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The map label for this gene is glpF

Identifier: 54020479

GI number: 54020479

Start: 438175

End: 438921

Strand: Reverse

Name: glpF

Synonym: mhp369

Alternate gene names: 54020479

Gene position: 438921-438175 (Counterclockwise)

Preceding gene: 54020480

Following gene: 54020478

Centisome position: 49.16

GC content: 32.93

Gene sequence:

>747_bases
ATGAATAATCTCTTTTTTTTATCAGAATTTCTTGGGACTGCGATACTAATTTTACTCGGAAATGGCGTAAATTATAGTGT
AAATGCGGCTAAAATGTTTGCAAATCAATCCGGAAAATGAATTGTGATTGCACTAGGTTGAGCTCTTGGGGTGCTTTTAG
GAATAATTATTTCAAATGGAATTATCGAAAGTGCGCCGGCGCATCTAAATCCGGCAGTTTCAATATTTTTTGGCATTATT
TTTCAAAATAGTGAATTACTTGCGCTGATTCCTTTTCAGTTTTTAGGGGCAATTTTTGCTCAAGTTATTCTAAATTTTAT
CAACTGAAGTCATATAAAGGAAACAAAAGCAGAAATTATCGCTGCATGCCATCATACCAGCCCTGCTTTTAAAAAATCAT
ATATATCTAATTTTTTCTATGAATTTATCGGAACTACCGTTTTATTAGCAGCAATTTTTTTATTAAATAAAACTTTTTCA
ACAATGAAACCAGTAATTATTGCACTAATTGTGCTCTCACTTGGACTTTCACTAGGATCTTCGACTGGATATGCAGTTAA
TCCCGCCCGTGATTTTGGCCCAAGATTAGTTTATTTTCTTTTTGCTAATCTAGTTTTAAAAAGAAGAAAGGAATTTGCTG
CCATTAAAAATTGGCAAGAGATTTTTGGATTTTCTTATTCCTTAAATCCGATCATTGCCCCTAGTTTAGCAGGTCTAATT
CTCGGATTAGTTATTTATGCACTTTAG

Upstream 100 bases:

>100_bases
TTTGACCAATTTATGGTAAAAAAATTAACCTCCAATTGGGACTTAGCTGTTAGAAAAACGCTAAATTGGAAAAAAGATAT
TAAATAAAAAGGAATCCAAG

Downstream 100 bases:

>100_bases
ATAATTTTATCGACCATAAAACAAAAAAAGACTATAAATTTATAGTCTTTTTTTAAATTTTTAGTGATTTTATTTAGCTT
ATTAAAACCACAAAAAAGAA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNNLFFLSEFLGTAILILLGNGVNYSVNAAKMFANQSGKWIVIALGWALGVLLGIIISNGIIESAPAHLNPAVSIFFGII
FQNSELLALIPFQFLGAIFAQVILNFINWSHIKETKAEIIAACHHTSPAFKKSYISNFFYEFIGTTVLLAAIFLLNKTFS
TMKPVIIALIVLSLGLSLGSSTGYAVNPARDFGPRLVYFLFANLVLKRRKEFAAIKNWQEIFGFSYSLNPIIAPSLAGLI
LGLVIYAL

Sequences:

>Translated_248_residues
MNNLFFLSEFLGTAILILLGNGVNYSVNAAKMFANQSGK*IVIALG*ALGVLLGIIISNGIIESAPAHLNPAVSIFFGII
FQNSELLALIPFQFLGAIFAQVILNFIN*SHIKETKAEIIAACHHTSPAFKKSYISNFFYEFIGTTVLLAAIFLLNKTFS
TMKPVIIALIVLSLGLSLGSSTGYAVNPARDFGPRLVYFLFANLVLKRRKEFAAIKNWQEIFGFSYSLNPIIAPSLAGLI
LGLVIYAL
>Mature_248_residues
MNNLFFLSEFLGTAILILLGNGVNYSVNAAKMFANQSGK*IVIALG*ALGVLLGIIISNGIIESAPAHLNPAVSIFFGII
FQNSELLALIPFQFLGAIFAQVILNFIN*SHIKETKAEIIAACHHTSPAFKKSYISNFFYEFIGTTVLLAAIFLLNKTFS
TMKPVIIALIVLSLGLSLGSSTGYAVNPARDFGPRLVYFLFANLVLKRRKEFAAIKNWQEIFGFSYSLNPIIAPSLAGLI
LGLVIYAL

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=258, Percent_Identity=31.0077519379845, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI22538420, Length=260, Percent_Identity=28.0769230769231, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI157266307, Length=262, Percent_Identity=30.1526717557252, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1790362, Length=211, Percent_Identity=29.3838862559242, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71992966, Length=203, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=203, Percent_Identity=31.5270935960591, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 26527; Mature: 26527

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHC
>Mature Secondary Structure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HHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12949158 [H]