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The map label for this gene is artJ [H]

Identifier: 54293480

GI number: 54293480

Start: 575807

End: 576541

Strand: Direct

Name: artJ [H]

Synonym: lpl0529

Alternate gene names: 54293480

Gene position: 575807-576541 (Clockwise)

Preceding gene: 54293478

Following gene: 54293481

Centisome position: 17.21

GC content: 36.19

Gene sequence:

>735_bases
ATGAAAAAGATTTGTCTTTTGTTACTAATCAGTTCTTTTATACTTATATCCAGTTGTAGTGAGCAAAAAAATCAAAATAC
ACTGCGTTTTTCCACTTCAGCAGAATATCCACCTTTTGAATATATTGAAAATGGAGAAATAAAAGGATTTGATATTGATT
TGGCAAAATTGATTGCCAAAGAGTTGGGTAAAGAAGCCGTTTTTGACAACATGCAGTTTAGTACGGTTTTGCCTGCATTG
AATTCAGGCCAGGATGACGTTGCGATTGCCACAATTACTATTACCGAGGAACGTAAGAAAAATTTTGATTTTTCCATACC
TTATTATTTTGAAGGAATGGCTGCCGTTTTTGATATTTCACAACCAGTGAAAGAGATCTCGCAATTAACAGGAAAAAAAA
TAGCAGTCCAGCTGGGTAGTGTTATGGAAATTTGGCTTCATCAAAATTTTCCTAATGCTGAAATTACGGCTTTGGATAAT
AACAACCAAGCGATAGAAGCTTTAATCGCAGGTCATGTTGATGTGGTCCTTATGGATGGTGCACAAGGGGGTGTTTATAG
CAAGAAATATCCAGGACTGTCTTATTCCATCCTGGCGAAAGCGAATGAAGGTTATGGATTGGTCTTAAGAAAAGATTCTC
CTCTAAGGGATGAAATCAATTCCGCATTGGAAAATCTTAAAGCGAAGGGTGAAATTCAAAAATTGGAATCCATTTGGATA
AAGGGGTCAATATGA

Upstream 100 bases:

>100_bases
AATATAAATCACTAAAATTGAAATTATATCCATTTTTTCTTGACAGTACAAATCCTGATCATGTATATTCACAAAACGAA
TAAATATCGGATACATATTC

Downstream 100 bases:

>100_bases
CGGATGTGATGCAAAATCTGTTTTTCATTGGCAAAGGAATAGTTCTGACTATGCAATTATTAACAGGGGGTATGCTGATT
GGAGTATTGCTTGGAACTCT

Product: hypothetical protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKKICLLLLISSFILISSCSEQKNQNTLRFSTSAEYPPFEYIENGEIKGFDIDLAKLIAKELGKEAVFDNMQFSTVLPAL
NSGQDDVAIATITITEERKKNFDFSIPYYFEGMAAVFDISQPVKEISQLTGKKIAVQLGSVMEIWLHQNFPNAEITALDN
NNQAIEALIAGHVDVVLMDGAQGGVYSKKYPGLSYSILAKANEGYGLVLRKDSPLRDEINSALENLKAKGEIQKLESIWI
KGSI

Sequences:

>Translated_244_residues
MKKICLLLLISSFILISSCSEQKNQNTLRFSTSAEYPPFEYIENGEIKGFDIDLAKLIAKELGKEAVFDNMQFSTVLPAL
NSGQDDVAIATITITEERKKNFDFSIPYYFEGMAAVFDISQPVKEISQLTGKKIAVQLGSVMEIWLHQNFPNAEITALDN
NNQAIEALIAGHVDVVLMDGAQGGVYSKKYPGLSYSILAKANEGYGLVLRKDSPLRDEINSALENLKAKGEIQKLESIWI
KGSI
>Mature_244_residues
MKKICLLLLISSFILISSCSEQKNQNTLRFSTSAEYPPFEYIENGEIKGFDIDLAKLIAKELGKEAVFDNMQFSTVLPAL
NSGQDDVAIATITITEERKKNFDFSIPYYFEGMAAVFDISQPVKEISQLTGKKIAVQLGSVMEIWLHQNFPNAEITALDN
NNQAIEALIAGHVDVVLMDGAQGGVYSKKYPGLSYSILAKANEGYGLVLRKDSPLRDEINSALENLKAKGEIQKLESIWI
KGSI

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=247, Percent_Identity=33.6032388663968, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=31.8725099601594, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1788228, Length=240, Percent_Identity=30, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787031, Length=242, Percent_Identity=30.9917355371901, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788648, Length=224, Percent_Identity=29.0178571428571, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1788649, Length=249, Percent_Identity=25.7028112449799, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26990; Mature: 26990

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKICLLLLISSFILISSCSEQKNQNTLRFSTSAEYPPFEYIENGEIKGFDIDLAKLIAK
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCCEEEECHHHHHHHHH
ELGKEAVFDNMQFSTVLPALNSGQDDVAIATITITEERKKNFDFSIPYYFEGMAAVFDIS
HHCHHHHHCCCCHHHHHCCCCCCCCCEEEEEEEEEHHHHCCCCCCCCHHHCCHHHHHHHH
QPVKEISQLTGKKIAVQLGSVMEIWLHQNFPNAEITALDNNNQAIEALIAGHVDVVLMDG
HHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCEEEEEEEC
AQGGVYSKKYPGLSYSILAKANEGYGLVLRKDSPLRDEINSALENLKAKGEIQKLESIWI
CCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHEEE
KGSI
ECCC
>Mature Secondary Structure
MKKICLLLLISSFILISSCSEQKNQNTLRFSTSAEYPPFEYIENGEIKGFDIDLAKLIAK
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCCEEEECHHHHHHHHH
ELGKEAVFDNMQFSTVLPALNSGQDDVAIATITITEERKKNFDFSIPYYFEGMAAVFDIS
HHCHHHHHCCCCHHHHHCCCCCCCCCEEEEEEEEEHHHHCCCCCCCCHHHCCHHHHHHHH
QPVKEISQLTGKKIAVQLGSVMEIWLHQNFPNAEITALDNNNQAIEALIAGHVDVVLMDG
HHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCEEEEEEEC
AQGGVYSKKYPGLSYSILAKANEGYGLVLRKDSPLRDEINSALENLKAKGEIQKLESIWI
CCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHEEE
KGSI
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]