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The map label for this gene is pyk

Identifier: 54020111

GI number: 54020111

Start: 280782

End: 282218

Strand: Direct

Name: pyk

Synonym: mhp255

Alternate gene names: 54020111

Gene position: 280782-282218 (Clockwise)

Preceding gene: 54020110

Following gene: 54020112

Centisome position: 31.45

GC content: 33.68

Gene sequence:

>1437_bases
ATGAACGATATGAAAAATTATATTTCAAAAAGAACAAAAATAATCGCAACAATTGGCCCTTCAACGCAGGATTATGAGGT
CCTAAAAAAATTAGTATTATCTGGGGTTAGTGTAATTCGGGCAAATTTTTCCCATGGAAATTATCAAGAGCAAAAACAAA
AATTTGACAGCGCCCGGAAACTTTCTAATGAACTTAAAATTCCAATTTCAATTATGTTAGATACAAAAGGACCCGAAATT
CGCATTGGCAAAATTTTAGAAGGCTCCCAAAAAATCCTTGCAAACCAACGTTTAACTGTCCTAACAGATTCAGATTCTTA
TCAAAATTTTCAAGGAACAGCCCAAATAATTACTGTTTCTCATCAAATTGATAAGGATCTAAAAATCGGTGATAAAATTT
TATTTGATGATGGAAAATTACAATCAGTTGTAATCGAAATTGAGCCCGGAAAAGTTGTAGTTAGAACTAAAAATTCACAT
GTTTTAAAGTCAAATAAGCGACTAAATTTACCCGGTGTAGCATTTTCCTTACCCTTTTTATCCCAAAAAGATATTGATGA
TATTCTTTTCGGGATTAATGAAAATGTTAATTATATAGCTGCATCCTTTGTTAATTCAGGTGAAAATGTCCGTGAATTAC
GCACACTTTTAGATCAAAACGGCGGCGGACATATCCAGATTATCTCCAAAATTGAGTCAAGACTTGGTCTTGAAAATATT
GATGAAATTATTGAATTATCGGATGGAATTATGATTGCCCGTGGCGATCTAGGTCTTGAAGTGCCTTATGAAGAAGTTCC
ATTTCAACAAAAAAGAATTATTCGCAAATGTCGTTTTGCAGGTAAAACTGTTGTAGTTGCAACGCAAATGCTTGATTCAA
TGGAGAAATCATCCCAACCAACAAGGGCAGAAGTAACTGATGTCTATTGAGCAACCGAATTAGGAGCGGATGCGACAATG
CTATCTGGTGAATCTGCCCAGGGTCAGTTCCCAATCGAGTCAGTTCAGGTAATGGCAGTTATTAATAAAAGAGCAGAAAA
AGAATTTTATAACAAACTCTTTTATGCAAAGCAACTGGCAGTAATTACGGAAAATTCAAGGGGACTAAGAGCAAAAATAG
CCCATAAATTAGCGCATTTAGCCTATGAAAAAGAAATAAAATACACAGTAGTGCTATCTCGAACTGGCCAACTTTTAAGG
GAAATTGCAAAATTTAGACCAAATACAGCCGTAATTGGCGTGATTAATAATGAAAAATTAATTAATGGTTTTGGAATTAC
ATCCTCCGTTTTTGTCTCAATTAACTCAATTTCTGAATTTACAGCAATTAAAAATGATTTTAGTCTAGCAAGAAATGTAT
TAGAACCCTTTGGGGTTGAAAAAGGTGATACATTTTTAGTGGTAGAAAACGAAAAAATGGTACAATTTGTTTATTAA

Upstream 100 bases:

>100_bases
ATTAGTAAATTTCTTAATTTTAGTTATAATTATTATTAATTAATTTGCACTTTTTAAACCATTTCAAAATACAAAATACG
AGAAAGTTGCCATCAAAAAA

Downstream 100 bases:

>100_bases
TTTTTTATTTTTTTAATATTTTTTTTGTTTTTCCTGTATAATATAATATGCAAATTTTTATTTGCAGAAAGTAGATAATA
TCTCACCTGAAGATGTTAGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNDMKNYISKRTKIIATIGPSTQDYEVLKKLVLSGVSVIRANFSHGNYQEQKQKFDSARKLSNELKIPISIMLDTKGPEI
RIGKILEGSQKILANQRLTVLTDSDSYQNFQGTAQIITVSHQIDKDLKIGDKILFDDGKLQSVVIEIEPGKVVVRTKNSH
VLKSNKRLNLPGVAFSLPFLSQKDIDDILFGINENVNYIAASFVNSGENVRELRTLLDQNGGGHIQIISKIESRLGLENI
DEIIELSDGIMIARGDLGLEVPYEEVPFQQKRIIRKCRFAGKTVVVATQMLDSMEKSSQPTRAEVTDVYWATELGADATM
LSGESAQGQFPIESVQVMAVINKRAEKEFYNKLFYAKQLAVITENSRGLRAKIAHKLAHLAYEKEIKYTVVLSRTGQLLR
EIAKFRPNTAVIGVINNEKLINGFGITSSVFVSINSISEFTAIKNDFSLARNVLEPFGVEKGDTFLVVENEKMVQFVY

Sequences:

>Translated_478_residues
MNDMKNYISKRTKIIATIGPSTQDYEVLKKLVLSGVSVIRANFSHGNYQEQKQKFDSARKLSNELKIPISIMLDTKGPEI
RIGKILEGSQKILANQRLTVLTDSDSYQNFQGTAQIITVSHQIDKDLKIGDKILFDDGKLQSVVIEIEPGKVVVRTKNSH
VLKSNKRLNLPGVAFSLPFLSQKDIDDILFGINENVNYIAASFVNSGENVRELRTLLDQNGGGHIQIISKIESRLGLENI
DEIIELSDGIMIARGDLGLEVPYEEVPFQQKRIIRKCRFAGKTVVVATQMLDSMEKSSQPTRAEVTDVY*ATELGADATM
LSGESAQGQFPIESVQVMAVINKRAEKEFYNKLFYAKQLAVITENSRGLRAKIAHKLAHLAYEKEIKYTVVLSRTGQLLR
EIAKFRPNTAVIGVINNEKLINGFGITSSVFVSINSISEFTAIKNDFSLARNVLEPFGVEKGDTFLVVENEKMVQFVY
>Mature_478_residues
MNDMKNYISKRTKIIATIGPSTQDYEVLKKLVLSGVSVIRANFSHGNYQEQKQKFDSARKLSNELKIPISIMLDTKGPEI
RIGKILEGSQKILANQRLTVLTDSDSYQNFQGTAQIITVSHQIDKDLKIGDKILFDDGKLQSVVIEIEPGKVVVRTKNSH
VLKSNKRLNLPGVAFSLPFLSQKDIDDILFGINENVNYIAASFVNSGENVRELRTLLDQNGGGHIQIISKIESRLGLENI
DEIIELSDGIMIARGDLGLEVPYEEVPFQQKRIIRKCRFAGKTVVVATQMLDSMEKSSQPTRAEVTDVY*ATELGADATM
LSGESAQGQFPIESVQVMAVINKRAEKEFYNKLFYAKQLAVITENSRGLRAKIAHKLAHLAYEKEIKYTVVLSRTGQLLR
EIAKFRPNTAVIGVINNEKLINGFGITSSVFVSINSISEFTAIKNDFSLARNVLEPFGVEKGDTFLVVENEKMVQFVY

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=441, Percent_Identity=37.6417233560091, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI32967597, Length=441, Percent_Identity=37.6417233560091, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI33286422, Length=480, Percent_Identity=35.8333333333333, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI33286420, Length=480, Percent_Identity=35.8333333333333, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI33286418, Length=481, Percent_Identity=35.966735966736, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI310128732, Length=295, Percent_Identity=37.9661016949153, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI310128730, Length=295, Percent_Identity=37.9661016949153, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=34.5991561181435, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=34.5991561181435, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.4883720930233, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1787965, Length=468, Percent_Identity=39.1025641025641, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI1788160, Length=342, Percent_Identity=36.2573099415205, Blast_Score=198, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17544584, Length=424, Percent_Identity=35.6132075471698, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI71984413, Length=357, Percent_Identity=40.6162464985994, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17506829, Length=357, Percent_Identity=40.6162464985994, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI71984406, Length=357, Percent_Identity=40.6162464985994, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17506831, Length=357, Percent_Identity=40.6162464985994, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=472, Percent_Identity=36.4406779661017, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324923, Length=467, Percent_Identity=36.4025695931477, Blast_Score=260, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24648964, Length=415, Percent_Identity=38.0722891566265, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI28571814, Length=415, Percent_Identity=38.0722891566265, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24648966, Length=403, Percent_Identity=35.9801488833747, Blast_Score=238, Evalue=7e-63,
Organism=Drosophila melanogaster, GI24581235, Length=418, Percent_Identity=32.0574162679426, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24646914, Length=246, Percent_Identity=36.1788617886179, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53220; Mature: 53220

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDMKNYISKRTKIIATIGPSTQDYEVLKKLVLSGVSVIRANFSHGNYQEQKQKFDSARK
CCHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHH
LSNELKIPISIMLDTKGPEIRIGKILEGSQKILANQRLTVLTDSDSYQNFQGTAQIITVS
HHCCCCCEEEEEEECCCCCEEEEHHHCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEEEE
HQIDKDLKIGDKILFDDGKLQSVVIEIEPGKVVVRTKNSHVLKSNKRLNLPGVAFSLPFL
HHCCCCCCCCCEEEECCCCEEEEEEEECCCEEEEEECCCEEEECCCCCCCCCCEEECCCC
SQKDIDDILFGINENVNYIAASFVNSGENVRELRTLLDQNGGGHIQIISKIESRLGLENI
CCCCHHHHHHCCCCCCCEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHCCHHHH
DEIIELSDGIMIARGDLGLEVPYEEVPFQQKRIIRKCRFAGKTVVVATQMLDSMEKSSQP
HHHHHHCCCEEEEECCCCCCCCHHHCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCC
TRAEVTDVYATELGADATMLSGESAQGQFPIESVQVMAVINKRAEKEFYNKLFYAKQLAV
CCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
ITENSRGLRAKIAHKLAHLAYEKEIKYTVVLSRTGQLLREIAKFRPNTAVIGVINNEKLI
EECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCCEEEEEECCCEEE
NGFGITSSVFVSINSISEFTAIKNDFSLARNVLEPFGVEKGDTFLVVENEKMVQFVY
ECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEC
>Mature Secondary Structure
MNDMKNYISKRTKIIATIGPSTQDYEVLKKLVLSGVSVIRANFSHGNYQEQKQKFDSARK
CCHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHH
LSNELKIPISIMLDTKGPEIRIGKILEGSQKILANQRLTVLTDSDSYQNFQGTAQIITVS
HHCCCCCEEEEEEECCCCCEEEEHHHCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEEEE
HQIDKDLKIGDKILFDDGKLQSVVIEIEPGKVVVRTKNSHVLKSNKRLNLPGVAFSLPFL
HHCCCCCCCCCEEEECCCCEEEEEEEECCCEEEEEECCCEEEECCCCCCCCCCEEECCCC
SQKDIDDILFGINENVNYIAASFVNSGENVRELRTLLDQNGGGHIQIISKIESRLGLENI
CCCCHHHHHHCCCCCCCEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHCCHHHH
DEIIELSDGIMIARGDLGLEVPYEEVPFQQKRIIRKCRFAGKTVVVATQMLDSMEKSSQP
HHHHHHCCCEEEEECCCCCCCCHHHCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCC
TRAEVTDVYATELGADATMLSGESAQGQFPIESVQVMAVINKRAEKEFYNKLFYAKQLAV
CCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
ITENSRGLRAKIAHKLAHLAYEKEIKYTVVLSRTGQLLREIAKFRPNTAVIGVINNEKLI
EECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCCEEEEEECCCEEE
NGFGITSSVFVSINSISEFTAIKNDFSLARNVLEPFGVEKGDTFLVVENEKMVQFVY
ECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]