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The map label for this gene is pleD [H]

Identifier: 54293727

GI number: 54293727

Start: 885911

End: 887461

Strand: Direct

Name: pleD [H]

Synonym: lpl0780

Alternate gene names: 54293727

Gene position: 885911-887461 (Clockwise)

Preceding gene: 54293726

Following gene: 54293728

Centisome position: 26.48

GC content: 34.95

Gene sequence:

>1551_bases
ATGATAGATCAAGAATTAAAGAAAAAAATTGTTAACACAATTAGCCAATATGAGCATAAGTGTGACAAGATGCAAAATCA
AATTAATACACTCAGAAGTGTAATTAATCAATTGATCATTAATCCTATTGGAATCAATTATGAATTGGATGATCGCCTTT
CGCAACTAAGAAATTATTTGGATACGGAGTTTGAACCCGCTGTCATAGAAAAAAAAGTGAAACGTTTGGCAGAAATACTT
TCCAAAATGCAAAGGAAAAAAGCGGAAAATTCTCGTTTAGTCAATGGACTAATCAAACAGGGAGTGGAGTCCATTGGTCG
TATTGCCAATAAATCTCAGGATAAGAGGGCCGTTACAAAATTACAAAAAATGCTGGATACCGAGATGGAAAGCCATGCGA
TTTTGATTTGTTTTAATCAAGTCTTAACTCAATGCATTTCTTCTGTGATTAAGGAGCTTGAGGAGTTACCACAAATTACA
AAAGTCGATACCCCCCAAACAAGTTATCAGTCTGAGATTAGTTTAAAAGTAAATGACAGCTTGCAACAATTACTTAACCA
TTTATCTATCCCTCAGGACTTGGATTCCAAGAGGGGAATCATAAAAACAAATTTGGAACATGCACTCACTGATGAAGAGC
TTAGCACTATTATTGACAATTTGACGGAATTAGTCGTTGAGTCATTTAATGTTGAGCAGAATCGATTCAAAGGGTTTCTT
CAACAGTTAACCGATCAGCTTAATGATTTTGAAATCTATTTAAGAGTATCAGGAGATGCTCGCAAAAAGGCAAGTGAAGA
CAGTCGTTTATTAGAGTGTGGGATTCAGGATAATCTTGATCAAATCAAGAATCATTTAGATACCTCTAAAACGATTGAGG
AATTATCCTCAAAGGTGAGCCAAAATATAAAAATGATTGGTGACAGGTTAAAAGAATATCGACACAATGAAAAAATCCGA
GAGGAAGAATATGCAAAGCAAGTATCTGAGTTGCAAGCCAGATTACAGGAATCCGAGCAAAATGCAGAAGAAATAAAAAA
TTTGCTTGCTTTTCAGAAATACAGAATTAACCACGACAGTTTAACAGGTTTACCTAATAGAGAATCTTATGAAGAGCATA
TTCTGGACTCATTTCAGCGCTGGAAACGAAGTTCAAGCGTATTGTCTCTTGCTGTGTGTGACATCGATCATTTTAAATGT
ATTAATGATAATTTTGGCCATCTTGCAGGAGATAAAGTTCTTAAAAAGGTAGCTATGATTTTTAAATCATCGATCAGGAC
GGTTGATTTTATCGCTCGAATAGGTGGTGAGGAATTTGTGTTTATCTTTGAACAAACCAGCAGCAAGGAAGCTTTTGGAG
TTTTGGAGAAATTACGTAAATTAGTTGAAGATTGTTTGTTTTATTATCGAGACAAAAAAGTCGATGTTACGGTTTCTTTT
GGACTAACTACGGTAACCGAAGAAGATGATATTGAATCTTTATTTATGCGGGCAGACAATGCCATGTATAAAGCTAAAAA
GGCAGGAAAGAACAGGGTAGAAATATTATAG

Upstream 100 bases:

>100_bases
CAAACCAAAAAACCTCCTTTTTGTGATGGATCGCATGCAAAGGTTTTGCTTGAAGTGCTTAAAAACAGGCAAAATAAATG
AACAGGAAAGGAAGTCTTAA

Downstream 100 bases:

>100_bases
TGGAATATATTTCCAACAATTTTTAATTATACTATTTTCGGAGTAAATCCATGGTAACAGATGATTTTCCTCTGATAACG
ATTTTTAAATGAACTTGATC

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MIDQELKKKIVNTISQYEHKCDKMQNQINTLRSVINQLIINPIGINYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEIL
SKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTKLQKMLDTEMESHAILICFNQVLTQCISSVIKELEELPQIT
KVDTPQTSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIR
EEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYEEHILDSFQRWKRSSSVLSLAVCDIDHFKC
INDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGVLEKLRKLVEDCLFYYRDKKVDVTVSF
GLTTVTEEDDIESLFMRADNAMYKAKKAGKNRVEIL

Sequences:

>Translated_516_residues
MIDQELKKKIVNTISQYEHKCDKMQNQINTLRSVINQLIINPIGINYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEIL
SKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTKLQKMLDTEMESHAILICFNQVLTQCISSVIKELEELPQIT
KVDTPQTSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIR
EEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYEEHILDSFQRWKRSSSVLSLAVCDIDHFKC
INDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGVLEKLRKLVEDCLFYYRDKKVDVTVSF
GLTTVTEEDDIESLFMRADNAMYKAKKAGKNRVEIL
>Mature_516_residues
MIDQELKKKIVNTISQYEHKCDKMQNQINTLRSVINQLIINPIGINYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEIL
SKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTKLQKMLDTEMESHAILICFNQVLTQCISSVIKELEELPQIT
KVDTPQTSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIR
EEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYEEHILDSFQRWKRSSSVLSLAVCDIDHFKC
INDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGVLEKLRKLVEDCLFYYRDKKVDVTVSF
GLTTVTEEDDIESLFMRADNAMYKAKKAGKNRVEIL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=35.625, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=36.5714285714286, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=32.7272727272727, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=30.5084745762712, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=36.5853658536585, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=31.6455696202532, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=34.9693251533742, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787802, Length=130, Percent_Identity=37.6923076923077, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787541, Length=179, Percent_Identity=31.2849162011173, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI87081977, Length=207, Percent_Identity=31.8840579710145, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1787056, Length=126, Percent_Identity=37.3015873015873, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788956, Length=248, Percent_Identity=27.4193548387097, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=34.9693251533742, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59581; Mature: 59581

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDQELKKKIVNTISQYEHKCDKMQNQINTLRSVINQLIINPIGINYELDDRLSQLRNYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTEFEPAVIEKKVKRLAEILSKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LQKMLDTEMESHAILICFNQVLTQCISSVIKELEELPQITKVDTPQTSYQSEISLKVNDS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEECHH
LQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVS
HHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHH
QNIKMIGDRLKEYRHNEKIREEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHH
LTGLPNRESYEEHILDSFQRWKRSSSVLSLAVCDIDHFKCINDNFGHLAGDKVLKKVAMI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHH
FKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGVLEKLRKLVEDCLFYYRDKKVDVTVSF
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GLTTVTEEDDIESLFMRADNAMYKAKKAGKNRVEIL
CEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIDQELKKKIVNTISQYEHKCDKMQNQINTLRSVINQLIINPIGINYELDDRLSQLRNYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTEFEPAVIEKKVKRLAEILSKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LQKMLDTEMESHAILICFNQVLTQCISSVIKELEELPQITKVDTPQTSYQSEISLKVNDS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEECHH
LQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVS
HHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHH
QNIKMIGDRLKEYRHNEKIREEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHH
LTGLPNRESYEEHILDSFQRWKRSSSVLSLAVCDIDHFKCINDNFGHLAGDKVLKKVAMI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHH
FKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGVLEKLRKLVEDCLFYYRDKKVDVTVSF
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GLTTVTEEDDIESLFMRADNAMYKAKKAGKNRVEIL
CEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA