTGAGAGTCTT

Downstream 100 bases:

>100_bases
GGTGAATTAAAAATGAGAATATTGGCAGTAATCCCGGCAAGGGCTGGCTCAAAGAGGCTGCCCGGTAAGAATACCAGATT
ACTTGCCGGAAAACCATTAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1 [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSSVSILDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGI
CLGMQMMLSKSTEFGQHEGLGLIAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI

Sequences:

>Translated_213_residues
MSSVSILDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGI
CLGMQMMLSKSTEFGQHEGLGLIAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI
>Mature_212_residues
SSVSILDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGIC
LGMQMMLSKSTEFGQHEGLGLIAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFMA
MPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=33.6492890995261, Blast_Score=113, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319725, Length=222, Percent_Identity=31.5315315315315, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23238; Mature: 23107

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVSILDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTT
CCCCCHHHHCHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCEECCCCCCHHHHHHHHHH
LNFIEPIKQFARSGKPFLGICLGMQMMLSKSTEFGQHEGLGLIAGEVVSVPSHGVDGQLH
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHEEECCCCCCCCCHH
KIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFMAMPSNPKKRLADTLYDGQAI
CCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHH
SAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI
HHEEECCCEECCEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SSVSILDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTT
CCCCHHHHCHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCEECCCCCCHHHHHHHHHH
LNFIEPIKQFARSGKPFLGICLGMQMMLSKSTEFGQHEGLGLIAGEVVSVPSHGVDGQLH
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHEEECCCCCCCCCHH
KIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFMAMPSNPKKRLADTLYDGQAI
CCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHH
SAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI
HHEEECCCEECCEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA