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The map label for this gene is queF [H]

Identifier: 54293601

GI number: 54293601

Start: 704858

End: 705715

Strand: Direct

Name: queF [H]

Synonym: lpl0654

Alternate gene names: 54293601

Gene position: 704858-705715 (Clockwise)

Preceding gene: 54293599

Following gene: 54293602

Centisome position: 21.07

GC content: 37.06

Gene sequence:

>858_bases
ATGAATAAGGAACTTGAAATTATTTATCAGAATATAGCAAATCAATCGGAGCTGGGACAAAGTTCAAATTATGATGACCA
CTATAATCCTAAAAGGCTGTATCCTATCCCCAGAGCCCCAAAACGCCAGGAAATTAATTTGGATCCCAACTCCACCACTT
TCTATGGTTTTGATTGCTGGAATCATTATGAGGTGTCCTGGTTAAACTCCAAAGGTAAACCTGTTGTTGCGATGGCTGTT
ATTTCTTATGATTGTCATTCCCCGTGTATTATTGAATCCAAGTCACTAAAACTCTATTTTAACTCGTTGAATAACACTAC
TTTCCCTGACGTGGAAACCGTTGTGCAAACTATCAGCAAAGATTTAAGTCACTGCATTGGTTCTGAAGTGGCGGTTAACG
TGTACCCCTTGTCGGAAATCGCTAGTCAAACTATCTATGCTGCATTCGATGGTATTTGTCTTGATAAGCTTGATATTGAA
TGTTCTGTATATCATGTAATGCCTGATTTTCTTTCGACCTCGTCTGAATTAGTAGAAGAGGTATTGTATTCTGATTTACT
TAAATCAAACTGTCTGGTTACCAACCAACCGGATTGGGGAAGCGTTCAAATTATTTATAAAGGTAAAAAGATAAATCATG
AAGGGTTGCTTAAATACCTGATTTCATTTAGAAATCATAATGAATTTCATGAGCAGTGCATTGAACGGATATTTGCAGAC
ATTATGCGATTTTGCCAACCTGAATCATTGACTGTTTATGGACGATATACAAGAAGGGGAGGTCTGGATATTAATCCAAT
CAGGTCAACAGAACCTTGTGTTTTTGATGGGCAAAATATTCGTTTAATACGCCAATAA

Upstream 100 bases:

>100_bases
AAATATAGGCTATTTCCTTTTTTTTTAAGAAAAAATTGACCCTTAGGTAACATGATAGATTATAATCACTTTGTTTTTAG
AGTTACAAAAGGCATCCATA

Downstream 100 bases:

>100_bases
AGGAGGGTACTTATCCCCTCCAGCAGGATAAGGAATAAGTTTTAATCCCGCCTGGGAATTTAATAATTAATTTTCAATAC
ATTACATCTTTCCTATGCTA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNKELEIIYQNIANQSELGQSSNYDDHYNPKRLYPIPRAPKRQEINLDPNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAV
ISYDCHSPCIIESKSLKLYFNSLNNTTFPDVETVVQTISKDLSHCIGSEVAVNVYPLSEIASQTIYAAFDGICLDKLDIE
CSVYHVMPDFLSTSSELVEEVLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLKYLISFRNHNEFHEQCIERIFAD
IMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCVFDGQNIRLIRQ

Sequences:

>Translated_285_residues
MNKELEIIYQNIANQSELGQSSNYDDHYNPKRLYPIPRAPKRQEINLDPNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAV
ISYDCHSPCIIESKSLKLYFNSLNNTTFPDVETVVQTISKDLSHCIGSEVAVNVYPLSEIASQTIYAAFDGICLDKLDIE
CSVYHVMPDFLSTSSELVEEVLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLKYLISFRNHNEFHEQCIERIFAD
IMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCVFDGQNIRLIRQ
>Mature_285_residues
MNKELEIIYQNIANQSELGQSSNYDDHYNPKRLYPIPRAPKRQEINLDPNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAV
ISYDCHSPCIIESKSLKLYFNSLNNTTFPDVETVVQTISKDLSHCIGSEVAVNVYPLSEIASQTIYAAFDGICLDKLDIE
CSVYHVMPDFLSTSSELVEEVLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLKYLISFRNHNEFHEQCIERIFAD
IMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCVFDGQNIRLIRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=271, Percent_Identity=50.1845018450184, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 32733; Mature: 32733

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELEIIYQNIANQSELGQSSNYDDHYNPKRLYPIPRAPKRQEINLDPNSTTFYGFDCW
CCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEECCCCCCCCCEEECCCCCCEEEEEECC
NHYEVSWLNSKGKPVVAMAVISYDCHSPCIIESKSLKLYFNSLNNTTFPDVETVVQTISK
CCEEEEEECCCCCCEEEEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHH
DLSHCIGSEVAVNVYPLSEIASQTIYAAFDGICLDKLDIECSVYHVMPDFLSTSSELVEE
HHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCEEEEEECCEEEEEEECHHHHCCHHHHHHH
VLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLKYLISFRNHNEFHEQCIERIFAD
HHHHHHHHCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCVFDGQNIRLIRQ
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEEC
>Mature Secondary Structure
MNKELEIIYQNIANQSELGQSSNYDDHYNPKRLYPIPRAPKRQEINLDPNSTTFYGFDCW
CCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEECCCCCCCCCEEECCCCCCEEEEEECC
NHYEVSWLNSKGKPVVAMAVISYDCHSPCIIESKSLKLYFNSLNNTTFPDVETVVQTISK
CCEEEEEECCCCCCEEEEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHH
DLSHCIGSEVAVNVYPLSEIASQTIYAAFDGICLDKLDIECSVYHVMPDFLSTSSELVEE
HHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCEEEEEECCEEEEEEECHHHHCCHHHHHHH
VLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLKYLISFRNHNEFHEQCIERIFAD
HHHHHHHHCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCVFDGQNIRLIRQ
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA