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The map label for this gene is ecfE [C]

Identifier: 54293495

GI number: 54293495

Start: 591995

End: 593422

Strand: Direct

Name: ecfE [C]

Synonym: lpl0544

Alternate gene names: 54293495

Gene position: 591995-593422 (Clockwise)

Preceding gene: 54293494

Following gene: 54293496

Centisome position: 17.69

GC content: 39.71

Gene sequence:

>1428_bases
ATGGTTTTACTTTGGATTAACCTACATACCACTGGGAATTTAATTTTCTGGTCGTATCAATTTTTGGATAACGATATGCT
TTCAACGTTGCTGTACTTTTTATTAGCATTGGTATTGTTAGTCACCATACATGAATACGGCCACTTTCAAGTAGCGCGAT
GGTGTGGTGTGAAAGTGTTAAGGTTTTCATTTGGCTTTGGAAAGATTCTTGCGCGTTTTTATGATAAGAAAGGAACAGAG
TATGCCTGGTCTTTGTTTCCTTTGGGTGGCTATGTCAAAATGCTTGATGAAACAGAAGGAGAAGTTTCTGAAAAAGAAAA
GCCTTTCGCTTTCAATAATCAATCTGTTCTTGTCAGGATAGCTATTGTAGTAGCAGGGCCTTTATTCAATTTTATTTTTG
CGTTTGTAGCATTGTGGTTAGTTTTGGTTATCGGCATGCACTCTTTGGCTCCGATGATTGAGTCAGTAAAACCAAATAGT
ATTGCTGCACGAGCTGGCCTAGTCCCCAAGCAGGAAATCCTTGCTCTTAATGGAGTTAAAATTAATAGTTGGCGAGATTT
TCAATATGAAATAATGCCTCTTATCGGTTCCCAGGAAACAGTGAGCTTGACTGTAAAATCAATGATAAATGGTGAAGAAA
GCACTTTGTATTTGCCTTTGGATAAGTGGCAGTTGGATGATAAAAAGCCTGATCCTATTAAAAGCCTGGGCATCATTCCT
TTTATACCGACGATTCCACCCATAGTTGGTGAAGTGGTTCCCGATTCTCCCGCTGAAAAAGCAGGCTTAAAAATAGGGGA
TGAAATTATCAGTGTCAATGGTCAGCATTTCAATGATTGGTTGTATTTAGTCAGCTATGTAAGGGAAAGGCCCAATTCTC
AAATCAATTTGGATATTAAACGCCAAGGTAAATTGCTGAATATAACGGTACACACTGGAAGTCAGGATAATAATGGAAAA
TTAGAGGGGTTAATAGGAGTTAGATCTCAGAAAGTAGATTGGCCTGCTCATTGGCTACGGCTGGAGCAACAGCCACCAAT
TTCAGCACTTGGTACGGCTTTCAAGCAAACAGTACAACTAACGGGCACAACTTTTATCTTAATGGGAAGATTAGTGACTG
GAAAATTGGGATTAAACAGTATTAGCGGTCCCGTCGGCATAGCCCAGGGGGCCGGCGACTCCGGCCGCGGAGGATTGGTA
TCCTATTTATTTTTTCTGGCTTTAGTAAGCATTAGCCTGGGAGCGCTAAATTTATTACCAATCCCTATGTTGGATGGCGG
TCATCTTTTGTATTATGTCTTGGAAATCATAAGAAGAAAGCCATTATCGGACGGAGTTAAATCTGTAGGGATCTATTTTG
GATTATTACTGTTGGTTGCTTTAATGTTTGTTGCTCTTAGTAATGATATATCAAGATTAACCAGCTAG

Upstream 100 bases:

>100_bases
GTATATTAAAACGGCGTTGTCAATTAAAAGATACTGGAAATATTATTCCAGGTCATGGCGGTATATTAGATCGACTGGAT
AGTCTAATTGCCGCGTTGCC

Downstream 100 bases:

>100_bases
GATTTGGCGAGAGTAATTCTAATTTCTGTATGATTAATGAATGAAATGATGTTTTAGCGACAAAGAAACTTGACAGTAGT
TTCTAGTTCCTATAAAAGGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MVLLWINLHTTGNLIFWSYQFLDNDMLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKILARFYDKKGTE
YAWSLFPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRIAIVVAGPLFNFIFAFVALWLVLVIGMHSLAPMIESVKPNS
IAARAGLVPKQEILALNGVKINSWRDFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIIP
FIPTIPPIVGEVVPDSPAEKAGLKIGDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKRQGKLLNITVHTGSQDNNGK
LEGLIGVRSQKVDWPAHWLRLEQQPPISALGTAFKQTVQLTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLV
SYLFFLALVSISLGALNLLPIPMLDGGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS

Sequences:

>Translated_475_residues
MVLLWINLHTTGNLIFWSYQFLDNDMLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKILARFYDKKGTE
YAWSLFPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRIAIVVAGPLFNFIFAFVALWLVLVIGMHSLAPMIESVKPNS
IAARAGLVPKQEILALNGVKINSWRDFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIIP
FIPTIPPIVGEVVPDSPAEKAGLKIGDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKRQGKLLNITVHTGSQDNNGK
LEGLIGVRSQKVDWPAHWLRLEQQPPISALGTAFKQTVQLTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLV
SYLFFLALVSISLGALNLLPIPMLDGGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS
>Mature_475_residues
MVLLWINLHTTGNLIFWSYQFLDNDMLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKILARFYDKKGTE
YAWSLFPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRIAIVVAGPLFNFIFAFVALWLVLVIGMHSLAPMIESVKPNS
IAARAGLVPKQEILALNGVKINSWRDFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIIP
FIPTIPPIVGEVVPDSPAEKAGLKIGDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKRQGKLLNITVHTGSQDNNGK
LEGLIGVRSQKVDWPAHWLRLEQQPPISALGTAFKQTVQLTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLV
SYLFFLALVSISLGALNLLPIPMLDGGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS

Specific function: Unknown

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=444, Percent_Identity=43.9189189189189, Blast_Score=353, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 52585; Mature: 52585

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50106 PDZ ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLWINLHTTGNLIFWSYQFLDNDMLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVL
CEEEEEEEECCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCHHHE
RFSFGFGKILARFYDKKGTEYAWSLFPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRI
EHHCCHHHHHHHHHCCCCCCEEEEEECCCCHHEEHHCCCCCCCCCCCCEEECCCCEEEEE
AIVVAGPLFNFIFAFVALWLVLVIGMHSLAPMIESVKPNSIAARAGLVPKQEILALNGVK
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHEEECCEE
INSWRDFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIIP
ECCCCCCEEEEEEECCCCCEEEEEHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCC
FIPTIPPIVGEVVPDSPAEKAGLKIGDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIK
CCCCCCHHHHHCCCCCCCHHCCCEECCHHEEECCCCHHHHHHHHHHHHHCCCCEEEEEEE
RQGKLLNITVHTGSQDNNGKLEGLIGVRSQKVDWPAHWLRLEQQPPISALGTAFKQTVQL
CCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHEECCCCCHHHHHHHHHHHHHH
TGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLVSYLFFLALVSISLGALNLLP
CCCCEEHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEE
IPMLDGGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVLLWINLHTTGNLIFWSYQFLDNDMLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVL
CEEEEEEEECCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCHHHE
RFSFGFGKILARFYDKKGTEYAWSLFPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRI
EHHCCHHHHHHHHHCCCCCCEEEEEECCCCHHEEHHCCCCCCCCCCCCEEECCCCEEEEE
AIVVAGPLFNFIFAFVALWLVLVIGMHSLAPMIESVKPNSIAARAGLVPKQEILALNGVK
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHEEECCEE
INSWRDFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIIP
ECCCCCCEEEEEEECCCCCEEEEEHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCC
FIPTIPPIVGEVVPDSPAEKAGLKIGDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIK
CCCCCCHHHHHCCCCCCCHHCCCEECCHHEEECCCCHHHHHHHHHHHHHCCCCEEEEEEE
RQGKLLNITVHTGSQDNNGKLEGLIGVRSQKVDWPAHWLRLEQQPPISALGTAFKQTVQL
CCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHEECCCCCHHHHHHHHHHHHHH
TGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLVSYLFFLALVSISLGALNLLP
CCCCEEHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEE
IPMLDGGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]