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The map label for this gene is eno

Identifier: 54020356

GI number: 54020356

Start: 149580

End: 150938

Strand: Reverse

Name: eno

Synonym: mhp129

Alternate gene names: 54020356

Gene position: 150938-149580 (Counterclockwise)

Preceding gene: 54020357

Following gene: 54020355

Centisome position: 16.91

GC content: 34.44

Gene sequence:

>1359_bases
ATGTCAAAAATTACTAAAGTTTTTGCTCGCGAAATTTTAGATTCCCGCGGAAATCCAACAATTCAAGTTGATGTTTATAC
TCTAGCAGGTGGATTTGGTTCAGCAATTGTTCCATCAGGAGCATCGACTGGTTCCCGAGAAGCGCTTGAATTACGTGATA
CAAATACAAAATATGCAGATAATTGATATGGTCAAAAAGGCGTAATGACCGCTGTGGATAATGTAAATAATATAATAGCA
CCAGAAATTATTGGACTTTGTTGTAAAAATCAAAGATTAATCGATCAAAAAATGATTGAATTAGACGGGACTCCAAATAA
AGAAAAATTAGGTGCTAATGCAATTTTAGGGGTTTCACTTGCAGTTGCAAAAGCTGCAGCAAACGAGTTAAGAATGCCAC
TTTTCCGTTATTTAGGAGGAACCAATCCAACTTTAATGCCAGTTCCAATGCTTAATGTTATAAATGGGGGAGAACATGCC
TCAAATACACTTGATTTTCAAGAGTTTATGATAATGCCACTTGGTTTTAGAACTTTTAAAGAGGCATTACAAGCGGCAAA
TAAGGTTTTTCATAATTTAGCAAAACTTCTTAAAAAATCAGGTTTTGAAACGCAAGTAGGCGATGAAGGAGGGTTTGCCC
CTAACTTTAATTCCCACGAACAAGCGCTTGATTTTTTAGTTGATGCGATCAAGGAATCCGGATTTAATCCCGGATTTAAA
GGGGAAAATGCTGTTGCAATTGCAATTGATGCAGCTGCTTCTGAATTTTATAATGGGCAAAAATATGTCTTTAAGAAACT
AAAGGCTGCCTCTTTAAGTAAAAACCAGGCAGATTTAGATGAAAAATTTGAATTTAGTTCAGAGGAATTACTTAATTATT
ATGGTCAACTTTTAGCAAAATATCCAATAATTTCAATAGAAGATGGATTTGCTGAATCTGATTGACAGGGTTTTATCGCC
TTTAATCAAAAATATGGAAATAATCACCAGATTGTCGGCGATGACTTAACTGTTACAAATGTAGAAATCCTCAAAAAAGC
TATAAATTTAAAAGCAATTAATTCAATTTTAATCAAATTAAACCAAATAGGAACCTTAAGTGAAACACTTGATGCAATTC
ATTTAGCACAAAAATCTGGGATGACCGCTGTAATTTCCCATCGTTCAGGAGAATCTGAGGATACAACAATCGCCGATCTT
GCGGTTGCAGTATCTTCAGGACAAATTAAAACAGGTTCACTTTCCAGAACGGATCGGATTGCAAAGTATAACCGACTTTT
AGTAATCGAGGAATATCTAAATTCTTATGCAAAAGCCGATTATATTGGTAGGGAAGTTTTTTATAACTTAAAAAAATAG

Upstream 100 bases:

>100_bases
AATAATTTAATTTTTAAATTATGGTATAATTTTGTACATTCTAAGTTTTTAGTATTAATTTAAGATTTGAATGAATAAAA
AATAAAATAGAGAGGTTATT

Downstream 100 bases:

>100_bases
TCTAAATTATGAATATTCGTTTAATTTTGAATAATAAATCCTTTGTTGAAAAAAAACTCGCAACCCGTGGTTTTGATATT
TCAATAATTAACGAATTATA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSKITKVFAREILDSRGNPTIQVDVYTLAGGFGSAIVPSGASTGSREALELRDTNTKYADNWYGQKGVMTAVDNVNNIIA
PEIIGLCCKNQRLIDQKMIELDGTPNKEKLGANAILGVSLAVAKAAANELRMPLFRYLGGTNPTLMPVPMLNVINGGEHA
SNTLDFQEFMIMPLGFRTFKEALQAANKVFHNLAKLLKKSGFETQVGDEGGFAPNFNSHEQALDFLVDAIKESGFNPGFK
GENAVAIAIDAAASEFYNGQKYVFKKLKAASLSKNQADLDEKFEFSSEELLNYYGQLLAKYPIISIEDGFAESDWQGFIA
FNQKYGNNHQIVGDDLTVTNVEILKKAINLKAINSILIKLNQIGTLSETLDAIHLAQKSGMTAVISHRSGESEDTTIADL
AVAVSSGQIKTGSLSRTDRIAKYNRLLVIEEYLNSYAKADYIGREVFYNLKK

Sequences:

>Translated_452_residues
MSKITKVFAREILDSRGNPTIQVDVYTLAGGFGSAIVPSGASTGSREALELRDTNTKYADN*YGQKGVMTAVDNVNNIIA
PEIIGLCCKNQRLIDQKMIELDGTPNKEKLGANAILGVSLAVAKAAANELRMPLFRYLGGTNPTLMPVPMLNVINGGEHA
SNTLDFQEFMIMPLGFRTFKEALQAANKVFHNLAKLLKKSGFETQVGDEGGFAPNFNSHEQALDFLVDAIKESGFNPGFK
GENAVAIAIDAAASEFYNGQKYVFKKLKAASLSKNQADLDEKFEFSSEELLNYYGQLLAKYPIISIEDGFAESD*QGFIA
FNQKYGNNHQIVGDDLTVTNVEILKKAINLKAINSILIKLNQIGTLSETLDAIHLAQKSGMTAVISHRSGESEDTTIADL
AVAVSSGQIKTGSLSRTDRIAKYNRLLVIEEYLNSYAKADYIGREVFYNLKK
>Mature_451_residues
SKITKVFAREILDSRGNPTIQVDVYTLAGGFGSAIVPSGASTGSREALELRDTNTKYADN*YGQKGVMTAVDNVNNIIAP
EIIGLCCKNQRLIDQKMIELDGTPNKEKLGANAILGVSLAVAKAAANELRMPLFRYLGGTNPTLMPVPMLNVINGGEHAS
NTLDFQEFMIMPLGFRTFKEALQAANKVFHNLAKLLKKSGFETQVGDEGGFAPNFNSHEQALDFLVDAIKESGFNPGFKG
ENAVAIAIDAAASEFYNGQKYVFKKLKAASLSKNQADLDEKFEFSSEELLNYYGQLLAKYPIISIEDGFAESD*QGFIAF
NQKYGNNHQIVGDDLTVTNVEILKKAINLKAINSILIKLNQIGTLSETLDAIHLAQKSGMTAVISHRSGESEDTTIADLA
VAVSSGQIKTGSLSRTDRIAKYNRLLVIEEYLNSYAKADYIGREVFYNLKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=454, Percent_Identity=48.2378854625551, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI301897469, Length=454, Percent_Identity=48.2378854625551, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI4503571, Length=447, Percent_Identity=48.0984340044743, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI5803011, Length=441, Percent_Identity=47.1655328798186, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI301897479, Length=452, Percent_Identity=44.6902654867257, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI169201331, Length=354, Percent_Identity=28.5310734463277, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI169201757, Length=354, Percent_Identity=28.5310734463277, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI239744207, Length=354, Percent_Identity=28.5310734463277, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1789141, Length=449, Percent_Identity=53.6748329621381, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71995829, Length=451, Percent_Identity=47.2283813747228, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17536383, Length=451, Percent_Identity=47.2283813747228, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32563855, Length=208, Percent_Identity=42.3076923076923, Blast_Score=155, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6324974, Length=439, Percent_Identity=48.0637813211845, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324969, Length=439, Percent_Identity=48.0637813211845, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6323985, Length=439, Percent_Identity=48.0637813211845, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321693, Length=442, Percent_Identity=47.2850678733032, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6321968, Length=456, Percent_Identity=46.7105263157895, Blast_Score=333, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24580918, Length=442, Percent_Identity=46.606334841629, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24580916, Length=442, Percent_Identity=46.606334841629, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24580920, Length=442, Percent_Identity=46.606334841629, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24580914, Length=442, Percent_Identity=46.606334841629, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI281360527, Length=442, Percent_Identity=46.606334841629, Blast_Score=344, Evalue=7e-95,
Organism=Drosophila melanogaster, GI17137654, Length=442, Percent_Identity=46.606334841629, Blast_Score=344, Evalue=7e-95,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 49045; Mature: 48914

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITKVFAREILDSRGNPTIQVDVYTLAGGFGSAIVPSGASTGSREALELRDTNTKYAD
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCHHH
NYGQKGVMTAVDNVNNIIAPEIIGLCCKNQRLIDQKMIELDGTPNKEKLGANAILGVSLA
CCCCCCHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHH
VAKAAANELRMPLFRYLGGTNPTLMPVPMLNVINGGEHASNTLDFQEFMIMPLGFRTFKE
HHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHH
ALQAANKVFHNLAKLLKKSGFETQVGDEGGFAPNFNSHEQALDFLVDAIKESGFNPGFKG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ENAVAIAIDAAASEFYNGQKYVFKKLKAASLSKNQADLDEKFEFSSEELLNYYGQLLAKY
CCEEEEEEEHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHC
PIISIEDGFAESDQGFIAFNQKYGNNHQIVGDDLTVTNVEILKKAINLKAINSILIKLNQ
CEEEECCCCCCCCCCEEEEECCCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
IGTLSETLDAIHLAQKSGMTAVISHRSGESEDTTIADLAVAVSSGQIKTGSLSRTDRIAK
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHEEECCCCEECCCCCHHHHHHH
YNRLLVIEEYLNSYAKADYIGREVFYNLKK
HHHHEEEHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKITKVFAREILDSRGNPTIQVDVYTLAGGFGSAIVPSGASTGSREALELRDTNTKYAD
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCHHH
NYGQKGVMTAVDNVNNIIAPEIIGLCCKNQRLIDQKMIELDGTPNKEKLGANAILGVSLA
CCCCCCHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHH
VAKAAANELRMPLFRYLGGTNPTLMPVPMLNVINGGEHASNTLDFQEFMIMPLGFRTFKE
HHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHH
ALQAANKVFHNLAKLLKKSGFETQVGDEGGFAPNFNSHEQALDFLVDAIKESGFNPGFKG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ENAVAIAIDAAASEFYNGQKYVFKKLKAASLSKNQADLDEKFEFSSEELLNYYGQLLAKY
CCEEEEEEEHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHC
PIISIEDGFAESDQGFIAFNQKYGNNHQIVGDDLTVTNVEILKKAINLKAINSILIKLNQ
CEEEECCCCCCCCCCEEEEECCCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
IGTLSETLDAIHLAQKSGMTAVISHRSGESEDTTIADLAVAVSSGQIKTGSLSRTDRIAK
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHEEECCCCEECCCCCHHHHHHH
YNRLLVIEEYLNSYAKADYIGREVFYNLKK
HHHHEEEHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA