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The map label for this gene is araD

Identifier: 54020507

GI number: 54020507

Start: 518808

End: 519530

Strand: Reverse

Name: araD

Synonym: mhp439

Alternate gene names: 54020507

Gene position: 519530-518808 (Counterclockwise)

Preceding gene: 54020508

Following gene: 54020506

Centisome position: 58.19

GC content: 37.07

Gene sequence:

>723_bases
ATGAAAATTAAAGACCGTAACGAACTTGAAGAACTCCAAAAAGCCGTTCTAGAAGCAAATCTTTTACTTTATAAGTCCAA
ATTAGCACTTCATACCTGGGGAAATGTCTCAGCAATTAGCAAAGATCGCAGTTATTATGTTATCAAACCCAGCGGAATTC
CTTATGAAAAAATGAAGTTTTCAGATATGGTTCCTGTCGATCTTGAAAATAATGTCCTTGAAACTAATCTAAACCCTTCA
AGTGATACTCCCACGCATTCACTGATCTACAAAGCCGATTCACGAATTCAAGCCATCGTCCATACCCATTCGCCTTTTGC
AGTTGCTTGAGCTCAAGCCGGAAAAGACATTCCAGCTTTAGGCACAACCCATGCAGATAACTTCTATGGTTCAATTCCTT
GTACAAATTCTTTATCTGATCAACAAATTCAAGGCGATTATGAACATAATACCGGGCTTGTTATCCTCGATCACTTTCAA
AAAAACAAGCTTGATTATATCGGAACTCCGGCTGTTTTAGTCAAAGAACATGGTCCTTTTTGTTGGTCAAATAAATCTGC
TGAGGAGGCAGTTAAACTTGCTATGACTTTTGAAGAAGTAGCCAAAATGGCTTTTTATACAAAAGTGATTAATCCTTACC
AAAACCAAGCAAATCCTATTCTCCAAAAAAAACATTATGAGCGCAAACACGGAAAAAATGCCTATTATGGACAAAAAAAG
TAG

Upstream 100 bases:

>100_bases
AATTTTGACCAAAATGCCGAATTTGAGCAAAATTTTCAAAAACAGGTTGGGATAATTCGTTCAGCCCGTGAATTTTTTCA
AGAAAAGTTGAGGTTAGCCA

Downstream 100 bases:

>100_bases
TCTAATTATCCCCAAAGATCTTTATAAACTAGCTCAGATTGATAATATCGTCTTTGATCTTGATGGCACCTTACTAACTT
CAGATCATGAAATTAGCCTT

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKIKDRNELEELQKAVLEANLLLYKSKLALHTWGNVSAISKDRSYYVIKPSGIPYEKMKFSDMVPVDLENNVLETNLNPS
SDTPTHSLIYKADSRIQAIVHTHSPFAVAWAQAGKDIPALGTTHADNFYGSIPCTNSLSDQQIQGDYEHNTGLVILDHFQ
KNKLDYIGTPAVLVKEHGPFCWSNKSAEEAVKLAMTFEEVAKMAFYTKVINPYQNQANPILQKKHYERKHGKNAYYGQKK

Sequences:

>Translated_240_residues
MKIKDRNELEELQKAVLEANLLLYKSKLALHTWGNVSAISKDRSYYVIKPSGIPYEKMKFSDMVPVDLENNVLETNLNPS
SDTPTHSLIYKADSRIQAIVHTHSPFAVA*AQAGKDIPALGTTHADNFYGSIPCTNSLSDQQIQGDYEHNTGLVILDHFQ
KNKLDYIGTPAVLVKEHGPFCWSNKSAEEAVKLAMTFEEVAKMAFYTKVINPYQNQANPILQKKHYERKHGKNAYYGQKK
>Mature_240_residues
MKIKDRNELEELQKAVLEANLLLYKSKLALHTWGNVSAISKDRSYYVIKPSGIPYEKMKFSDMVPVDLENNVLETNLNPS
SDTPTHSLIYKADSRIQAIVHTHSPFAVA*AQAGKDIPALGTTHADNFYGSIPCTNSLSDQQIQGDYEHNTGLVILDHFQ
KNKLDYIGTPAVLVKEHGPFCWSNKSAEEAVKLAMTFEEVAKMAFYTKVINPYQNQANPILQKKHYERKHGKNAYYGQKK

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786247, Length=231, Percent_Identity=51.9480519480519, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1790642, Length=231, Percent_Identity=51.0822510822511, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1790008, Length=231, Percent_Identity=48.0519480519481, Blast_Score=212, Evalue=2e-56,

Paralogues:

None

Copy number: 136 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 26939; Mature: 26939

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKDRNELEELQKAVLEANLLLYKSKLALHTWGNVSAISKDRSYYVIKPSGIPYEKMKF
CCCCCHHHHHHHHHHHHHHHHHEEECEEEEEECCCEEEEECCCEEEEECCCCCCHHHHHC
SDMVPVDLENNVLETNLNPSSDTPTHSLIYKADSRIQAIVHTHSPFAVAAQAGKDIPALG
CCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEHHCCCCCCCCC
TTHADNFYGSIPCTNSLSDQQIQGDYEHNTGLVILDHFQKNKLDYIGTPAVLVKEHGPFC
CCCCCCCEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCC
WSNKSAEEAVKLAMTFEEVAKMAFYTKVINPYQNQANPILQKKHYERKHGKNAYYGQKK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIKDRNELEELQKAVLEANLLLYKSKLALHTWGNVSAISKDRSYYVIKPSGIPYEKMKF
CCCCCHHHHHHHHHHHHHHHHHEEECEEEEEECCCEEEEECCCEEEEECCCCCCHHHHHC
SDMVPVDLENNVLETNLNPSSDTPTHSLIYKADSRIQAIVHTHSPFAVAAQAGKDIPALG
CCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEHHCCCCCCCCC
TTHADNFYGSIPCTNSLSDQQIQGDYEHNTGLVILDHFQKNKLDYIGTPAVLVKEHGPFC
CCCCCCCEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCC
WSNKSAEEAVKLAMTFEEVAKMAFYTKVINPYQNQANPILQKKHYERKHGKNAYYGQKK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]