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The map label for this gene is tilS [H]

Identifier: 54020383

GI number: 54020383

Start: 215319

End: 216212

Strand: Reverse

Name: tilS [H]

Synonym: mhp176

Alternate gene names: 54020383

Gene position: 216212-215319 (Counterclockwise)

Preceding gene: 54020384

Following gene: 54020382

Centisome position: 24.22

GC content: 23.6

Gene sequence:

>894_bases
ATGCAAATAAAAATAAAGTCTAAAGAAAAATATTTAATAGGTGTCTCAGGTGGCTCTGATTCAATGTTTTTGCTCAATAA
ATATAAAAATCAGGATGTAATTGTAGCTCATATAAATTACAATTTGCGCCCTGAAGCAATTTTTGAGACACTTTTGGTTT
CAAAATTTTGTCAAAAATACAATTTAGAGCTGAAAATTCTATCATTTGATAGTTTTAAGATTAAAAAAAATTTACAAAGC
GGGCTTAGGCTGGGCCGTTATCAATTTTTTGAAAAAATTTATAAAGAATTTAATTGTACTAAATTATTGGTTGGTCACCA
CCGTGATGATTTTCTTGAGACAGTTTTTTTGCAGAAAAAACAGAAAAAAATTGTCACTTTTTGAGGAATACATAAAAAAA
ATAACCTTTTTAATATGGAGATTTTGCGTCCTTTTTTGTATTGGAGAACAAAAAAACAAATTATTCGAATTTGTCAACAA
AAAAAAATTCCCTATCTTGATGATCAATCAAATTTTACGGGCAAATATCAACGTAATCAAATCAGGTTTTTATTGGAAAA
GAAGAGTGATTTTTCACTTTTTTTTCTTTTTTTATTTTATTATTTAATAAACATATTTAAATTAATAATATTAAAAAATC
AGAAAAAAATTTTGCAAAATTGACAGAAAACAGGGTATAATATAAATTTCTTTAAGAAGATAAAAATTAAGTCAAAAATT
ATTTTTTTATTTGTTAATCAAAATTTTGATAATGTTAAATTAACTAGGGGGAAAATTAACGAAATTATTAATTTTATTTG
CGGGAAATCGACTAGTGGGGCTTTTTTACTAAAAAAAAACAATTATATTATAAAAAAAAAATGAAAAATATTGCCAAAAT
CTAGTAAAATTTAG

Upstream 100 bases:

>100_bases
CCTAAAGATAATGCAAAAATTAAACTTGTAATTGAAGAAGCAGCTAATATTCTGATTTTTTATTTATCTAATAGTTTTAT
TACAACAATCGAAAAATTTA

Downstream 100 bases:

>100_bases
TAATAATTTGTATTTTATTTATAAACTGGAGGGTAAATTATGGCAGCGAAAACAAGAAAAAAACCTTCAATTGGCCTGAT
AATTTTTTTAGTAATTACTT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MQIKIKSKEKYLIGVSGGSDSMFLLNKYKNQDVIVAHINYNLRPEAIFETLLVSKFCQKYNLELKILSFDSFKIKKNLQS
GLRLGRYQFFEKIYKEFNCTKLLVGHHRDDFLETVFLQKKQKKIVTFWGIHKKNNLFNMEILRPFLYWRTKKQIIRICQQ
KKIPYLDDQSNFTGKYQRNQIRFLLEKKSDFSLFFLFLFYYLINIFKLIILKNQKKILQNWQKTGYNINFFKKIKIKSKI
IFLFVNQNFDNVKLTRGKINEIINFICGKSTSGAFLLKKNNYIIKKKWKILPKSSKI

Sequences:

>Translated_297_residues
MQIKIKSKEKYLIGVSGGSDSMFLLNKYKNQDVIVAHINYNLRPEAIFETLLVSKFCQKYNLELKILSFDSFKIKKNLQS
GLRLGRYQFFEKIYKEFNCTKLLVGHHRDDFLETVFLQKKQKKIVTF*GIHKKNNLFNMEILRPFLYWRTKKQIIRICQQ
KKIPYLDDQSNFTGKYQRNQIRFLLEKKSDFSLFFLFLFYYLINIFKLIILKNQKKILQN*QKTGYNINFFKKIKIKSKI
IFLFVNQNFDNVKLTRGKINEIINFICGKSTSGAFLLKKNNYIIKKK*KILPKSSKI
>Mature_297_residues
MQIKIKSKEKYLIGVSGGSDSMFLLNKYKNQDVIVAHINYNLRPEAIFETLLVSKFCQKYNLELKILSFDSFKIKKNLQS
GLRLGRYQFFEKIYKEFNCTKLLVGHHRDDFLETVFLQKKQKKIVTF*GIHKKNNLFNMEILRPFLYWRTKKQIIRICQQ
KKIPYLDDQSNFTGKYQRNQIRFLLEKKSDFSLFFLFLFYYLINIFKLIILKNQKKILQN*QKTGYNINFFKKIKIKSKI
IFLFVNQNFDNVKLTRGKINEIINFICGKSTSGAFLLKKNNYIIKKK*KILPKSSKI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35123; Mature: 35123

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKIKSKEKYLIGVSGGSDSMFLLNKYKNQDVIVAHINYNLRPEAIFETLLVSKFCQKY
CEEEEECCCEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
NLELKILSFDSFKIKKNLQSGLRLGRYQFFEKIYKEFNCTKLLVGHHRDDFLETVFLQKK
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
QKKIVTFGIHKKNNLFNMEILRPFLYWRTKKQIIRICQQKKIPYLDDQSNFTGKYQRNQI
CCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCE
RFLLEKKSDFSLFFLFLFYYLINIFKLIILKNQKKILQNQKTGYNINFFKKIKIKSKIIF
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEEEEEEEEEEEE
LFVNQNFDNVKLTRGKINEIINFICGKSTSGAFLLKKNNYIIKKKKILPKSSKI
EEEECCCCCEEEEHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MQIKIKSKEKYLIGVSGGSDSMFLLNKYKNQDVIVAHINYNLRPEAIFETLLVSKFCQKY
CEEEEECCCEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
NLELKILSFDSFKIKKNLQSGLRLGRYQFFEKIYKEFNCTKLLVGHHRDDFLETVFLQKK
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
QKKIVTFGIHKKNNLFNMEILRPFLYWRTKKQIIRICQQKKIPYLDDQSNFTGKYQRNQI
CCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCE
RFLLEKKSDFSLFFLFLFYYLINIFKLIILKNQKKILQNQKTGYNINFFKKIKIKSKIIF
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEEEEEEEEEEEE
LFVNQNFDNVKLTRGKINEIINFICGKSTSGAFLLKKNNYIIKKKKILPKSSKI
EEEECCCCCEEEEHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA