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The map label for this gene is nadE

Identifier: 54020213

GI number: 54020213

Start: 584587

End: 585327

Strand: Direct

Name: nadE

Synonym: mhp471

Alternate gene names: 54020213

Gene position: 584587-585327 (Clockwise)

Preceding gene: 54020212

Following gene: 54020214

Centisome position: 65.48

GC content: 29.69

Gene sequence:

>741_bases
ATGATAAATAAGCAAGATTTAATAAATTATTTTAACTATCTTATTGAGTGGATTAGGCAAGAAGTTAAAAAAGCCCAGAA
AAAAGGTGTTATTTTTGGACTATCGGGAGGGGTTGATTCAGCTTTAGTTGCTGTTTTAGCAAATAAAGCTTTTCCAGATT
CACACTTAGGCTTGATAATGCCGATTCGCGATATGATAACTGATATGGTTGATATTGATCTTTTAGTTAAAAAATTTGGT
ATTAAAAATAAAGAAATTAATCTAAAACCAGCCTTTGAAAATTTGAAAAAATGCTTTAATTTAAAAAATAAATTAGCAAA
TTCAAATATCCAACCACGCCTTAGAATGACTAGTTTGTATGCATTTGCCCAAGAATTTGATTATTTAGTGCTTGGGACAG
ATAATTTTTCGGAAATGTATCTAGGATATTTTACAAAATATGGCGATGGCGGGGTCGATTTATTACCAATCGTTAATTTA
ACTAAAGTTCAAGTTTGAAAAATGGCAGCTGAAATTGGGATTCCAGAATCAATAATTAAGAAAAAACCAAGTGCAAATCT
TTGAGAAAATCAAACTGATGAAAACGAAATGGGATTCAGCTATGGTGATTTAGATCTTTTTATGGAAAACCCAAATCTTG
TAAGTAAGGAAATTGCTGCAAAAATTACAAAATTACATGAAATAAGTAGTCATAAAAGGGATCAAATTCCTCGTCCTTTA
AAAAAATTAGGAGAAATTTAA

Upstream 100 bases:

>100_bases
AATAATATAATTTTTCGCTTTAGCTCTAGAATATAATTTTTTTTAATTCGTATTTTTTTCTTTGGAAAAAAATAAGTTTT
TGATATACTTTTAAAAGTTT

Downstream 100 bases:

>100_bases
TGGCTGGACATTCAAAATGAGCAAACATAAAACACCGAAAAGGTGCTCAAGATGCCCTAAAAGCAAAAATTTTTAACAAA
TTTTCCAAAGAAATTATGGT

Product: NH(3)-dependent NAD+ synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MINKQDLINYFNYLIEWIRQEVKKAQKKGVIFGLSGGVDSALVAVLANKAFPDSHLGLIMPIRDMITDMVDIDLLVKKFG
IKNKEINLKPAFENLKKCFNLKNKLANSNIQPRLRMTSLYAFAQEFDYLVLGTDNFSEMYLGYFTKYGDGGVDLLPIVNL
TKVQVWKMAAEIGIPESIIKKKPSANLWENQTDENEMGFSYGDLDLFMENPNLVSKEIAAKITKLHEISSHKRDQIPRPL
KKLGEI

Sequences:

>Translated_246_residues
MINKQDLINYFNYLIEWIRQEVKKAQKKGVIFGLSGGVDSALVAVLANKAFPDSHLGLIMPIRDMITDMVDIDLLVKKFG
IKNKEINLKPAFENLKKCFNLKNKLANSNIQPRLRMTSLYAFAQEFDYLVLGTDNFSEMYLGYFTKYGDGGVDLLPIVNL
TKVQV*KMAAEIGIPESIIKKKPSANL*ENQTDENEMGFSYGDLDLFMENPNLVSKEIAAKITKLHEISSHKRDQIPRPL
KKLGEI
>Mature_246_residues
MINKQDLINYFNYLIEWIRQEVKKAQKKGVIFGLSGGVDSALVAVLANKAFPDSHLGLIMPIRDMITDMVDIDLLVKKFG
IKNKEINLKPAFENLKKCFNLKNKLANSNIQPRLRMTSLYAFAQEFDYLVLGTDNFSEMYLGYFTKYGDGGVDLLPIVNL
TKVQV*KMAAEIGIPESIIKKKPSANL*ENQTDENEMGFSYGDLDLFMENPNLVSKEIAAKITKLHEISSHKRDQIPRPL
KKLGEI

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=225, Percent_Identity=29.3333333333333, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24641841, Length=160, Percent_Identity=33.125, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641843, Length=160, Percent_Identity=33.125, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 27597; Mature: 27597

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKQDLINYFNYLIEWIRQEVKKAQKKGVIFGLSGGVDSALVAVLANKAFPDSHLGLIM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHC
PIRDMITDMVDIDLLVKKFGIKNKEINLKPAFENLKKCFNLKNKLANSNIQPRLRMTSLY
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AFAQEFDYLVLGTDNFSEMYLGYFTKYGDGGVDLLPIVNLTKVQVKMAAEIGIPESIIKK
HHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHCC
KPSANLENQTDENEMGFSYGDLDLFMENPNLVSKEIAAKITKLHEISSHKRDQIPRPLKK
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LGEI
HCCC
>Mature Secondary Structure
MINKQDLINYFNYLIEWIRQEVKKAQKKGVIFGLSGGVDSALVAVLANKAFPDSHLGLIM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHC
PIRDMITDMVDIDLLVKKFGIKNKEINLKPAFENLKKCFNLKNKLANSNIQPRLRMTSLY
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AFAQEFDYLVLGTDNFSEMYLGYFTKYGDGGVDLLPIVNLTKVQVKMAAEIGIPESIIKK
HHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHCC
KPSANLENQTDENEMGFSYGDLDLFMENPNLVSKEIAAKITKLHEISSHKRDQIPRPLKK
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LGEI
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]