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The map label for this gene is tktA

Identifier: 54020200

GI number: 54020200

Start: 508289

End: 510136

Strand: Direct

Name: tktA

Synonym: mhp431

Alternate gene names: 54020200

Gene position: 508289-510136 (Clockwise)

Preceding gene: 54020199

Following gene: 54020201

Centisome position: 56.93

GC content: 34.58

Gene sequence:

>1848_bases
ATGCTCAAAAATAACATTGAAAATTTAGCAATAAATTCTTTGAAAATGCATGGTGTGGCTGCGGTTAATAAAGCAAATTC
AGGTCATCCTGGAGTGATTTTAAGTGCTGCGAAAATGGTTTATGCATTATATCGCGATCATATGAATTTTGATGTTGAAA
ATCTAAATTGAATAAATCGCGATCGGTTTATTTTATCTGCCGGACATGCTTCTGGACTTTTATATTCTTTATTATATAAT
TTAGGAATTCTAGAGCAAAAAGATCTTGAAAATTTTCGCCAAATGGGATCAAAAACTCCCGGACATCCAGAATATGGACA
TACTTTAGGAGTTGAGGCAACAACAGGACCACTTGGTCAAGGAATCGCAATGGGTGTGGGCCTTGCGATGGCGCAGGCAC
ATTTGCATTCTAAATTTCCCGAAATTAATCATTATACTTATATTTTATGCGGCGATGGCGATCTCCAAGAGGGAATATCT
TATGAATCACTTTCGTTAGCCGGTCATCTGAAACTAGAAAATCTTATTATCCTTTATGATTCAAATGATATCCAACTTGA
TTCCGCTCTTGATGTGGTTTTTAGTGAAAATGTCCGTCAAAGAATTGAATCACAAGGTCTTTTTTACCAATTAGTTGAAA
AAAATGATGTTAATTTAATCTCAGAAGCGATTGCTAAGGCAAAAAAATCAAAAAAACCGAGTTTTATCGAGATTAAAACA
ATTATCGGCGAAGGTACAAGCAAAGAAAAAACAAGCGATGTCCATGGTGCTCCACTTGGAAATGATATTCTTACTTTGAA
AAAAAATCTTAATTGAGAATATGATGATTTTTATCTTGCAGATGAACTAAGACAACACTGAAAAAATACCCTTGGAAAAC
GAAGTCAAAAAGCATTTGCTGAATTTAAAATTTCAAAAGAACTTAAAGAATTTCTTGAAAAAGGGCAAAATCTTGATCTT
GAAATTGATTTAGATCTGGCAAAAAATCAGGCAACTCGTAATACTTCAGGCCAAATTCTTGATTATATTTCCAAACATCT
TCCTTACTGAATCGGCGGTTCGGCTGATTTGTCAGTTTCAACGAAGGCCAAAGGGAGTGATGGAATTTTTAGTTGCCAAA
ATTATCAGGGAAGAAACATTATGTTTGGTGTCCGCGAGTTTGCAATGGCCTCAATTGCTAACGGGATTGCTTTACATTCA
GTTTTACGTCCTTTTGTTTCAACTTTTTTTGTTTTTGCGGATTATCTAAAACCAGCTTTGCGACTTTCAGCAATTATGAA
ATTGCCAGTTAGCTACATTTTCAGTCATGATTCCTTAATGGTAGGGGAAGATGGACCGACTCATGAACCAGTTGAACAAC
TCGCAATGTTAAGATCATTACCAAATCTTGCAGTTTATCGACCTGGCGATGAAAACGAATTAAAAGCGGCCTATGAATTA
ATTTTAGCAAAAAAAAATCAGCCTTCGGCTCTAATTCTGACAAGGCAAAACATTAAATCCTTTAATTCCTGCAAAAAAGG
CTTTAAAAAAGGCGCTTATTTAGTAAGAAAAGCATCTTCAAAATGAGCCCTGATTGCTACCGGTTCTGAGCTCGGTCTTG
CCAATGAAATTGCTAATAAATTAGATGTTAATCTAATTTCTTTATCAAATTGGCAGAATGGTCCAATTTGAGATCCAAAT
TATGCAATTTCACTTGAATTAGCCACAACTTTTGGCTGAAAAGCGCATGCAAAATATAATTTTGGCCATGATGATTTCGG
ATTTTCAGCCCCAGCTGACAAAATTTTAGAAAAAATTGGCTTTAATTTTGATAATTTAGTTACAAAAATAAAGAAAATTA
TAGTTTAG

Upstream 100 bases:

>100_bases
GAAGGCGAGAGTGTAATTGTATCAACAGAAGATGGGAAGTATGTCTCGCGAGCGTAAAATAAAAACGAAAGATAAAAAAA
TAAAAAGGGAAAAAATTAAA

Downstream 100 bases:

>100_bases
ATTAAAATTTTCTTTATTTTTGTAAAAAAGCATTAATACAAAATAAAAAAGTAAAAATTCTCACTTTTTTATTTTGTATT
TTTCTTATTTCAATTAAAAA

Product: transketolase

Products: NA

Alternate protein names: TK [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MLKNNIENLAINSLKMHGVAAVNKANSGHPGVILSAAKMVYALYRDHMNFDVENLNWINRDRFILSAGHASGLLYSLLYN
LGILEQKDLENFRQMGSKTPGHPEYGHTLGVEATTGPLGQGIAMGVGLAMAQAHLHSKFPEINHYTYILCGDGDLQEGIS
YESLSLAGHLKLENLIILYDSNDIQLDSALDVVFSENVRQRIESQGLFYQLVEKNDVNLISEAIAKAKKSKKPSFIEIKT
IIGEGTSKEKTSDVHGAPLGNDILTLKKNLNWEYDDFYLADELRQHWKNTLGKRSQKAFAEFKISKELKEFLEKGQNLDL
EIDLDLAKNQATRNTSGQILDYISKHLPYWIGGSADLSVSTKAKGSDGIFSCQNYQGRNIMFGVREFAMASIANGIALHS
VLRPFVSTFFVFADYLKPALRLSAIMKLPVSYIFSHDSLMVGEDGPTHEPVEQLAMLRSLPNLAVYRPGDENELKAAYEL
ILAKKNQPSALILTRQNIKSFNSCKKGFKKGAYLVRKASSKWALIATGSELGLANEIANKLDVNLISLSNWQNGPIWDPN
YAISLELATTFGWKAHAKYNFGHDDFGFSAPADKILEKIGFNFDNLVTKIKKIIV

Sequences:

>Translated_615_residues
MLKNNIENLAINSLKMHGVAAVNKANSGHPGVILSAAKMVYALYRDHMNFDVENLN*INRDRFILSAGHASGLLYSLLYN
LGILEQKDLENFRQMGSKTPGHPEYGHTLGVEATTGPLGQGIAMGVGLAMAQAHLHSKFPEINHYTYILCGDGDLQEGIS
YESLSLAGHLKLENLIILYDSNDIQLDSALDVVFSENVRQRIESQGLFYQLVEKNDVNLISEAIAKAKKSKKPSFIEIKT
IIGEGTSKEKTSDVHGAPLGNDILTLKKNLN*EYDDFYLADELRQH*KNTLGKRSQKAFAEFKISKELKEFLEKGQNLDL
EIDLDLAKNQATRNTSGQILDYISKHLPY*IGGSADLSVSTKAKGSDGIFSCQNYQGRNIMFGVREFAMASIANGIALHS
VLRPFVSTFFVFADYLKPALRLSAIMKLPVSYIFSHDSLMVGEDGPTHEPVEQLAMLRSLPNLAVYRPGDENELKAAYEL
ILAKKNQPSALILTRQNIKSFNSCKKGFKKGAYLVRKASSK*ALIATGSELGLANEIANKLDVNLISLSNWQNGPI*DPN
YAISLELATTFG*KAHAKYNFGHDDFGFSAPADKILEKIGFNFDNLVTKIKKIIV
>Mature_615_residues
MLKNNIENLAINSLKMHGVAAVNKANSGHPGVILSAAKMVYALYRDHMNFDVENLN*INRDRFILSAGHASGLLYSLLYN
LGILEQKDLENFRQMGSKTPGHPEYGHTLGVEATTGPLGQGIAMGVGLAMAQAHLHSKFPEINHYTYILCGDGDLQEGIS
YESLSLAGHLKLENLIILYDSNDIQLDSALDVVFSENVRQRIESQGLFYQLVEKNDVNLISEAIAKAKKSKKPSFIEIKT
IIGEGTSKEKTSDVHGAPLGNDILTLKKNLN*EYDDFYLADELRQH*KNTLGKRSQKAFAEFKISKELKEFLEKGQNLDL
EIDLDLAKNQATRNTSGQILDYISKHLPY*IGGSADLSVSTKAKGSDGIFSCQNYQGRNIMFGVREFAMASIANGIALHS
VLRPFVSTFFVFADYLKPALRLSAIMKLPVSYIFSHDSLMVGEDGPTHEPVEQLAMLRSLPNLAVYRPGDENELKAAYEL
ILAKKNQPSALILTRQNIKSFNSCKKGFKKGAYLVRKASSK*ALIATGSELGLANEIANKLDVNLISLSNWQNGPI*DPN
YAISLELATTFG*KAHAKYNFGHDDFGFSAPADKILEKIGFNFDNLVTKIKKIIV

Specific function: Unknown

COG id: COG0021

COG function: function code G; Transketolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI205277463, Length=560, Percent_Identity=27.5, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI4507521, Length=560, Percent_Identity=27.5, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI133778974, Length=568, Percent_Identity=27.2887323943662, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI225637459, Length=566, Percent_Identity=22.791519434629, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI225637463, Length=467, Percent_Identity=23.982869379015, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI225637461, Length=467, Percent_Identity=23.982869379015, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI48994911, Length=661, Percent_Identity=37.0650529500756, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1788808, Length=670, Percent_Identity=36.5671641791045, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17539652, Length=480, Percent_Identity=26.6666666666667, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6325331, Length=667, Percent_Identity=39.4302848575712, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6319593, Length=669, Percent_Identity=37.8176382660688, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI45551847, Length=560, Percent_Identity=28.2142857142857, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI45550715, Length=560, Percent_Identity=28.2142857142857, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24645119, Length=504, Percent_Identity=28.968253968254, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24666278, Length=481, Percent_Identity=27.4428274428274, Blast_Score=155, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005478
- InterPro:   IPR020826
- InterPro:   IPR005476
- InterPro:   IPR005474 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C; PF00456 Transketolase_N [H]

EC number: =2.2.1.1 [H]

Molecular weight: Translated: 66939; Mature: 66939

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00802 TRANSKETOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNNIENLAINSLKMHGVAAVNKANSGHPGVILSAAKMVYALYRDHMNFDVENLNINRD
CCCCCCCHHHHHHEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCC
RFILSAGHASGLLYSLLYNLGILEQKDLENFRQMGSKTPGHPEYGHTLGVEATTGPLGQG
EEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCH
IAMGVGLAMAQAHLHSKFPEINHYTYILCGDGDLQEGISYESLSLAGHLKLENLIILYDS
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCCCCCEEEEEEEEEEEEEEEECC
NDIQLDSALDVVFSENVRQRIESQGLFYQLVEKNDVNLISEAIAKAKKSKKPSFIEIKTI
CCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEE
IGEGTSKEKTSDVHGAPLGNDILTLKKNLNEYDDFYLADELRQHKNTLGKRSQKAFAEFK
ECCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISKELKEFLEKGQNLDLEIDLDLAKNQATRNTSGQILDYISKHLPYIGGSADLSVSTKAK
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCC
GSDGIFSCQNYQGRNIMFGVREFAMASIANGIALHSVLRPFVSTFFVFADYLKPALRLSA
CCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMKLPVSYIFSHDSLMVGEDGPTHEPVEQLAMLRSLPNLAVYRPGDENELKAAYELILAK
HHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCC
KNQPSALILTRQNIKSFNSCKKGFKKGAYLVRKASSKALIATGSELGLANEIANKLDVNL
CCCCCEEEEEHHHHHHHHHHHHHHHHCHHEEEECCCCEEEEECCCCCHHHHHHHHHCEEE
ISLSNWQNGPIDPNYAISLELATTFGKAHAKYNFGHDDFGFSAPADKILEKIGFNFDNLV
EEECCCCCCCCCCCEEEEEEEEEHHCCHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHH
TKIKKIIV
HHHHHHCC
>Mature Secondary Structure
MLKNNIENLAINSLKMHGVAAVNKANSGHPGVILSAAKMVYALYRDHMNFDVENLNINRD
CCCCCCCHHHHHHEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCC
RFILSAGHASGLLYSLLYNLGILEQKDLENFRQMGSKTPGHPEYGHTLGVEATTGPLGQG
EEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCH
IAMGVGLAMAQAHLHSKFPEINHYTYILCGDGDLQEGISYESLSLAGHLKLENLIILYDS
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCCCCCEEEEEEEEEEEEEEEECC
NDIQLDSALDVVFSENVRQRIESQGLFYQLVEKNDVNLISEAIAKAKKSKKPSFIEIKTI
CCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEE
IGEGTSKEKTSDVHGAPLGNDILTLKKNLNEYDDFYLADELRQHKNTLGKRSQKAFAEFK
ECCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISKELKEFLEKGQNLDLEIDLDLAKNQATRNTSGQILDYISKHLPYIGGSADLSVSTKAK
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCC
GSDGIFSCQNYQGRNIMFGVREFAMASIANGIALHSVLRPFVSTFFVFADYLKPALRLSA
CCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMKLPVSYIFSHDSLMVGEDGPTHEPVEQLAMLRSLPNLAVYRPGDENELKAAYELILAK
HHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCC
KNQPSALILTRQNIKSFNSCKKGFKKGAYLVRKASSKALIATGSELGLANEIANKLDVNL
CCCCCEEEEEHHHHHHHHHHHHHHHHCHHEEEECCCCEEEEECCCCCHHHHHHHHHCEEE
ISLSNWQNGPIDPNYAISLELATTFGKAHAKYNFGHDDFGFSAPADKILEKIGFNFDNLV
EEECCCCCCCCCCCEEEEEEEEEHHCCHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHH
TKIKKIIV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 9068642 [H]