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The map label for this gene is pdhB

Identifier: 54020422

GI number: 54020422

Start: 288847

End: 289851

Strand: Reverse

Name: pdhB

Synonym: mhp264

Alternate gene names: 54020422

Gene position: 289851-288847 (Counterclockwise)

Preceding gene: 54020423

Following gene: 54020421

Centisome position: 32.47

GC content: 39.0

Gene sequence:

>1005_bases
ATGGCAAATTCAGATAAAATCGCTCTTAATAACATCGGTGCAGTTACAAATGCTCTTGATTTAATGATGGAAAAGGATTC
TCGCGTTGTTCTTTGGGGCGAAGATGCCGGATTTGAAGGTGGGGTTTTTCGAGCAACTGAAGGTCTTCAAAAGAAATACG
GGATTGAACGCGTCTGAGATTCGCCAATTGCTGAGGCTTCAATTTGTGGAGTTGGAGTCGGAGCAGCAATTGCTGGACTT
CGTCCAGTCGTTGAAATGCAGTTTCAAGGATTTTCCTATCCTGCATTTCAACAACTTTTTGTTCATGCAGCTCGTTATCG
AAACCGTTCTCGCAGTCGGTTTAGTGTACCAATGGTAATGCGAATGCCGATGGCTGGGGGTGTTAGGGCTCTTGAACACC
ATTCTGAAGCAATTGAAGCACTTTTTGCACATATTCCTGGACTAAAAGTGGTGATGCCTTCAACCCCTTATGATACAAAG
GGACTTTTAATTGCCGCAATTAATGACCCGGATCCAGTTGTATTTCTTGAACCAAAAAAAATCTATCGTGCTTTCAAACA
AGAAGTTCCAGCCGGAATTTACGAGGTTCCAATCGGAAAAGCGAATGTTATAAAAGAAGGTTCAGATCTTACTCTGGTAA
CTTATGGGGCACAAGTTCATGAAGCAATTGCCGCAATTCAACAACTTCCAAATACAAAGGGACTTGAAGAAGTTGATGTT
GAACTAATTGATCTAAGAACTATTAAACCATTGGATACTGAAACAATTATTGAATCTGTTAAAAAAACTGGAAGAATTTT
AATTGTTCATGAAGCAGTTAAATCCTTTTCGGTTTCAGCTGAAATTATTTCCCGTGTAAATGAAAAAGCATTCGAGTATC
TCGAATCTGCACCTGCTCGACTTACCGGTTATGATATTACTGTTCCACTTGCAAAAGGTGAGAATTTCCATTCAATAACA
AAAGAAAAAATTATTGACAAAATTCGCAAAATTATGGAATCATAA

Upstream 100 bases:

>100_bases
CAACAATTGATGAAATTTTCGATCATACCTACAAGGTTTTACCACCAGAACTTGAAGAACAAAAACAAGAAGCGCTTGAA
TTTTTTAAAGGAGTAAAATA

Downstream 100 bases:

>100_bases
TTTAATTTCGTTTAAAAACTTAATAAAAACATTCAGAATTCAATTTTTTAATTCTGAATGTTTTTTTTTTTTTTTTTTTT
TTAAGATGAAGAATAAATAA

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MANSDKIALNNIGAVTNALDLMMEKDSRVVLWGEDAGFEGGVFRATEGLQKKYGIERVWDSPIAEASICGVGVGAAIAGL
RPVVEMQFQGFSYPAFQQLFVHAARYRNRSRSRFSVPMVMRMPMAGGVRALEHHSEAIEALFAHIPGLKVVMPSTPYDTK
GLLIAAINDPDPVVFLEPKKIYRAFKQEVPAGIYEVPIGKANVIKEGSDLTLVTYGAQVHEAIAAIQQLPNTKGLEEVDV
ELIDLRTIKPLDTETIIESVKKTGRILIVHEAVKSFSVSAEIISRVNEKAFEYLESAPARLTGYDITVPLAKGENFHSIT
KEKIIDKIRKIMES

Sequences:

>Translated_334_residues
MANSDKIALNNIGAVTNALDLMMEKDSRVVLWGEDAGFEGGVFRATEGLQKKYGIERV*DSPIAEASICGVGVGAAIAGL
RPVVEMQFQGFSYPAFQQLFVHAARYRNRSRSRFSVPMVMRMPMAGGVRALEHHSEAIEALFAHIPGLKVVMPSTPYDTK
GLLIAAINDPDPVVFLEPKKIYRAFKQEVPAGIYEVPIGKANVIKEGSDLTLVTYGAQVHEAIAAIQQLPNTKGLEEVDV
ELIDLRTIKPLDTETIIESVKKTGRILIVHEAVKSFSVSAEIISRVNEKAFEYLESAPARLTGYDITVPLAKGENFHSIT
KEKIIDKIRKIMES
>Mature_333_residues
ANSDKIALNNIGAVTNALDLMMEKDSRVVLWGEDAGFEGGVFRATEGLQKKYGIERV*DSPIAEASICGVGVGAAIAGLR
PVVEMQFQGFSYPAFQQLFVHAARYRNRSRSRFSVPMVMRMPMAGGVRALEHHSEAIEALFAHIPGLKVVMPSTPYDTKG
LLIAAINDPDPVVFLEPKKIYRAFKQEVPAGIYEVPIGKANVIKEGSDLTLVTYGAQVHEAIAAIQQLPNTKGLEEVDVE
LIDLRTIKPLDTETIIESVKKTGRILIVHEAVKSFSVSAEIISRVNEKAFEYLESAPARLTGYDITVPLAKGENFHSITK
EKIIDKIRKIMES

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=325, Percent_Identity=43.6923076923077, Blast_Score=258, Evalue=4e-69,
Organism=Homo sapiens, GI34101272, Length=325, Percent_Identity=43.6923076923077, Blast_Score=258, Evalue=4e-69,
Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=37.3456790123457, Blast_Score=198, Evalue=7e-51,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=36.7283950617284, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17506935, Length=331, Percent_Identity=41.6918429003021, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17538422, Length=322, Percent_Identity=36.6459627329193, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319698, Length=324, Percent_Identity=36.4197530864198, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI160714832, Length=326, Percent_Identity=38.6503067484663, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI160714828, Length=326, Percent_Identity=38.6503067484663, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI21358145, Length=324, Percent_Identity=38.5802469135802, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24650940, Length=324, Percent_Identity=38.5802469135802, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24650943, Length=82, Percent_Identity=45.1219512195122, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24650945, Length=82, Percent_Identity=45.1219512195122, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36531; Mature: 36400

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSDKIALNNIGAVTNALDLMMEKDSRVVLWGEDAGFEGGVFRATEGLQKKYGIERVDS
CCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHHCCHHHHCC
PIAEASICGVGVGAAIAGLRPVVEMQFQGFSYPAFQQLFVHAARYRNRSRSRFSVPMVMR
CHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHEE
MPMAGGVRALEHHSEAIEALFAHIPGLKVVMPSTPYDTKGLLIAAINDPDPVVFLEPKKI
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCEEEECHHHH
YRAFKQEVPAGIYEVPIGKANVIKEGSDLTLVTYGAQVHEAIAAIQQLPNTKGLEEVDVE
HHHHHHHCCCCEEECCCCCCHHEECCCCEEEEEECHHHHHHHHHHHHCCCCCCCHHHCEE
LIDLRTIKPLDTETIIESVKKTGRILIVHEAVKSFSVSAEIISRVNEKAFEYLESAPARL
EEEEECCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
TGYDITVPLAKGENFHSITKEKIIDKIRKIMES
ECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANSDKIALNNIGAVTNALDLMMEKDSRVVLWGEDAGFEGGVFRATEGLQKKYGIERVDS
CCCCCEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHHCCHHHHCC
PIAEASICGVGVGAAIAGLRPVVEMQFQGFSYPAFQQLFVHAARYRNRSRSRFSVPMVMR
CHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHEE
MPMAGGVRALEHHSEAIEALFAHIPGLKVVMPSTPYDTKGLLIAAINDPDPVVFLEPKKI
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCEEEECHHHH
YRAFKQEVPAGIYEVPIGKANVIKEGSDLTLVTYGAQVHEAIAAIQQLPNTKGLEEVDVE
HHHHHHHCCCCEEECCCCCCHHEECCCCEEEEEECHHHHHHHHHHHHCCCCCCCHHHCEE
LIDLRTIKPLDTETIIESVKKTGRILIVHEAVKSFSVSAEIISRVNEKAFEYLESAPARL
EEEEECCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
TGYDITVPLAKGENFHSITKEKIIDKIRKIMES
ECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1735725 [H]