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The map label for this gene is ptsI

Identifier: 54020212

GI number: 54020212

Start: 582677

End: 584413

Strand: Direct

Name: ptsI

Synonym: mhp470

Alternate gene names: 54020212

Gene position: 582677-584413 (Clockwise)

Preceding gene: 54020211

Following gene: 54020213

Centisome position: 65.27

GC content: 32.07

Gene sequence:

>1737_bases
ATGGCAAAACTTAATTCTTATAAATTTAAAGGAATCGGGGCCTCAAGCGGAATTGCAATCGCAAAAGTTTTTAAATTAGT
TGAACAAAAAATTGAAATTAGTGATCAAAAAGTTGAGGATATTAGCACTGAAATTCAAATTTTTGAAGAGGGGGTAAAAA
AATCTGTTAGCCAAATTGAAAAAATTAAAAAATTAGCAACTAAGTTAGCAAAAGATGAGCTCGAAATTCTTGATGCTCAT
ATTTTGATTGCAACAGATCCTGTTCTTAAAGAAGATACAATCAATTTAATCAAGCAAGGATATTCAGCTGCTTATTCTTT
AAAAGAAACGGCAAAAAATCATACTAATTTTTTACTTGAAACCGGGGATGAATATTTAATGGCTCGAGCAACTGATATCA
AAGATGCCTCACAAAGAATTATTAAAAATATTTTAAATTTGAATATTTTAGATTTAAGTGCAATCGATAAGGATGTAATA
ATTGTTGCTGATGATCTAAAGCCCTCAGATACCGCCCAACTAAATTCATATGTTAAAGGTTTTGCAACTAATATTGGCTC
AAAAACTTCGCATTCAGCAATCATGGCGCGAAGTTTAGAAATTCCAGCAATTTTAGGTGTTAGTGATATTCTTGAAAAAG
TTGAAAATGATGATATTTTAGCAATTAATGGCGATCTTGGCTATGGAATTATTAACCCAAATCAAGAAGAAATTGAAGAA
TTTGAGCGCCAAAAAGCCAATTTTGAAGCTGAAAAAGAAAAATTACAAGAATTTTTAAATAAAAAAGCAACTAGTGCTGA
TGGAAAAAAGGTTGTAATTGCTGCTAATATTGGATCAGTTGAAGATTCATATGCAGCAAAAAAAGTAAATGCTGATGAAA
TTGGGCTTTTTCGCTCAGAATTTTTATATATGGACAGGTCAAATTGGCCTTCTGAAGAAGAGCAATTTTTAAGTTATAAA
GCCGTAATCGAGAGTCAAAATCCGAAAAAAGTTGTTGTTAGAACCCTTGATATCGGCGGCGATAAAACTTTAAAATACTT
TGATTTTGCTAAGGAATTAAACCCTTTTTTAGGCTATCGGGCAATTCGTCTTTGTCTTGATAAATTAGAAATTTTTAAAA
CTCAGCTTCGGGCACTAGTAAGGGCTTCTGAATTTGGAAATTTAGCAATTATGTTTCCCATGGTTGCAAGTCTTGATGAA
TTTTTTCAGGCAAGAGCGGTTTTTGATGAGGTATATAATCAAATTAAGGCTAAATATCCAAATGTTGCTCCAAAAAGCAA
CATTAAAATCGGAATTATGATTGAAATTCCGATTTCTGCAATTCTTGCTGATAAGTTTGCCGAACATGTTGATTTTTTCT
CTATTGGCACAAATGATTTAATTCAATACTCCTTTGCCGCTGATCGAATGAATGAAAAAGTCGCTTATTTATATCAAACT
TTAAACCCCGGTCTGTTAAAACTTATTAAAATGACGATTGATGCCGCCCATGCGAAAGGAAAATGAGTGGGAATGTGTGG
CGAAATGGCAGGGGATATCAATGCTGTACCGCTTTTATTAGGTCTTGGACTTGATGAATTTTCAGTTTCTGCAAGTAGTG
TTTTAAAAGTTAAGAAACTAATTTCCGAACTAAATTATCAAAAAATGCAGGAAATAGCAAAAAAAACGCTTGAATTTTCA
ACTGAAGAGCAAGTAATAAAATATCTTGAGTCTTTAAATTTAATTCGCCATAACTAA

Upstream 100 bases:

>100_bases
TTATTGACAAATATTTATTAAAATAAGATAAAAATTTTATTATTTTTTGATATAATTTTTTTTAGTAAATAGTTTTTGTG
TTTAAAACTGGAAGGAAAAG

Downstream 100 bases:

>100_bases
AAAAGCACTTAAATTTAAAGACTTTTTTATATTAAATACAAAAAAATATCAAAAACCGAAAAATTTAAAAAAAAATAATA
TAATTTTTCGCTTTAGCTCT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MAKLNSYKFKGIGASSGIAIAKVFKLVEQKIEISDQKVEDISTEIQIFEEGVKKSVSQIEKIKKLATKLAKDELEILDAH
ILIATDPVLKEDTINLIKQGYSAAYSLKETAKNHTNFLLETGDEYLMARATDIKDASQRIIKNILNLNILDLSAIDKDVI
IVADDLKPSDTAQLNSYVKGFATNIGSKTSHSAIMARSLEIPAILGVSDILEKVENDDILAINGDLGYGIINPNQEEIEE
FERQKANFEAEKEKLQEFLNKKATSADGKKVVIAANIGSVEDSYAAKKVNADEIGLFRSEFLYMDRSNWPSEEEQFLSYK
AVIESQNPKKVVVRTLDIGGDKTLKYFDFAKELNPFLGYRAIRLCLDKLEIFKTQLRALVRASEFGNLAIMFPMVASLDE
FFQARAVFDEVYNQIKAKYPNVAPKSNIKIGIMIEIPISAILADKFAEHVDFFSIGTNDLIQYSFAADRMNEKVAYLYQT
LNPGLLKLIKMTIDAAHAKGKWVGMCGEMAGDINAVPLLLGLGLDEFSVSASSVLKVKKLISELNYQKMQEIAKKTLEFS
TEEQVIKYLESLNLIRHN

Sequences:

>Translated_578_residues
MAKLNSYKFKGIGASSGIAIAKVFKLVEQKIEISDQKVEDISTEIQIFEEGVKKSVSQIEKIKKLATKLAKDELEILDAH
ILIATDPVLKEDTINLIKQGYSAAYSLKETAKNHTNFLLETGDEYLMARATDIKDASQRIIKNILNLNILDLSAIDKDVI
IVADDLKPSDTAQLNSYVKGFATNIGSKTSHSAIMARSLEIPAILGVSDILEKVENDDILAINGDLGYGIINPNQEEIEE
FERQKANFEAEKEKLQEFLNKKATSADGKKVVIAANIGSVEDSYAAKKVNADEIGLFRSEFLYMDRSNWPSEEEQFLSYK
AVIESQNPKKVVVRTLDIGGDKTLKYFDFAKELNPFLGYRAIRLCLDKLEIFKTQLRALVRASEFGNLAIMFPMVASLDE
FFQARAVFDEVYNQIKAKYPNVAPKSNIKIGIMIEIPISAILADKFAEHVDFFSIGTNDLIQYSFAADRMNEKVAYLYQT
LNPGLLKLIKMTIDAAHAKGK*VGMCGEMAGDINAVPLLLGLGLDEFSVSASSVLKVKKLISELNYQKMQEIAKKTLEFS
TEEQVIKYLESLNLIRHN
>Mature_577_residues
AKLNSYKFKGIGASSGIAIAKVFKLVEQKIEISDQKVEDISTEIQIFEEGVKKSVSQIEKIKKLATKLAKDELEILDAHI
LIATDPVLKEDTINLIKQGYSAAYSLKETAKNHTNFLLETGDEYLMARATDIKDASQRIIKNILNLNILDLSAIDKDVII
VADDLKPSDTAQLNSYVKGFATNIGSKTSHSAIMARSLEIPAILGVSDILEKVENDDILAINGDLGYGIINPNQEEIEEF
ERQKANFEAEKEKLQEFLNKKATSADGKKVVIAANIGSVEDSYAAKKVNADEIGLFRSEFLYMDRSNWPSEEEQFLSYKA
VIESQNPKKVVVRTLDIGGDKTLKYFDFAKELNPFLGYRAIRLCLDKLEIFKTQLRALVRASEFGNLAIMFPMVASLDEF
FQARAVFDEVYNQIKAKYPNVAPKSNIKIGIMIEIPISAILADKFAEHVDFFSIGTNDLIQYSFAADRMNEKVAYLYQTL
NPGLLKLIKMTIDAAHAKGK*VGMCGEMAGDINAVPLLLGLGLDEFSVSASSVLKVKKLISELNYQKMQEIAKKTLEFST
EEQVIKYLESLNLIRHN

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=561, Percent_Identity=44.7415329768271, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI48994992, Length=507, Percent_Identity=34.5167652859961, Blast_Score=303, Evalue=3e-83,
Organism=Escherichia coli, GI1789193, Length=576, Percent_Identity=32.1180555555556, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1788726, Length=448, Percent_Identity=35.0446428571429, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1787994, Length=232, Percent_Identity=33.1896551724138, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64343; Mature: 64212

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLNSYKFKGIGASSGIAIAKVFKLVEQKIEISDQKVEDISTEIQIFEEGVKKSVSQIE
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KIKKLATKLAKDELEILDAHILIATDPVLKEDTINLIKQGYSAAYSLKETAKNHTNFLLE
HHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TGDEYLMARATDIKDASQRIIKNILNLNILDLSAIDKDVIIVADDLKPSDTAQLNSYVKG
CCCHHHHEECCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHH
FATNIGSKTSHSAIMARSLEIPAILGVSDILEKVENDDILAINGDLGYGIINPNQEEIEE
HHHHCCCCCCHHHHHHHCCCCCHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHH
FERQKANFEAEKEKLQEFLNKKATSADGKKVVIAANIGSVEDSYAAKKVNADEIGLFRSE
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHH
FLYMDRSNWPSEEEQFLSYKAVIESQNPKKVVVRTLDIGGDKTLKYFDFAKELNPFLGYR
HEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCHHHHHH
AIRLCLDKLEIFKTQLRALVRASEFGNLAIMFPMVASLDEFFQARAVFDEVYNQIKAKYP
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NVAPKSNIKIGIMIEIPISAILADKFAEHVDFFSIGTNDLIQYSFAADRMNEKVAYLYQT
CCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LNPGLLKLIKMTIDAAHAKGKVGMCGEMAGDINAVPLLLGLGLDEFSVSASSVLKVKKLI
CCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHCCCCHHHCCHHHHHHHHHHH
SELNYQKMQEIAKKTLEFSTEEQVIKYLESLNLIRHN
HHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKLNSYKFKGIGASSGIAIAKVFKLVEQKIEISDQKVEDISTEIQIFEEGVKKSVSQIE
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KIKKLATKLAKDELEILDAHILIATDPVLKEDTINLIKQGYSAAYSLKETAKNHTNFLLE
HHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TGDEYLMARATDIKDASQRIIKNILNLNILDLSAIDKDVIIVADDLKPSDTAQLNSYVKG
CCCHHHHEECCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHH
FATNIGSKTSHSAIMARSLEIPAILGVSDILEKVENDDILAINGDLGYGIINPNQEEIEE
HHHHCCCCCCHHHHHHHCCCCCHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHH
FERQKANFEAEKEKLQEFLNKKATSADGKKVVIAANIGSVEDSYAAKKVNADEIGLFRSE
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHH
FLYMDRSNWPSEEEQFLSYKAVIESQNPKKVVVRTLDIGGDKTLKYFDFAKELNPFLGYR
HEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCHHHHHH
AIRLCLDKLEIFKTQLRALVRASEFGNLAIMFPMVASLDEFFQARAVFDEVYNQIKAKYP
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NVAPKSNIKIGIMIEIPISAILADKFAEHVDFFSIGTNDLIQYSFAADRMNEKVAYLYQT
CCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LNPGLLKLIKMTIDAAHAKGKVGMCGEMAGDINAVPLLLGLGLDEFSVSASSVLKVKKLI
CCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHCCCCHHHCCHHHHHHHHHHH
SELNYQKMQEIAKKTLEFSTEEQVIKYLESLNLIRHN
HHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1551842; 1901791 [H]