Click here to switch to the map view.

The map label for this gene is aceF

Identifier: 54020221

GI number: 54020221

Start: 634345

End: 635265

Strand: Direct

Name: aceF

Synonym: mhp503

Alternate gene names: 54020221

Gene position: 634345-635265 (Clockwise)

Preceding gene: 54020220

Following gene: 54020222

Centisome position: 71.05

GC content: 36.37

Gene sequence:

>921_bases
ATGTCAAAAATTTTAGCAACCCCAAAAGCAAGAGCGATGGCAAAACATGCTAATATTGATCTTGCTGATCTTAAAATTCA
ACATCGGAAAATTGAAGCTTCCGATGTTGAAAATTACATAAAATCCTTAAAATCAGCACAAACTAGTGCTGAAAAAGTAG
AAGTTTCATCGTCTCAGCAGGCTGAAAAAACTTCTATTCCCGCGTCAATTCCGCCTAAATTAGAAGGAAAACGCGAGAAA
ATCGCTCCAATTCGAAAAGCAATTGCAAGAGCGATGACTAATTCTTGAAATTCAGTTGCCTATGTCAACCTCGTTAATCA
AATTGACGTTAGCGATCTTTGAAAACTTAGAAAATCAGTTTTAGACCCTGTCCTAAAAACATCAGGTGTGAAACTAACAT
TTCTTGCTTTTATTGCCAAAGCCATTTTGATTGCTCTTAGTGAATTTCCTGTTATGGCAGCAAAATATGATGAAGCAACA
AGTGAAATTGTCTATCCTGATACTTTAAATTTAGGTCTTGCTGTCGATACTGACGCCGGGCTTATGGTTCCAGTAATTAA
GGATGCTCAAAAGCTTTCAATTGTAGAAATTGCAAAAGAAATTGTTCGTCTTGCCAAAGCTGCCCGTGAACGTAAAATTA
AACCAAGCGAAATGCAAGGTGGATCATTTACAATCACAAATTATGGATCGGTTGGTTCACTTTATGGTGTTCCAGTAATA
AATTATCCTGAACTGGCAATTGCTGGAGTAGGAGCTATTATTGATTCAGCCGAGGTCAAGGATGGTCAAATTGTTGCTTC
AAAAATTATGCATTTAACAGTTGCTGCAGATCATCGTTGAATTGATGGTGCAACAATTGGTCGTTTTGCTGCAAGAGTTA
AAGAATTATTAGAAAAACCAGAAATTTTAGGAATCTTATAA

Upstream 100 bases:

>100_bases
CCTTTTGTTTGGCCGTTTTTAATATATAATTATAATGTATATTTCTAATTATGAAAAACCTTATAATTTATAGTATATTT
TAATAACTAGGGAAAACAAT

Downstream 100 bases:

>100_bases
TGTATAAATTTAAATTTGCTGATATCGGTGAGGGACTACATGAAGGTGTTGTTGCCCAAATTTATAAAAAAGAAGGGGAA
ACAGTCAATGAAGGTGATTC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSKILATPKARAMAKHANIDLADLKIQHRKIEASDVENYIKSLKSAQTSAEKVEVSSSQQAEKTSIPASIPPKLEGKREK
IAPIRKAIARAMTNSWNSVAYVNLVNQIDVSDLWKLRKSVLDPVLKTSGVKLTFLAFIAKAILIALSEFPVMAAKYDEAT
SEIVYPDTLNLGLAVDTDAGLMVPVIKDAQKLSIVEIAKEIVRLAKAARERKIKPSEMQGGSFTITNYGSVGSLYGVPVI
NYPELAIAGVGAIIDSAEVKDGQIVASKIMHLTVAADHRWIDGATIGRFAARVKELLEKPEILGIL

Sequences:

>Translated_306_residues
MSKILATPKARAMAKHANIDLADLKIQHRKIEASDVENYIKSLKSAQTSAEKVEVSSSQQAEKTSIPASIPPKLEGKREK
IAPIRKAIARAMTNS*NSVAYVNLVNQIDVSDL*KLRKSVLDPVLKTSGVKLTFLAFIAKAILIALSEFPVMAAKYDEAT
SEIVYPDTLNLGLAVDTDAGLMVPVIKDAQKLSIVEIAKEIVRLAKAARERKIKPSEMQGGSFTITNYGSVGSLYGVPVI
NYPELAIAGVGAIIDSAEVKDGQIVASKIMHLTVAADHR*IDGATIGRFAARVKELLEKPEILGIL
>Mature_305_residues
SKILATPKARAMAKHANIDLADLKIQHRKIEASDVENYIKSLKSAQTSAEKVEVSSSQQAEKTSIPASIPPKLEGKREKI
APIRKAIARAMTNS*NSVAYVNLVNQIDVSDL*KLRKSVLDPVLKTSGVKLTFLAFIAKAILIALSEFPVMAAKYDEATS
EIVYPDTLNLGLAVDTDAGLMVPVIKDAQKLSIVEIAKEIVRLAKAARERKIKPSEMQGGSFTITNYGSVGSLYGVPVIN
YPELAIAGVGAIIDSAEVKDGQIVASKIMHLTVAADHR*IDGATIGRFAARVKELLEKPEILGIL

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=310, Percent_Identity=34.5161290322581, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=35.3982300884956, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI31711992, Length=303, Percent_Identity=28.3828382838284, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI203098816, Length=223, Percent_Identity=31.390134529148, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI203098753, Length=223, Percent_Identity=31.390134529148, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=32.5301204819277, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1786946, Length=224, Percent_Identity=36.1607142857143, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786305, Length=295, Percent_Identity=32.5423728813559, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17537937, Length=303, Percent_Identity=31.6831683168317, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=226, Percent_Identity=34.5132743362832, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17560088, Length=225, Percent_Identity=33.7777777777778, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17538894, Length=225, Percent_Identity=32, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=229, Percent_Identity=37.117903930131, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324258, Length=192, Percent_Identity=30.7291666666667, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI18859875, Length=307, Percent_Identity=30.9446254071661, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=35.2422907488987, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=29.2035398230088, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24582497, Length=226, Percent_Identity=29.2035398230088, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 32539; Mature: 32408

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKILATPKARAMAKHANIDLADLKIQHRKIEASDVENYIKSLKSAQTSAEKVEVSSSQQ
CCCCCCCCHHHHHHHHCCCCHHHEEEEHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCHH
AEKTSIPASIPPKLEGKREKIAPIRKAIARAMTNSNSVAYVNLVNQIDVSDLKLRKSVLD
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
PVLKTSGVKLTFLAFIAKAILIALSEFPVMAAKYDEATSEIVYPDTLNLGLAVDTDAGLM
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEECCCCCCCCEEEECCCCEE
VPVIKDAQKLSIVEIAKEIVRLAKAARERKIKPSEMQGGSFTITNYGSVGSLYGVPVINY
EHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
PELAIAGVGAIIDSAEVKDGQIVASKIMHLTVAADHRIDGATIGRFAARVKELLEKPEIL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
GIL
CCC
>Mature Secondary Structure 
SKILATPKARAMAKHANIDLADLKIQHRKIEASDVENYIKSLKSAQTSAEKVEVSSSQQ
CCCCCCCHHHHHHHHCCCCHHHEEEEHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCHH
AEKTSIPASIPPKLEGKREKIAPIRKAIARAMTNSNSVAYVNLVNQIDVSDLKLRKSVLD
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
PVLKTSGVKLTFLAFIAKAILIALSEFPVMAAKYDEATSEIVYPDTLNLGLAVDTDAGLM
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEECCCCCCCCEEEECCCCEE
VPVIKDAQKLSIVEIAKEIVRLAKAARERKIKPSEMQGGSFTITNYGSVGSLYGVPVINY
EHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
PELAIAGVGAIIDSAEVKDGQIVASKIMHLTVAADHRIDGATIGRFAARVKELLEKPEIL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
GIL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]