Click here to switch to the map view.

The map label for this gene is glpK

Identifier: 54020480

GI number: 54020480

Start: 438935

End: 440467

Strand: Reverse

Name: glpK

Synonym: mhp370

Alternate gene names: 54020480

Gene position: 440467-438935 (Counterclockwise)

Preceding gene: 54020481

Following gene: 54020479

Centisome position: 49.34

GC content: 34.25

Gene sequence:

>1533_bases
ATGCTAGAAAATACAGCTAAAAAATTTATTATCACCCTCGATTCAGGGACAACTTCATGTCGAAGTTTGATTTTTGATCA
AAAAGGTAGGGTTATTTCACTTGTCCAAAAAGAATTTAGTCAACATTTTCCCAGATCAGGATGAGTCGAACATGACCCGA
TTGAAATATGGAATACCCAGGTCTATACAATGCAGGCAGCAAAAACAAAAGCGGGGTTAAAATCGACTGATTTTGTCGCC
CTTGGACTAACAAATCAACGGGAAACTGTCGTTTTATGAGATAAAACAAATGGCGAGCCAGTCTATAATGCAATTGTCTG
GCAGGATCGCCGCACGAGTGATTTTTGTGATTATTTAATTGAAAAAGGTTATCAAAATTATATAAAAGAAGTAACAGGTC
TAATTATTAATCCTTATTTTAGCGGGACAAAAATTCGCTGAATTTTAAAAAATGTTAAAAAAGCAAAGGAAGTTTTAGCT
GCTGGTAATCTTTTAGCAGGAACAATTGATAGCTGACTTCTTTGAAAATTAACTAACGGAAAAGTACATGCAACTGATAT
AACAAACGCTTCAAGAACGATGTTATTTGATATAAAAAAACGAAAATGAGACCAAAAAATTCTTGATCTTTTAGAAATTC
CTTACAGTATTCTCCCAAAAGTTTTAGCATCAGCAGATGATTATGGATTTGTTTCTCCTTTTCATTGATCAATTAATGCA
AAAACAAAAGTACCAATTTATGCACTAATTGGCGATCAACAATCGGCCTTATTTGGTCAACTTGCAACTGAATTTGGAAT
GGTTAAAAATACCTATGGAACCGGATGTTTTATGCTAGTTAATACTGGTGAAAAACTAATAAAATCAAAGAATAATTTAC
TTACAACAGTGGCCTGACAAATCGGAAATAATCCAATCCAGTACGCACTTGAAGGATCAGTTTTTATTGCCGGAGCCGCA
ATCCAGTGGCTCAGAGATGGACTTTCTTTGATTGAAAACTCTTCAGATTGCGATTCTTTAGTCTCAAAAGCTAAAAAAAA
TGATTATCATATTATTCTTGTTCCATCTTTTACCGGACTTGGGGCACCTTATTGGGACTCCTATTCGCGTGGGGCAATTT
TTGGACTTGAAAGGGGAACAAAAAAAGAACATCTTATCAAAGCAAGTCTTGAATCAATTGCATTTCAGGCAAATGATCTA
ATTAAGGCGATCGAATCAGATCTAGGAACAAAAATCAGCTTGATGAAAGTTGATGGTGGAGTTTCTAAAAGTGATTATCT
AATGCAATTTCAGTCATCAATTTCGCAGGTTGAAATCCAAAGGCCAAAAAATATTGAAACAACAGCAATGGGAGCAGCTT
TTCTTGCGGGTTTATATGCCAAATTTTGGAATTCACTAGCTGAATTAAAGCAAATTTTAGTTCCTGAAAAAGTTTTTGTC
CCTGAATTTGACCAATTTATGGTAAAAAAATTAACCTCCAATTGGGACTTAGCTGTTAGAAAAACGCTAAATTGGAAAAA
AGATATTAAATAA

Upstream 100 bases:

>100_bases
AAAAATAATTAAAAAATTAACAAAATTGTTAATTTCACAGGGCTACTTTGGATTAAAATGTTATAATTCAAATATATAAA
AATTGAAAGGATTTAAAAAA

Downstream 100 bases:

>100_bases
AAAGGAATCCAAGATGAATAATCTCTTTTTTTTATCAGAATTTCTTGGGACTGCGATACTAATTTTACTCGGAAATGGCG
TAAATTATAGTGTAAATGCG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MLENTAKKFIITLDSGTTSCRSLIFDQKGRVISLVQKEFSQHFPRSGWVEHDPIEIWNTQVYTMQAAKTKAGLKSTDFVA
LGLTNQRETVVLWDKTNGEPVYNAIVWQDRRTSDFCDYLIEKGYQNYIKEVTGLIINPYFSGTKIRWILKNVKKAKEVLA
AGNLLAGTIDSWLLWKLTNGKVHATDITNASRTMLFDIKKRKWDQKILDLLEIPYSILPKVLASADDYGFVSPFHWSINA
KTKVPIYALIGDQQSALFGQLATEFGMVKNTYGTGCFMLVNTGEKLIKSKNNLLTTVAWQIGNNPIQYALEGSVFIAGAA
IQWLRDGLSLIENSSDCDSLVSKAKKNDYHIILVPSFTGLGAPYWDSYSRGAIFGLERGTKKEHLIKASLESIAFQANDL
IKAIESDLGTKISLMKVDGGVSKSDYLMQFQSSISQVEIQRPKNIETTAMGAAFLAGLYAKFWNSLAELKQILVPEKVFV
PEFDQFMVKKLTSNWDLAVRKTLNWKKDIK

Sequences:

>Translated_510_residues
MLENTAKKFIITLDSGTTSCRSLIFDQKGRVISLVQKEFSQHFPRSG*VEHDPIEIWNTQVYTMQAAKTKAGLKSTDFVA
LGLTNQRETVVL*DKTNGEPVYNAIVWQDRRTSDFCDYLIEKGYQNYIKEVTGLIINPYFSGTKIR*ILKNVKKAKEVLA
AGNLLAGTIDS*LL*KLTNGKVHATDITNASRTMLFDIKKRK*DQKILDLLEIPYSILPKVLASADDYGFVSPFH*SINA
KTKVPIYALIGDQQSALFGQLATEFGMVKNTYGTGCFMLVNTGEKLIKSKNNLLTTVA*QIGNNPIQYALEGSVFIAGAA
IQWLRDGLSLIENSSDCDSLVSKAKKNDYHIILVPSFTGLGAPYWDSYSRGAIFGLERGTKKEHLIKASLESIAFQANDL
IKAIESDLGTKISLMKVDGGVSKSDYLMQFQSSISQVEIQRPKNIETTAMGAAFLAGLYAKFWNSLAELKQILVPEKVFV
PEFDQFMVKKLTSNWDLAVRKTLNWKKDIK
>Mature_510_residues
MLENTAKKFIITLDSGTTSCRSLIFDQKGRVISLVQKEFSQHFPRSG*VEHDPIEIWNTQVYTMQAAKTKAGLKSTDFVA
LGLTNQRETVVL*DKTNGEPVYNAIVWQDRRTSDFCDYLIEKGYQNYIKEVTGLIINPYFSGTKIR*ILKNVKKAKEVLA
AGNLLAGTIDS*LL*KLTNGKVHATDITNASRTMLFDIKKRK*DQKILDLLEIPYSILPKVLASADDYGFVSPFH*SINA
KTKVPIYALIGDQQSALFGQLATEFGMVKNTYGTGCFMLVNTGEKLIKSKNNLLTTVA*QIGNNPIQYALEGSVFIAGAA
IQWLRDGLSLIENSSDCDSLVSKAKKNDYHIILVPSFTGLGAPYWDSYSRGAIFGLERGTKKEHLIKASLESIAFQANDL
IKAIESDLGTKISLMKVDGGVSKSDYLMQFQSSISQVEIQRPKNIETTAMGAAFLAGLYAKFWNSLAELKQILVPEKVFV
PEFDQFMVKKLTSNWDLAVRKTLNWKKDIK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=41.304347826087, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI189409120, Length=504, Percent_Identity=41.0714285714286, Blast_Score=339, Evalue=4e-93,
Organism=Homo sapiens, GI4504007, Length=504, Percent_Identity=41.0714285714286, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=40.6374501992032, Blast_Score=333, Evalue=3e-91,
Organism=Homo sapiens, GI88196792, Length=512, Percent_Identity=32.2265625, Blast_Score=244, Evalue=2e-64,
Organism=Escherichia coli, GI1790361, Length=503, Percent_Identity=48.7077534791253, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1789987, Length=476, Percent_Identity=22.2689075630252, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535599, Length=510, Percent_Identity=38.4313725490196, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI71986140, Length=527, Percent_Identity=22.9601518026565, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71986147, Length=262, Percent_Identity=26.7175572519084, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321755, Length=527, Percent_Identity=32.0683111954459, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI17864214, Length=513, Percent_Identity=37.6218323586745, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24654573, Length=446, Percent_Identity=38.5650224215247, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24655555, Length=485, Percent_Identity=34.2268041237113, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24655560, Length=485, Percent_Identity=34.2268041237113, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24655570, Length=485, Percent_Identity=34.2268041237113, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24655574, Length=485, Percent_Identity=34.2268041237113, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24655565, Length=485, Percent_Identity=34.2268041237113, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI19922058, Length=532, Percent_Identity=30.2631578947368, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24652871, Length=501, Percent_Identity=30.3393213572854, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI21357867, Length=526, Percent_Identity=27.9467680608365, Blast_Score=192, Evalue=6e-49,
Organism=Drosophila melanogaster, GI24656367, Length=512, Percent_Identity=28.3203125, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24656372, Length=370, Percent_Identity=29.4594594594595, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45552909, Length=280, Percent_Identity=29.2857142857143, Blast_Score=99, Evalue=8e-21,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55721; Mature: 55721

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENTAKKFIITLDSGTTSCRSLIFDQKGRVISLVQKEFSQHFPRSGVEHDPIEIWNTQV
CCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCEE
YTMQAAKTKAGLKSTDFVALGLTNQRETVVLDKTNGEPVYNAIVWQDRRTSDFCDYLIEK
EEEEHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHH
GYQNYIKEVTGLIINPYFSGTKIRILKNVKKAKEVLAAGNLLAGTIDSLLKLTNGKVHAT
HHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEE
DITNASRTMLFDIKKRKDQKILDLLEIPYSILPKVLASADDYGFVSPFHSINAKTKVPIY
ECCCCCCEEEEEHHHHCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCEE
ALIGDQQSALFGQLATEFGMVKNTYGTGCFMLVNTGEKLIKSKNNLLTTVAQIGNNPIQY
EEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCHHHHHHHHHCCCCEEE
ALEGSVFIAGAAIQWLRDGLSLIENSSDCDSLVSKAKKNDYHIILVPSFTGLGAPYWDSY
EECCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
SRGAIFGLERGTKKEHLIKASLESIAFQANDLIKAIESDLGTKISLMKVDGGVSKSDYLM
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
QFQSSISQVEIQRPKNIETTAMGAAFLAGLYAKFWNSLAELKQILVPEKVFVPEFDQFMV
HHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
KKLTSNWDLAVRKTLNWKKDIK
HHHCCCCCEEEHHHCCCCCCCC
>Mature Secondary Structure
MLENTAKKFIITLDSGTTSCRSLIFDQKGRVISLVQKEFSQHFPRSGVEHDPIEIWNTQV
CCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCEE
YTMQAAKTKAGLKSTDFVALGLTNQRETVVLDKTNGEPVYNAIVWQDRRTSDFCDYLIEK
EEEEHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHH
GYQNYIKEVTGLIINPYFSGTKIRILKNVKKAKEVLAAGNLLAGTIDSLLKLTNGKVHAT
HHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEE
DITNASRTMLFDIKKRKDQKILDLLEIPYSILPKVLASADDYGFVSPFHSINAKTKVPIY
ECCCCCCEEEEEHHHHCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCEE
ALIGDQQSALFGQLATEFGMVKNTYGTGCFMLVNTGEKLIKSKNNLLTTVAQIGNNPIQY
EEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCHHHHHHHHHCCCCEEE
ALEGSVFIAGAAIQWLRDGLSLIENSSDCDSLVSKAKKNDYHIILVPSFTGLGAPYWDSY
EECCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
SRGAIFGLERGTKKEHLIKASLESIAFQANDLIKAIESDLGTKISLMKVDGGVSKSDYLM
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
QFQSSISQVEIQRPKNIETTAMGAAFLAGLYAKFWNSLAELKQILVPEKVFVPEFDQFMV
HHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
KKLTSNWDLAVRKTLNWKKDIK
HHHCCCCCEEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]