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The map label for this gene is pdhA

Identifier: 54020423

GI number: 54020423

Start: 289851

End: 290975

Strand: Reverse

Name: pdhA

Synonym: mhp265

Alternate gene names: 54020423

Gene position: 290975-289851 (Counterclockwise)

Preceding gene: 54020424

Following gene: 54020422

Centisome position: 32.59

GC content: 37.07

Gene sequence:

>1125_bases
ATGGACAAATTTCGCTATGTAAAGCCTGGTCAAATTATGGCAAAAGATGAAGAAATGATTCGCTTTCTTGATATTGATGG
TAATCTTTTATCTTCAACTGTTTTTGGACCAATCGACGAAACAAATGATATTCGCTTATCAAAACAGGAAATCAAAAAAG
CTTATGAATTTATGGTTTTATCTCGCCAACAAGATACGTATATGACACAACTACAGCGACAAGGTAGAATGTTGACTTTT
GCCCCTAACTTTGGTGAAGAAGCTCTTCAAGTAGCCTCAGGGATGGCGCTAACAAAAGATGACTGATTTGTCCCAGCTTT
TCGTTCAAATGCAACAATGTTATATCTTGGCGTGCCAATGATCTTGCAAATGCAATATTGAAATGGTAGCGAAAAAGGTA
ATGTAATTCCCGAAAATGTTAATGTTTTACCTATTAACATTCCGATCGGAACGCAGTTTTCCCATGCTGCCGGAATTGCT
TATGCAGCAAAACTAACAGGTAAAAAAATAGTTTCAATGAGTTTTATTGGAAACGGGGGGACTGCCGAAGGCGAGTTTTA
CGAAGCGCTAAATATTGCAAGTATTTGAAAATGACCAGTTGTTTTTTGCGTAAATAACAATCAATGAGCAATTTCAACCC
CAAATAAATATGAAAACGGTGCCTCAACAATTGCTGCAAAAGCAATGGCAGCCGGAATTCCTGGAATTCGTGTAGACGGA
AATGACCTTTTAGCTTCTTATGAAGTAATCAAGGAAGCTGTTGATTATGCTCGTTCTGGAAACGGTCCTGTTCTTGTTGA
GTTTGTAACTTGACGTCAAGGTGTTCATACCTCTTCTGATAATCCACGAATTTATCGTACTGTTGAAGAGGAAAAAGAAC
ACGAAAAATGAGAACCAATGCACCGGATTGAAAAATATATGTTTGACCGCGGAATTCTTGATTCTGCCGAAAAACAAAAA
ATTTGAGATGAAGCGCTTGCGATTGTCAAAGAAACTTATGAAAAATCTCTTGTTGGGCTTGAGTCAACAATTGATGAAAT
TTTCGATCATACCTACAAGGTTTTACCACCAGAACTTGAAGAACAAAAACAAGAAGCGCTTGAATTTTTTAAAGGAGTAA
AATAA

Upstream 100 bases:

>100_bases
TAATTTAAATGTAAGTTTATTTTTGAACTCGAGTGAAATTTTTTCTTATATTTTCTCGAGTTTTATAAAAACTACGAACA
AAATATAAAAAAGGAGATAT

Downstream 100 bases:

>100_bases
TGGCAAATTCAGATAAAATCGCTCTTAATAACATCGGTGCAGTTACAAATGCTCTTGATTTAATGATGGAAAAGGATTCT
CGCGTTGTTCTTTGGGGCGA

Product: pyruvate dehydrogenase E1-alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MDKFRYVKPGQIMAKDEEMIRFLDIDGNLLSSTVFGPIDETNDIRLSKQEIKKAYEFMVLSRQQDTYMTQLQRQGRMLTF
APNFGEEALQVASGMALTKDDWFVPAFRSNATMLYLGVPMILQMQYWNGSEKGNVIPENVNVLPINIPIGTQFSHAAGIA
YAAKLTGKKIVSMSFIGNGGTAEGEFYEALNIASIWKWPVVFCVNNNQWAISTPNKYENGASTIAAKAMAAGIPGIRVDG
NDLLASYEVIKEAVDYARSGNGPVLVEFVTWRQGVHTSSDNPRIYRTVEEEKEHEKWEPMHRIEKYMFDRGILDSAEKQK
IWDEALAIVKETYEKSLVGLESTIDEIFDHTYKVLPPELEEQKQEALEFFKGVK

Sequences:

>Translated_374_residues
MDKFRYVKPGQIMAKDEEMIRFLDIDGNLLSSTVFGPIDETNDIRLSKQEIKKAYEFMVLSRQQDTYMTQLQRQGRMLTF
APNFGEEALQVASGMALTKDD*FVPAFRSNATMLYLGVPMILQMQY*NGSEKGNVIPENVNVLPINIPIGTQFSHAAGIA
YAAKLTGKKIVSMSFIGNGGTAEGEFYEALNIASI*K*PVVFCVNNNQ*AISTPNKYENGASTIAAKAMAAGIPGIRVDG
NDLLASYEVIKEAVDYARSGNGPVLVEFVT*RQGVHTSSDNPRIYRTVEEEKEHEK*EPMHRIEKYMFDRGILDSAEKQK
I*DEALAIVKETYEKSLVGLESTIDEIFDHTYKVLPPELEEQKQEALEFFKGVK
>Mature_374_residues
MDKFRYVKPGQIMAKDEEMIRFLDIDGNLLSSTVFGPIDETNDIRLSKQEIKKAYEFMVLSRQQDTYMTQLQRQGRMLTF
APNFGEEALQVASGMALTKDD*FVPAFRSNATMLYLGVPMILQMQY*NGSEKGNVIPENVNVLPINIPIGTQFSHAAGIA
YAAKLTGKKIVSMSFIGNGGTAEGEFYEALNIASI*K*PVVFCVNNNQ*AISTPNKYENGASTIAAKAMAAGIPGIRVDG
NDLLASYEVIKEAVDYARSGNGPVLVEFVT*RQGVHTSSDNPRIYRTVEEEKEHEK*EPMHRIEKYMFDRGILDSAEKQK
I*DEALAIVKETYEKSLVGLESTIDEIFDHTYKVLPPELEEQKQEALEFFKGVK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11386135, Length=377, Percent_Identity=31.8302387267904, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI258645172, Length=377, Percent_Identity=31.5649867374005, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI291084742, Length=322, Percent_Identity=27.3291925465839, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4505685, Length=322, Percent_Identity=27.3291925465839, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI291084744, Length=210, Percent_Identity=32.3809523809524, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI4885543, Length=324, Percent_Identity=27.1604938271605, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI291084757, Length=187, Percent_Identity=31.5508021390374, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI86563357, Length=342, Percent_Identity=32.4561403508772, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI86563355, Length=342, Percent_Identity=32.4561403508772, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17536047, Length=302, Percent_Identity=28.476821192053, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32564172, Length=303, Percent_Identity=28.3828382838284, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321026, Length=292, Percent_Identity=27.0547945205479, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355903, Length=362, Percent_Identity=30.3867403314917, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24639740, Length=341, Percent_Identity=25.8064516129032, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24639744, Length=341, Percent_Identity=25.8064516129032, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI28571106, Length=341, Percent_Identity=25.8064516129032, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24639746, Length=291, Percent_Identity=26.1168384879725, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24639748, Length=321, Percent_Identity=24.2990654205607, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017596 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 40692; Mature: 40692

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFRYVKPGQIMAKDEEMIRFLDIDGNLLSSTVFGPIDETNDIRLSKQEIKKAYEFMVL
CCCCCCCCCCCEEECCCCEEEEECCCCCHHHHCCCCCCCCCCCCEECHHHHHHHHHHHHH
SRQQDTYMTQLQRQGRMLTFAPNFGEEALQVASGMALTKDDFVPAFRSNATMLYLGVPMI
CCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCEECCCCCCHHHHCCCEEEEECCCEE
LQMQYNGSEKGNVIPENVNVLPINIPIGTQFSHAAGIAYAAKLTGKKIVSMSFIGNGGTA
EEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCC
EGEFYEALNIASIKPVVFCVNNNQAISTPNKYENGASTIAAKAMAAGIPGIRVDGNDLLA
CCHHHHHHCCCCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHH
SYEVIKEAVDYARSGNGPVLVEFVTRQGVHTSSDNPRIYRTVEEEKEHEKEPMHRIEKYM
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHH
FDRGILDSAEKQKIDEALAIVKETYEKSLVGLESTIDEIFDHTYKVLPPELEEQKQEALE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FFKGVK
HHHCCC
>Mature Secondary Structure
MDKFRYVKPGQIMAKDEEMIRFLDIDGNLLSSTVFGPIDETNDIRLSKQEIKKAYEFMVL
CCCCCCCCCCCEEECCCCEEEEECCCCCHHHHCCCCCCCCCCCCEECHHHHHHHHHHHHH
SRQQDTYMTQLQRQGRMLTFAPNFGEEALQVASGMALTKDDFVPAFRSNATMLYLGVPMI
CCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCEECCCCCCHHHHCCCEEEEECCCEE
LQMQYNGSEKGNVIPENVNVLPINIPIGTQFSHAAGIAYAAKLTGKKIVSMSFIGNGGTA
EEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCC
EGEFYEALNIASIKPVVFCVNNNQAISTPNKYENGASTIAAKAMAAGIPGIRVDGNDLLA
CCHHHHHHCCCCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHH
SYEVIKEAVDYARSGNGPVLVEFVTRQGVHTSSDNPRIYRTVEEEKEHEKEPMHRIEKYM
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHH
FDRGILDSAEKQKIDEALAIVKETYEKSLVGLESTIDEIFDHTYKVLPPELEEQKQEALE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FFKGVK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]