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The map label for this gene is pdhD

Identifier: 54020222

GI number: 54020222

Start: 635265

End: 637112

Strand: Direct

Name: pdhD

Synonym: mhp504

Alternate gene names: 54020222

Gene position: 635265-637112 (Clockwise)

Preceding gene: 54020221

Following gene: 54020223

Centisome position: 71.16

GC content: 36.2

Gene sequence:

>1848_bases
ATGTATAAATTTAAATTTGCTGATATCGGTGAGGGACTACATGAAGGTGTTGTTGCCCAAATTTATAAAAAAGAAGGGGA
AACAGTCAATGAAGGTGATTCGCTTTTTTCTGTTGAAACTGATAAAATAACAGCGGATATTCCTTCCCCAAAAACCGGAA
AAATCGTAAAAGTCTTAATGGCAGAAGGCGATACAATCCACGTCGGTCAAGAAATTTATCATATCGATGATGGCTCAAGA
GCTGAAACTGAAGTTGAAGAAGCAAAATCTGAAACCGAAGAAAAACCCGCTGGAGGAGCCAGTGTTGTGGGCGAAGTTAA
AGTTAGTGATGCACTTTTAAGCTTTGATTTTGGACCAAAAAAAGTAAAAACTACTTCACCAGAAAAAGTTTTAGTCCCTT
CAGATTCAAAAAAAGTTGAAGATAAAATTGAAAAAACTACAAATTCAGGTAAAATTTATCAGGGTCAGATTGAAAAAGAA
TTTGATGTAATTGTTGTGGGTTCTGGACCTGGAGGTTATCTTGCTGCTGCTGAAGCTGGAAATGCCGGACTTTCTACCTT
AATTGTTGAAAAAGAATTTTGAGGTGGAGTTTGTCTAAATGTTGGATGTATTCCGACAAAAGCAATGTTGAAAACCGCCG
AGGTTTTTGATTATCTTGAACAATTTAGTGATTTTGGACTAAGTGGAAATTCTGATCTTAAAATTTCCTGAGAAAAAATG
CACCAACGAAAAACCGAGGTGGTTAATAAATTAGTTGGGGGTGTTAAAGCAATTGTAAGATCGGCAAAAGCAACAAGTAT
TTTTGGTAAAGCTGAATTTGTCGGGGCACGTGAGATTAGTGTTGATGGTAAAGTTTATCGTGGAAAAAATGTGATTTTAG
CAACTGGTTCAGTTGATCGCAAATTAAATCTACCCGGTTTTGATAAGGGTTATAAATCTGGGAAAATTATTACCTCAAAA
GAAGCCATTAATTTAGAACAAAAAATTGATTCAATTGTAATAATTGGTGGTGGGGTAATTGGTGTTGAATTCGCCCAAAT
TTTTTCGGCTGCCGGAATTAAGGTAACAATTTTGCAAAATCTGCCAAGATTATTAGCAAATCTAGATAGTGAAATTTCGC
AGATAATTACTAAAAATTTAGTTGATAAAGGTGTTAAAGTTATTACAAATACCAACATTCTTCGTTTTGAAGATGATCAA
ATTATCTATGAATTTGAAGGTAAAACTGAGTTAATTACCGGTGAAAAAATTCTTGTAAGTATCGGTCGGCAAGCAAATTC
TCAAGGTCTTGCTGAGGTTGGAATTGAACTTGATAGTCGTGGTAGTGTTATAGTCGATGATCAATGCAGAACAAATGTTG
ATAGAGTTTATGCAATTGGCGACCTTTCGGCTAAGGCAATGCTAGCACATGTTGCCTATCGACATGCGGTTGTTTCAGTA
GCAGCAATTTTAGGTAAAACTGAAAAATATCAAGAAAAAACAGTACCTGCTTGCGTTTATACCCATCCAGAAATTGCTGT
TGTTGGTCTAACCGAAGAGCAAGCAAGGCAAGCTGGCTATGATTTTGTCATTGGAAAAGCAAGTTTTTCTCATATTGGGA
AAGCAATTGCCTCTGGAAACGCGTACGGATTTGCTAAATTAATCATTGATAAAAAATACGGGGAAATAATTGGTGCTCAC
ATTATCGGCCCGGTTGCAACTGATTTAATTTCAGAGATCGTGATCGCAATGGATAGTGAAGTAACAGTTTATGAACTTGC
AGCGGCAATCCACCCGCATCCAACTTATAGTGAAATTATTTGAGAAGCTGCGCGTTCTGCAGTTACAAAATTGAAGAAAA
TGAAATAA

Upstream 100 bases:

>100_bases
TTGCTGCAGATCATCGTTGAATTGATGGTGCAACAATTGGTCGTTTTGCTGCAAGAGTTAAAGAATTATTAGAAAAACCA
GAAATTTTAGGAATCTTATA

Downstream 100 bases:

>100_bases
ATTATAGAAAATTCAATTTGGTTTTAAATTCTTGAAAACGATGTAAAAAACTTTTTTGTTTTACATCGTTTTTTTTAAAT
TAATTTTAACGCATATTTTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MYKFKFADIGEGLHEGVVAQIYKKEGETVNEGDSLFSVETDKITADIPSPKTGKIVKVLMAEGDTIHVGQEIYHIDDGSR
AETEVEEAKSETEEKPAGGASVVGEVKVSDALLSFDFGPKKVKTTSPEKVLVPSDSKKVEDKIEKTTNSGKIYQGQIEKE
FDVIVVGSGPGGYLAAAEAGNAGLSTLIVEKEFWGGVCLNVGCIPTKAMLKTAEVFDYLEQFSDFGLSGNSDLKISWEKM
HQRKTEVVNKLVGGVKAIVRSAKATSIFGKAEFVGAREISVDGKVYRGKNVILATGSVDRKLNLPGFDKGYKSGKIITSK
EAINLEQKIDSIVIIGGGVIGVEFAQIFSAAGIKVTILQNLPRLLANLDSEISQIITKNLVDKGVKVITNTNILRFEDDQ
IIYEFEGKTELITGEKILVSIGRQANSQGLAEVGIELDSRGSVIVDDQCRTNVDRVYAIGDLSAKAMLAHVAYRHAVVSV
AAILGKTEKYQEKTVPACVYTHPEIAVVGLTEEQARQAGYDFVIGKASFSHIGKAIASGNAYGFAKLIIDKKYGEIIGAH
IIGPVATDLISEIVIAMDSEVTVYELAAAIHPHPTYSEIIWEAARSAVTKLKKMK

Sequences:

>Translated_615_residues
MYKFKFADIGEGLHEGVVAQIYKKEGETVNEGDSLFSVETDKITADIPSPKTGKIVKVLMAEGDTIHVGQEIYHIDDGSR
AETEVEEAKSETEEKPAGGASVVGEVKVSDALLSFDFGPKKVKTTSPEKVLVPSDSKKVEDKIEKTTNSGKIYQGQIEKE
FDVIVVGSGPGGYLAAAEAGNAGLSTLIVEKEF*GGVCLNVGCIPTKAMLKTAEVFDYLEQFSDFGLSGNSDLKIS*EKM
HQRKTEVVNKLVGGVKAIVRSAKATSIFGKAEFVGAREISVDGKVYRGKNVILATGSVDRKLNLPGFDKGYKSGKIITSK
EAINLEQKIDSIVIIGGGVIGVEFAQIFSAAGIKVTILQNLPRLLANLDSEISQIITKNLVDKGVKVITNTNILRFEDDQ
IIYEFEGKTELITGEKILVSIGRQANSQGLAEVGIELDSRGSVIVDDQCRTNVDRVYAIGDLSAKAMLAHVAYRHAVVSV
AAILGKTEKYQEKTVPACVYTHPEIAVVGLTEEQARQAGYDFVIGKASFSHIGKAIASGNAYGFAKLIIDKKYGEIIGAH
IIGPVATDLISEIVIAMDSEVTVYELAAAIHPHPTYSEII*EAARSAVTKLKKMK
>Mature_615_residues
MYKFKFADIGEGLHEGVVAQIYKKEGETVNEGDSLFSVETDKITADIPSPKTGKIVKVLMAEGDTIHVGQEIYHIDDGSR
AETEVEEAKSETEEKPAGGASVVGEVKVSDALLSFDFGPKKVKTTSPEKVLVPSDSKKVEDKIEKTTNSGKIYQGQIEKE
FDVIVVGSGPGGYLAAAEAGNAGLSTLIVEKEF*GGVCLNVGCIPTKAMLKTAEVFDYLEQFSDFGLSGNSDLKIS*EKM
HQRKTEVVNKLVGGVKAIVRSAKATSIFGKAEFVGAREISVDGKVYRGKNVILATGSVDRKLNLPGFDKGYKSGKIITSK
EAINLEQKIDSIVIIGGGVIGVEFAQIFSAAGIKVTILQNLPRLLANLDSEISQIITKNLVDKGVKVITNTNILRFEDDQ
IIYEFEGKTELITGEKILVSIGRQANSQGLAEVGIELDSRGSVIVDDQCRTNVDRVYAIGDLSAKAMLAHVAYRHAVVSV
AAILGKTEKYQEKTVPACVYTHPEIAVVGLTEEQARQAGYDFVIGKASFSHIGKAIASGNAYGFAKLIIDKKYGEIIGAH
IIGPVATDLISEIVIAMDSEVTVYELAAAIHPHPTYSEII*EAARSAVTKLKKMK

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=465, Percent_Identity=33.9784946236559, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=27.9220779220779, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI148277071, Length=431, Percent_Identity=26.4501160092807, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI148277065, Length=431, Percent_Identity=26.4501160092807, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI33519430, Length=431, Percent_Identity=26.4501160092807, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI33519428, Length=431, Percent_Identity=26.4501160092807, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI33519426, Length=431, Percent_Identity=26.4501160092807, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI291045266, Length=431, Percent_Identity=27.3781902552204, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI22035672, Length=429, Percent_Identity=24.009324009324, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI291045268, Length=427, Percent_Identity=25.0585480093677, Blast_Score=97, Evalue=6e-20,
Organism=Escherichia coli, GI1786307, Length=446, Percent_Identity=33.8565022421525, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87081717, Length=450, Percent_Identity=27.3333333333333, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI87082354, Length=424, Percent_Identity=27.8301886792453, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789915, Length=421, Percent_Identity=26.1282660332542, Blast_Score=140, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=34.7732181425486, Blast_Score=258, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI17557007, Length=472, Percent_Identity=26.4830508474576, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=468, Percent_Identity=26.4957264957265, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=468, Percent_Identity=26.4957264957265, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=437, Percent_Identity=25.4004576659039, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=197, Percent_Identity=30.4568527918782, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=33.8266384778013, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=27.2921108742004, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=25.4777070063694, Blast_Score=147, Evalue=5e-36,
Organism=Drosophila melanogaster, GI21358499, Length=464, Percent_Identity=35.1293103448276, Blast_Score=244, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24640549, Length=484, Percent_Identity=26.4462809917355, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640553, Length=485, Percent_Identity=26.3917525773196, Blast_Score=120, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24640551, Length=485, Percent_Identity=26.3917525773196, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17737741, Length=513, Percent_Identity=23.5867446393762, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 65653; Mature: 65653

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKFKFADIGEGLHEGVVAQIYKKEGETVNEGDSLFSVETDKITADIPSPKTGKIVKVLM
CCEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECCCCCCCCEEEEEE
AEGDTIHVGQEIYHIDDGSRAETEVEEAKSETEEKPAGGASVVGEVKVSDALLSFDFGPK
ECCCEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECEEEECCCCCC
KVKTTSPEKVLVPSDSKKVEDKIEKTTNSGKIYQGQIEKEFDVIVVGSGPGGYLAAAEAG
CCCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCCCEEEEECCC
NAGLSTLIVEKEFGGVCLNVGCIPTKAMLKTAEVFDYLEQFSDFGLSGNSDLKISEKMHQ
CCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
RKTEVVNKLVGGVKAIVRSAKATSIFGKAEFVGAREISVDGKVYRGKNVILATGSVDRKL
HHHHHHHHHHHHHHHHHHHHHHHEEECCHHCCCCEEEECCCEEEECCEEEEEECCCCCEE
NLPGFDKGYKSGKIITSKEAINLEQKIDSIVIIGGGVIGVEFAQIFSAAGIKVTILQNLP
CCCCCCCCCCCCCEEECHHHCCHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEEHHHHHH
RLLANLDSEISQIITKNLVDKGVKVITNTNILRFEDDQIIYEFEGKTELITGEKILVSIG
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCEEEEEECCCEEEEECCEEEEEEC
RQANSQGLAEVGIELDSRGSVIVDDQCRTNVDRVYAIGDLSAKAMLAHVAYRHAVVSVAA
CCCCCCCHHHCCEEECCCCCEEECCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
ILGKTEKYQEKTVPACVYTHPEIAVVGLTEEQARQAGYDFVIGKASFSHIGKAIASGNAY
HHCCCHHHHHCCCCEEEEECCCEEEEECCHHHHHHCCCCEEEECCCHHHHHHHHHCCCCC
GFAKLIIDKKYGEIIGAHIIGPVATDLISEIVIAMDSEVTVYELAAAIHPHPTYSEIIEA
EEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHH
ARSAVTKLKKMK
HHHHHHHHHCCC
>Mature Secondary Structure
MYKFKFADIGEGLHEGVVAQIYKKEGETVNEGDSLFSVETDKITADIPSPKTGKIVKVLM
CCEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECCCCCCCCEEEEEE
AEGDTIHVGQEIYHIDDGSRAETEVEEAKSETEEKPAGGASVVGEVKVSDALLSFDFGPK
ECCCEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECEEEECCCCCC
KVKTTSPEKVLVPSDSKKVEDKIEKTTNSGKIYQGQIEKEFDVIVVGSGPGGYLAAAEAG
CCCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCCCEEEEECCC
NAGLSTLIVEKEFGGVCLNVGCIPTKAMLKTAEVFDYLEQFSDFGLSGNSDLKISEKMHQ
CCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
RKTEVVNKLVGGVKAIVRSAKATSIFGKAEFVGAREISVDGKVYRGKNVILATGSVDRKL
HHHHHHHHHHHHHHHHHHHHHHHEEECCHHCCCCEEEECCCEEEECCEEEEEECCCCCEE
NLPGFDKGYKSGKIITSKEAINLEQKIDSIVIIGGGVIGVEFAQIFSAAGIKVTILQNLP
CCCCCCCCCCCCCEEECHHHCCHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEEHHHHHH
RLLANLDSEISQIITKNLVDKGVKVITNTNILRFEDDQIIYEFEGKTELITGEKILVSIG
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCEEEEEECCCEEEEECCEEEEEEC
RQANSQGLAEVGIELDSRGSVIVDDQCRTNVDRVYAIGDLSAKAMLAHVAYRHAVVSVAA
CCCCCCCHHHCCEEECCCCCEEECCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
ILGKTEKYQEKTVPACVYTHPEIAVVGLTEEQARQAGYDFVIGKASFSHIGKAIASGNAY
HHCCCHHHHHCCCCEEEEECCCEEEEECCHHHHHHCCCCEEEECCCHHHHHHHHHCCCCC
GFAKLIIDKKYGEIIGAHIIGPVATDLISEIVIAMDSEVTVYELAAAIHPHPTYSEIIEA
EEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHH
ARSAVTKLKKMK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]