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The map label for this gene is lipA [H]

Identifier: 54293728

GI number: 54293728

Start: 887613

End: 888602

Strand: Direct

Name: lipA [H]

Synonym: lpl0781

Alternate gene names: 54293728

Gene position: 887613-888602 (Clockwise)

Preceding gene: 54293727

Following gene: 54293732

Centisome position: 26.53

GC content: 41.82

Gene sequence:

>990_bases
ATGGGCAAATTGATCGATATTCCAATCGTTGTGGAAAGTGGACAAAAATATAAGACATCTCAAGGTGTGACAGCTATTAA
AGATGGAATAAAATCATCAGGTCAAGATCATGAGCGATTGCCAAAACCGAAGTGGCTCCGTATAGTCAATCATACAACTC
CAGCCTATAGCCAAGTTAAAGAGCAAGTGCAAAAACATCGTTTGGCAACAGTTTGTGAAGAAGCGAAATGCCCCAACATC
TCAGAATGTTGGTCTCATGGGACTGCAACTATTATGTTAATGGGAGCAGTATGTACCCGGGCTTGCCGATTTTGTTCTGT
TGATACAGGAAATCCGCATGGTTGGCTTGATGCAGAAGAACCTGAGAATACCGCGGAAACTGTAGCTTTAATGAATCTGG
ATTATGTGGTTTTAACTTCAGTTAACCGTGATGATTTACCTGATGGAGGGGCGAATCATTATGCCAAAACCATTAGAGCA
ATCAAAAAACGATCTCCCCGAACCAAAGTCGAAGCGCTAACTCCCGATTTTCAAGGAAGTGAAAGAGATGTCGCTGTATT
GTTAGATAGTGGCCTTGATGTGTTTGCGCAAAATGTAGAAACGGTTGAGCGTCTGACACACCCGGTAAGAGATAATCGAG
CGGGTTATCAGCAAACCTTGAATGTACTGGCTTTTGCCAAGAAATATCGACCTGATGTTCTGACCAAAACCAGTTTGATG
TTAGGATTGGGTGAAACCGATGAAGAAATTATACGGACTATGGATGACCTCCGAGCTCATCACGTGGATATTTTGACTTT
GGGACAATATTTGCAACCAACTAAAAATCATTTACCTATAGCACGTTACGTAACTCCTGAAACTTTTTCTGAATTAAGGC
AAATTGGTCTGAAAAAAGGGTTTTTTGAAGTCGCTTCTGGCCCACTGGTTCGATCAAGTTATAGGGCTGACCGAGTGTTC
AAACGGGATAATTTAGGGCTTGATGTTTGA

Upstream 100 bases:

>100_bases
TGGTAACAGATGATTTTCCTCTGATAACGATTTTTAAATGAACTTGATCTTATCTTTATGATTTGGGATCATCATAACTC
TTTGTATTTCGGATGGCGTC

Downstream 100 bases:

>100_bases
GATTAGTGAACAGCTTGTGTTTTTCGAATTGGCTATAAATAATTTTATTATTAATTAAAGAAATTAGATTTTTTATTAAA
CCATTAAACCGTTCATGCTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNI
SECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRA
IKKRSPRTKVEALTPDFQGSERDVAVLLDSGLDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLM
LGLGETDEEIIRTMDDLRAHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVF
KRDNLGLDV

Sequences:

>Translated_329_residues
MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNI
SECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRA
IKKRSPRTKVEALTPDFQGSERDVAVLLDSGLDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLM
LGLGETDEEIIRTMDDLRAHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVF
KRDNLGLDV
>Mature_328_residues
GKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNIS
ECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRAI
KKRSPRTKVEALTPDFQGSERDVAVLLDSGLDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLML
GLGETDEEIIRTMDDLRAHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVFK
RDNLGLDV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=53.7414965986395, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI37577164, Length=251, Percent_Identity=54.9800796812749, Blast_Score=272, Evalue=4e-73,
Organism=Escherichia coli, GI1786846, Length=316, Percent_Identity=48.1012658227848, Blast_Score=286, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32564533, Length=295, Percent_Identity=51.1864406779661, Blast_Score=294, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6324770, Length=301, Percent_Identity=51.1627906976744, Blast_Score=305, Evalue=6e-84,
Organism=Drosophila melanogaster, GI221513272, Length=290, Percent_Identity=54.4827586206897, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36754; Mature: 36623

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVK
CCCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCHHEEEECCCCHHHHHHH
EQVQKHRLATVCEEAKCPNISECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEE
HHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRAIKKRSPRTKVEALTPDFQGS
CCCHHHHHHHEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCCCC
ERDVAVLLDSGLDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLM
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
LGLGETDEEIIRTMDDLRAHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKG
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHH
FFEVASGPLVRSSYRADRVFKRDNLGLDV
HHHHHCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVK
CCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCHHEEEECCCCHHHHHHH
EQVQKHRLATVCEEAKCPNISECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEE
HHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRAIKKRSPRTKVEALTPDFQGS
CCCHHHHHHHEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCCCC
ERDVAVLLDSGLDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLM
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
LGLGETDEEIIRTMDDLRAHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKG
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHH
FFEVASGPLVRSSYRADRVFKRDNLGLDV
HHHHHCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA